UKM Medical Molecular Biology Institute

 Leading Institute in Molecular Medicine

miRNA PCR Array Workshop

 

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    OBJECTIVES

    • To introduce to participants on exosomes and exosomal RNA
    • To introduce to the participants the basics of doing PCR Array
    • To expose the applications of PCR Array in an experimental design
    • To support and asssist research related to molecular biology and molecular medicine

  • Time Day 1 Day 2
    8:00 amRegistration and breakfastBreakfast
    9:00 amLecture 1:
    Introduction to PCR Array and Examples of Applications
    (Seminar room, Level 5)
    		Lecture 3: UMBI's Experience in miRNA PCR Array (Seminar Room, Level 5)
    10:00 amLecture 2:
    Tips and tricks for exosomal studies (Seminar room, Level 5)    		Hands-on 3:
    PCR Array
    (MBU, Level 4)
    11.00 amHands-on 1: Exosomal RNA Isolation
    1:00 pmLunch
    (Level 1)
    		Lunch
    (Level 1)
    2:30 pmHands-on 2:
    cDNA synthesis
    (MBU, Level 4)
    		Lecture 4:
    Knockdown analysis of miRNA by Qiagen (Seminar Room, Level 5)
    3:30 pmHands-on 4:
    Data analysis (Bioinformatics Lab, Level 3)
    4:00 pmDay 1 Review and TeaDay 2 Review and Tea

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    Dr Chiam Chun Wei, QIAGEN Application Specialist, Malaysia

    Dr Chun Wei obtained his PhD at University of Malaya for his study on “Neurovirulence variation among the two genotypes of chikungunya virus in Malaysia”. He has 5 years of experience in laboratory, 6 publications, 4 poster presentations and 1 book chapter. He was awarded with 2 travel grants to attend 9th Asian Pacific Congress of Medical Virology at Adelaide, Australia in 2012 and 16th Biological Sciences Graduate Congress at National University of Singapore in 2011. He joined QIAGEN Biotechnology Malaysia Sdn. Bhd. since 2016 as the Application Specialist for life science and biological content research portfolios.

     

    Dr. Nadiah Abu, Research Fellow/Senior Lecturer, UMBI

    Dr. Nadiah is a research fellow from the functional genomics group in UMBI. She obtained her PhD from Universiti Putra Malaysia in 2015 focusing on drug discovery. Her current research interests is regarding extracellular vesicles and its applications as biomarkers and immune regulators in cancers. She has received several grants for researching on exosomes among which is a study to profile non coding RNAs in exosomes derived from colorectal cancer cells. She has also published and presented her work in exosomes and extracellular vesicles.

    Dr. Siti Aishah Sulaiman, Research Fellow/Senior Lecturer, UMBI

    Dr Siti Aishah Sulaiman earned her PhD in Medicine from The University of Adelaide, Australia in 2015, with a thesis title of “Early Life Determinants of β-Cell Function in The Sheep”. In her PhD project, one of the aims was to determine the pancreatic islet microRNA expression profile and how they are correlated with the expression and methylation of the key genes in β-cells function. Following that, she joined the UKM Medical Molecular Biology Institute (UMBI) as a research fellow. Here in UMBI, she has been involved in various projects related to Diabetes and its complications, with a focus on the role of non-coding RNAs. She once used the Qiagen qPCR array to determine the oxidative stress role in Bipolar Disorder patients, as part of the collaborative research with the Psychiatry Department. Dr Siti Aishah has numerous experiences in performing the molecular experiment and the data analysis.

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    Institut Perubatan Molekul UKM (UMBI)
    Jalan Yaacob Latiff, Bandar Tun Razak
    56000 Cheras, Kuala Lumpur, Malaysia
    Fax : +603 – 9171 7185

    For any inquiry, please directly contact Nadiah (nadiah.abu@ppukm.ukm.edu.my); Nurmi (ctnurminasir@ppukm.ukm.edu.my)

  • The UMBI-QIAGEN REAL TIME PCR WORKSHOP – GENE EXPRESSION ANALYSIS will be held at UKM Medical Molecular Biology Institute (UMBI), Kuala Lumpur that is located just a 15-minute drive away from the heart of Kuala Lumpur city center.

    For more information about the venue, please visit the UMBI website at https://www.ukm.my/umbi/.

    Please take note that the accommodation is NOT provided. Kindly refer to the Accommodation tab for further information.

     

    WAZE: https://www.waze.com/directions/malaysia/kuala-lumpur/umbi-ukm/66650143.666632502.6392182.html

    GOOGLE MAP: https://goo.gl/maps/soeAgH9K4292

  • Hotels nearby UMBI:

    1. Hotel Sri Permaisuri Cheras (3-star hotel)
    2. Hotel EST Kuala Lumpur
    3. International Youth Centre (IYC) Residence

    Low Budget hotel are also available nearby the UMBI. Please refer to any hotel booking website for further information.

    **Important Note : KINDLY BE NOTED THAT THE ORGANIZER WILL NOT BE LIABLE FOR ANY CLAIM OR RESPONSIBILITY IN REGARDS OF THE LISTED ACCOMMODATION ABOVE. FOR ACCOMMODATION BOOKING AND RESERVATION, PLEASE CLICK THE APPROPRIATE LINKS AND FOLLOW THE GUIDELINES IN THE RESPECTIVE WEBSITE.

  • Nadiah (nadiah.abu@ppukm.ukm.edu.my), Nurmi (ctnurminasir@ppukm.ukm.edu.my)
    Institut Perubatan Molekul UKM (UMBI)
    Jalan Yaacob Latiff, Bandar Tun Razak
    56000 Cheras, Kuala Lumpur, Malaysia
    Fax : +603 – 9171 7185

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