Malaysian
Journal of Analytical Sciences Vol 20 No 2 (2016): 388 - 392
CHEMICAL CONSTITUENTS FROM THE LIANAS OF Gnetum cuspidatum Blume
(Sebatian Kimia
dari Lianas Gnetum cuspidatum Blume)
Nik Fatini Nik Azmin1,
Norizan Ahmat1,2*, Nik Khairunissa Nik Abdullah Zawawi1,2
1Faculty of Applied Sciences,
Universiti
Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
2Atta-ur-Rahman Institute for Natural Product
Discovery,
Universiti
Teknologi MARA, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia
*Corresponding author: noriz118@salam.uitm.edu.my
Received: 24
February 2015; Accepted: 27 October 2015
Abstract
Gnetum is
a genus of gymnosperms, the sole
genus in the family Gnetaceae with approximately 40 species.
Various species has been used for the treatment of rheumatitis, arthritis, bronchitis and asthma in folk
medicines. Gnetum
cuspidatum Blume is
known throughout tropical Southeast Asia from Thailand, Vietnam, Cambodia,
Malaysia, Sumatra, Java, and Borneo to the Maluku, Sulawesi and New Guinea. In
this research work, a methanol extract of the lianas of Gnetum cuspidatum was subjected to vacuum liquid chromatography for
fractionation. Later, several selective fractions had undergone the repetitive
radial chromatography technique for further purification. Four known
constituents categorized as stilbene type of compound have been successfully
isolated and identified which include resveratrol (1), gnetucleistol C (2),
gnetucleistol D (3) and gnemonol M (4). The structures and configuration of the
reported compounds were elucidated on the basis of 2D-NMR correlations and
comparison with the literature.
Keywords:
Gnetaceae, Gnetum, Gnetum cuspidatum, stilbene
Abstrak
Gnetum merupakan genus gymnosperms, genus tunggal dalam
keluarga Gnetaceae dengan kira-kira 40 spesies. Pelbagai spesies telah
digunakan untuk rawatan rheumatitis, arthritis, bronkitis dan asma dalam
perubatan penduduk tempatan. Gnetum
cuspidatum Blume dikenali di seluruh tropika Asia Tenggara dari Thailand,
Vietnam, Kemboja, Malaysia, Sumatera, Jawa, Borneo dan ke Maluku, Sulawesi dan
New Guinea. Satu ekstrak metanol daripada lianas Gnetum cuspidatum telah menggunakan vakum kromatografi cecair untuk
pemisahan. Kemudian, beberapa pemisahan yang terpilih telah menjalani teknik
kromatografi jejarian secara berulang kali untuk penulenan lanjut. Empat
sebatian yang dikategorikan sebagai jenis stilbene telah berjaya diasingkan dan
dikenalpasti iaitu resveratrol (1), gnetucleistol C (2), gnetucleistol D (3)
dan gnemonol M (4). Struktur dan konfigurasi sebatian yang telah dilapor ini
berdasarkan korelasi 2D-NMR dan perbandingan dengan kajian literatur.
Kata kunci: Gnetaceae,
Gnetum, Gnetum cuspidatum, stilben
References
1.
Medicinal Herb Index in Indonesia. (1995). PT. EISAI Indonesia,
Second Edition.
2.
Shimizu, K., Kondo, R. and Sakai. K. (2000). Inhibition of tyrosinase by
flavonoids, stilbenes and related 4-substituted resorcin. Planta Medica, 66: 11–15.
3.
Lojanapiwatna, V., Mahaeja, J. and P. Wiriyachitra. (1982).
Isolation of (E)- 3 ,4,
5-trihydroxy-3’-methoxystilbene from Gnetum
cuspidatum Bl. Journal of the Science
Society of Thailand, 8(1): 59 – 63.
4.
Shimokawa, Y., Hirasawa, Y., Kaneda, T., Hamid, A., Hadi, A. and H.
Morita. (2012). Cuspidans A and B, Two
New Stilbenoids from the Bark of Gnetum cuspidatum. Chemical and Pharmaceutical Bulletin, 60(6):
790 – 792.
5.
Huang, K. S. and Lin, M. (1999).
Oligostilbenes from the Roots of Vitis amurensis. Journal of
Asian Natural Products Research, 2: 21 – 28.
6.
Yao, C. S., Lin, M., Liu, X. and Wang, Y. H. (2005). Stilbene
derivatives from Gnetum cleistostachyum.
Journal of Asian Natural Products Research, 7: 131 – 137.
7.
Yao, C. S., Lin, M., Liu, X. and Wang, Y. H. (2003). Stilbene from Gnetum cleistostachyum. Acta Chimica
Sinica,
61 (8): 1331 – 1334.
8.
Ilya, I., Ali, Z., Tanaka, T., Iinuma, M., Furusawa, M., Nakaya,
K., Murata, J., Darnaedi, D., Matsuura, N. and Ubukata, M. (2003). Stilbene
derivatives from Gnetum gnemon Linn. Phytochemistry, 62: 601 – 606.