Malaysian Journal of Analytical Sciences Vol 21 No 2 (2017): 291 - 297

DOI: https://doi.org/10.17576/mjas-2017-2102-03

 

 

 

THE CHEMICAL CONSTITUENTS FROM YOUNG TUBERS of  Hydnophytum formicarum

 

(Juzuk Kimia Daripada Tuber Muda Hydnophytum formicarum)

 

Nur Shafiqa Abdullah, Wan Yaacob Wan Ahmad*, Noor Aziiraa Sabri

 

School of Chemical Sciences and Food Technology,

Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

 

*Corresponding author: wanyaa@ukm.edu.my

 

 

Received: 6 December 2016; Accepted: 16 January 2017

 

 

Abstract

The fine powders of young tubers of Hydnophytum formicarum was extracted by Soxhlet methanol extraction for 18 hours in three days continuously. The filtrate then was evaporated by rotary evaporator until it became concentrated solution. The fractionation and purification of the extract by vacuum liquid chromatography and radial chromatography has led to the discovery of four compounds namely sinapinic acid, β-sitosterol acetate, β-sitosterol and stigmasterol. Structures of the compounds were established by interpreting mass spectral data, ¹H and ¹³C-APT NMR, Infrared and by comparison with literature data.

 

Keywords:  Hydnophytum formicarum, sinapinic acid, β-sitosterol acetate, β-sitosterol, stigmasterol

 

Abstrak

Serbuk halus tuber muda Hydnophytum formicarum telah diekstrak melalui pengekstrakan Soxhlet selama 18 jam dalam tiga hari secara berturutan. Hasil penurasan kemudian telah disejat menggunakan penyejat berputar sehingga menjadi larutan yang likat. Pemfraksian dan penulenan ekstrak menggunakan kromatografi cecair vakum dan kromatografi radial membawa kepada penemuan empat sebatian yang bernama asid sinapinik, β-sitosterol asetat, β-sitosterol dan stigmasterol. Struktur sebatian ini telah ditentukan dengan mentafsirkan data spektrum jisim, RMN ¹H dan ¹³C-APT, Inframerah serta secara membandingkannya dengan data kepustakaan.

 

Kata kunci:  Hydnophytum formicarum, asid sinapinik, β-sitosterol asetat, β-sitosterol, stigmasterol

 

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