ID
RTPCR03
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Intro
Nucleic acid amplification and detection techniques are among the most valuable tools in biological research. RT-PCR is used to detect and quantify RNA. Total RNA is first transcribed into complementary DNA (cDNA).
The cDNA is then used as the template for the quantitative PCR or real-time PCR reaction (qPCR). In qPCR, the amount of amplification product is measured in each PCR cycle using fluorescence.
Application
● Gene expression analysis
● Genetically testing,
● Cancer phenotyping
● High resolution melt analysis (HRM)
● SNP genotyping
● RNAi validation,
● microarray validation,
● pathogen detection,
● MikroRNAs (miRNAs) quantificatian and profiling
Technical
Specification
• Optical system: 5 channels plus HRM; Excitation sources: high-energy light-emitting diodes; Detector: photomultiplier; Acquisition time: 4 seconds
• Sample per run: tubes 0.2 ml, 36 samples/run; Strip Tubes 0.1 ml (4 tubes), 72 samples/run; Rotor-Disc 72, 72 samples/run; Rotor-Disc 100, 100 samples/run
• Thermal performance: Temperature range, 35–99°C (95–210.2°F)
• Software: Rotor Gen-Q software
Location
Makmal General 1, Jabatan Mikrobiologi Dan Imunologi Perubatan, Bangunan Praklinikal, Fakulti Perubatan UKM
1-30 uL (5-20uL recommended) samples volumes *
1- 50 uL (5-200 uL recommended) samples volumes *
* Depends on the rotor usage
Contact
ABOUT US
The Faculty of Medicine UKM was established in 1974 and commenced with 44 students for the 1973/74 admissions session.
OFFICE ADDRESS
Secretariat of Research and Innovation
Faculty of Medicine UKM
Level 6, Preclinical Building
Jalan Yaacob Latif, Bandar Tun Razak
56000 Cheras, Wilayah Persekutuan Kuala Lumpur.