ID
RTPCR01
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Intro
Nucleic acid amplification and detection techniques are among the most valuable tools in biological research. RT-PCR is used to detect and quantify RNA. Total RNA is first transcribed into complementary DNA (cDNA).
The cDNA is then used as the template for the quantitative PCR or real-time PCR reaction (qPCR). In qPCR, the amount of amplification product is measured in each PCR cycle using fluorescence.
Application
● Gene expression analysis
● Genetically testing,
● Cancer phenotyping
● High resolution melt analysis (HRM)
● SNP genotyping
● RNAi validation,
● microarray validation,
● pathogen detection,
● MikroRNAs (miRNAs) quantificatian and profiling
Technical
Specification
● Ramping rates up to 2.5°C/sec
● Temperature range 0 – 100°C
● Multiplex analysis: up to 4 targets per well
● Sample capacity: 384 wells
● High-throughput
Location
Makmal Proteomik & Biologi Molekul, Bangunan Praklinikal, Fakulti Perubatan UKM
1-30 uL (5-20uL recommended) samples volumes
Contact
ABOUT US
The Faculty of Medicine UKM was established in 1974 and commenced with 44 students for the 1973/74 admissions session.
OFFICE ADDRESS
Secretariat of Research and Innovation
Faculty of Medicine UKM
Level 6, Preclinical Building
Jalan Yaacob Latif, Bandar Tun Razak
56000 Cheras, Wilayah Persekutuan Kuala Lumpur.