Malaysian Journal of Analytical Sciences Vol 20 No 2 (2016): 238 - 246

 

 

 

TWO ISOLATED CRYSTALLINE SOLIDS FROM THE STEM OF Entada spiralis Ridl. (AKAR SINTOK)

 

(Pemencilan Dua Jenis Pepejal Kristal dari Batang Pokok Entada spiralis (Akar Sintok))

 

Aiza Harun¹*, Siti Zaiton Mat So’ad², Norazian Mohd Hassan²

 

¹Faculty of Applied Sciences,

Universiti Teknologi MARA Pahang, 26400 Bandar Tun Razak Jengka, Pahang, Malaysia

²Kulliyah of Pharmacy,

International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan Pahang, Malaysia

 

*Corresponding author: aizaharun@pahang.uitm.edu.my

 

 

Received: 30 November 2015; Accepted: 1 February 2016

 

 

Abstract

Entada spiralis Ridl. (Leguminosae) is a woody climber which locally known as ‘akar sintok’. The isolation and structure determination of two crystalline solids from active methanol fraction of E. spiralis stem bark had been examined.  The structure identification of both solids was based on spectroscopic data (¹H and ¹³C NMR, HMQC, HMBC, DEPT135 and MS) along with comparison with literature data. The results revealed that these two crystalline solid sugars isolated from the most active fraction were known as penta-2-acetoxy-β-D-digitoxopyranosyl-(1→2)-fructofuranosyl-(6→4)-β-D-glucopyranosyl-(1→4)-glucopyranosyl-(1→2)-β-D fructofuranosyl-(6→1)-β-D-glucopyranosyl-(4→1)-acetylglucosamine (1) and  β,D-glucopyra-nosyl(1→2)-β,D-glucopyranosyl)-(1→3)-β,D-xylopyranosyl(1→4)-α,L-rhamnopyranosyl)-(1→3)-β,D- glucopyranosyl(1→3)-β,D-glucopyranoside (2) Thus, this finding can be utilized as a scientific baseline information for further skin disease studies, since both constituents were isolated from moderate antidermatophytic active fraction.

 

Keywords: Entada spiralis, crystalline saponin, glycosides, isolation

 

Abstrak

Pemencilan dan penentuan struktur dua komponen bersifat kristal dari batang pokok E. spiralis yang juga dikenali sebagai akar sintok telah dijalankan. Eksperimen penentuan struktur berdasarkan data – data spektroskopi menggunakan ¹H, ¹³C NMR, HMQC, HMBC, DEPT135 dan spektrometer jisim dan juga perbandingan dengan kajian terdahulu. Hasil analisa spektroskopi yang terperinci menunjukkan kedua – dua kristal tersebut dikenali sebagai penta-2-asetoksi-β-D-digitoxopiranosil-(1→2)-fruktofuranosil-(6→4)-β-D-glukopiranosil-(1→4)-glukopiranosil-(1→2)-β-D fruktofuranosil-(6→1)-β-D-glukopiranosil-(4→1)-acetilglukosamina (1) and  β,D-glukopiranosil(1→2)-β,D-glukpiranosil)-(1→3)-β,D-xilopiranosil(1→4)-α,L-rhamnopiranosil)-(1→3)-β,D-glukopiranosil(1→3)-β,D-glukopiranosid (2). Penemuan ini boleh dijadikan maklumat asas untuk kajian penyakit kulit kerana kedua-dua komponen ini dipencilkan dari fraksi metanol yang bersifat antiderma.

 

Kata kunci: Entada spiralis, kristal saponin, glikosid, pemencilan

 

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