Malaysian Journal of Analytical Sciences Vol 20 No 3 (2016): 573 - 577

DOI: http://dx.doi.org/10.17576/mjas-2016-2003-16

 

 

 

ISOLATION OF ACETYL ALEURITOLIC ACID FROM Pimeleodendron griffithianum (Euphorbiaceae)

 

(Pemisahan Asid Asetil Aleuritolik Daripada Pimeleodendron griffithianum (Euphorbiaceae))

 

Rosmawati Abdul Aziz^{1, 2}* and Norizan Ahmat^{1, 2}

 

¹Faculty of Applied Sciences,

Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia

²Atta-ur-Rahman Institute for Natural Product Discovery,

Universiti Teknologi MARA, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.

 

*Corresponding author: rosmawatiaa@perlis.uitm.edu.my

 

 

Received: 24 February 2015; Accepted: 27 October 2015

 

 

Abstract

Pimeleodendron griffithianum, locally known as Perah ikan is available in Thailand, Peninsular Malaysia and Sumatra. The plant can be found in wet forest, secondary forest, pole forest, semi-swamps, and along logging roads or on hill slopes. In Malaysia, the stem of P. griffithianum being used as commercial timber. So far, no chemical constituents have been reported from P. griffithianum. In this study, a phytochemical study was conducted on the stem bark of P. griffithianum. The stem bark was obtained from Sungai Siput, Perak. The cleaned, chopped and dried stem-bark was extracted using acetone for several times. The separation of the components was carried out using vacuum liquid chromatography (VLC) followed by recrystallization method. The structure of the pure compound was elucidated by spectroscopic methods including nuclear magnetic resonance (NMR), ultraviolate – visible (UV-Vis) and comparison with literature. One compound (1) was isolated and identified as acetyl aleuritolic acid. This compound was reported for the first time from this plant.

 

Keywords: Pimeleodendron griffithianum, Euphorbiaceae, acetyl aleuritolic acid

 

Abstrak

Pimeleodendron griffithianum, yang dikenali sebagai pokok Perah ikan oleh penduduk tempatan didapati di Thailand, Semenanjung Malaysia dan Sumatra. Pokok ini dapat ditemui di dalam hutan lembap, hutan sekunder, semi-paya dan di sepanjang jalan pembalakan atau di cerun bukit. Di Malaysia, batang pokok ini lazimnya digunakan sebagai kayu komersial. Setakat ini tidak ada laporan direkodkan mengenai bahan kimia yang ditemui daripada pokok ini. Dalam penyelidikan ini, satu kajian fitokimia telah dijalankan ke atas kulit batang P. griffhianum. Kulit batang pokok P.griffithianum diperolehi dari Sungai Siput, Perak. Kulit pokok yang telah dibersihkan, dipotong-potong kecil dan dikeringkan, diekstrak menggunakan aseton untuk beberapa kali.  Pengasingan komponen dilakukan menggunakan kaedah kromatografi cecair vakum (VLC) diikuti dengan proses penghabluran semula. Struktur kimia sebatian tulen dijelaskan dengan kaedah spektroskopi termasuk spektroskopi resonans magnet nukleus (NMR), ultralembayung (UV) dan perbandingan dengan data literatur. Satu sebatian (1) yang dipisah dikenal pasti sebagai asid asetil aleuritolik. Sebatian ini dilaporkan buat kali pertama bagi tumbuhan ini.

 

Kata kunci: Pimeleodendron griffithianum, Euphorbiaceae, asid asetil aleuritolik

 

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