Malaysian Journal of Analytical Sciences Vol 20 No 5 (2016): 1203 - 1216

DOI: http://dx.doi.org/10.17576/mjas-2016-2005-27

 

 

 

ANTIULCER ACTIVITY OF Musa paradisiaca (BANANA) TEPAL AND SKIN EXTRACTS IN ULCER INDUCED ALBINO MICE

 

(Aktiviti Antiulser dari Ekstrak Jantung dan Kulit Musa paradisiaca (Pisang) pada Tikus Albino Teraruh Ulser)

 

U.S Mahadeva Rao¹, Bashir Ado Ahmad¹*^, Khamsah Suryati Mohd^2,3, Thant Zin¹

 

¹Faculty of Medicine,

Universiti Sultan Zainal Abidin, Medical Campus, 21400 Kuala Terengganu, Terengganu, Malaysia

²Faculty of Bioresources and Food Industry

³Agriculture Production and Food Innovation Research Institute

Universiti Sultan Zainal Abidin, Tembila Campus, 22200 Besut, Terengganu, Malaysia

 

*Corresponding author: dambash011@yahoo.com

 

 

Received: 14 April 2015; Accepted: 3 August 2016

 

 

Abstract

The current use of ulcer drugs is limited due to its side effects and potentiality of relapse. This study aimed to ascertain the antiulcer potentials of the tepal and skin extracts of banana Musa paradisiaca. The parts were processed and extracted using methanol by maceration. Phytochemicals content of both parts were screened. Twenty-five albino mice were used in in vivo analysis. The mice were treated with 100 mg/kg of tepal and skin extract as well as cimetidine for seven days followed by administration of indomethacin. The animals were sacrificed, and the removed stomachs were prepared for the evaluation of ulcer index and gastric wall mucus. pH and volume were measured from the gastric juice. The results revealed that banana’s tepal and skin extracts contain phytochemicals like phenols, flavonoids and etc. The tepal and skin extracts prevented the IND+PYL induced ulcer by 68.80 ± 20.53% and 43.22 ± 14.82% respectively. Significant rise (p <0.05) in gastric juice pH (3.79 ± 0.24) was noticed in the banana’s tepal treated group. However, the decrease in gastric juice volume and increased gastric wall mucus by both tepal and skin were not statistically significant (p >0.05). Findings from this study shows that banana’s tepal and skin were able to prevent IND+PYL induced ulcer by strengthening the gastric mucosa and decreasing the gastric juice acidity.

 

Keywords:  banana’s tepal, banana’s skin, indomethacin, Musa paradisiaca, preventive index.

 

Abstrak

Penggunaan ubat ulser pada masa kini adalah terhad disebabkan kesan sampingan dan potensi kambuh. Kajian ini bertujuan menentukan potensi antiulser dari ekstrak jantung dan kulit pisang Musa paradisiaca. Bahagian – bahagian ini telah diproses dan diekstrak menggunakan metanol melalui kaedah rendaman. Kandungan fitokimia kedua – dua bahagian telah disaring. Dua puluh lima tikus albino telah digunakan dalam analisis in vivo. Tikus – tikus ini telah diberi rawatan dengan 100mg/kg ekstrak jantung dan kulit pisang serta simetidin selama tujuh hari di ikuti dengan rawatan dengan indomethacin. Haiwan dikorbankan selepas 8 hari rawatan, dan perut dikeluarkan bagi penilaian indeks ulser dan mukus dinding perut. pH dan jumlah jus gastrik juga diukur. Hasil kajian mendapati bahawa ekstrak jantung dan kulit pisang mengandungi beberapa fitokimia seperti fenol, flavonoid, dan lain – lain. Ekstrak jantung dan kulit ini menghalang ulser yang diaruh dengan IND+PYL masing – masing sebanyak 68.80 ± 20.53% dan 43.22 ± 14.82%. Peningkatan yang signifikan (p <0.05) pada pH jus gastrik (3.79 ± 0.24) telah diperhatikan dalam kumpulan yang dirawat dirawat dengan jantung pisang. Walau bagaimanapun, penurunan dalam jumlah jus gastrik dan meningkatkan mukus dinding perut oleh kedua – dua ekstrak adalah tidak signifikan secara statistik (p >0.05). Dapatan dari kajian ini menunjukkan bahawa jantung dan kulit pisang dapat mencegah ulser yang diaruh dengan IND+PYL melalui penambahan mukosa perut dan pengurangan keasidan jus gastrik.

 

Kata kunci:  jantung pisang, kulit pisang, indomethacin, Musa paradisiaca, indeks pencegahan

 

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