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Sains Malaysiana 49(1)(2020): 49-55
http://dx.doi.org/10.17576/jsm-2020-4901-06
Simple
Extraction of Bisphenol A in Beverages and Water by Membrane-Protected Liquid
Phase Microextraction
(Pengekstrakan
Mudah Bisfenol A dalam Minuman dan Air menggunakan Pengekstrakan Mikro Fasa
Cecair Dilindungi Membran)
THUNG
YING LOH, WAN MOHD AFIQ WAN MOHD KHALIK & SAW HONG LOH*
Faculty
of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala
Terengganu, Terengganu Darul Iman, Malaysia
Received: 24 June
2019/Accepted: 2 October 2019
ABSTRACT
Bisphenol A (BPA) is a xenoestrogen that widely used in manufacture of plastics. Concern is mounting up when
the application of BPA is widespread. A simple sample pre-treatment technique
termed membrane-protected liquid phase microextraction (MP-LPME) combined with
high performance liquid chromatography (HPLC)-fluorescence detection (FD) was
developed for the determination of BPA in beverages and environmental water
samples. The MP-LPME technique utilized a nylon membrane to hold the extractant
(1-octanol) and prevent the extractant from dissolution into sample solution during
agitation. Under optimal extraction conditions, the MP-LPME-HPLC-FD
demonstrated ultra-trace detection of BPA and insignificant matrix effect
extraction with good relative recoveries in the range of 87.1-99.7% from spiked
beverage and environmental water samples. The membrane can be re-applied in the
MP-LPME after the first usage and offered relative recovery of > 94%. The
MP-LPME technique is eco-friendly as it consumes only minute amounts of organic
solvent which is supportive to green analysis.
Keywords: Bisphenol A; HPLC; liquid phase
microextraction; nylon membrane
ABSTRAK
Bisfenol
A (BPA) ialah xenoestrogen yang digunakan secara meluas dalam pembuatan
plastik. Kebimbangan semakin meningkat apabila aplikasi BPA tersebar luas. Satu
teknik pra-rawatan sampel yang mudah dikenali sebagai pengekstrakan
mikro fasa cecair yang dilindungi membran (MP-LPME) berpasangan kromatografi
cecair prestasi tinggi-pengesanan pendarfluor (HPLC-FD) telah dibangunkan untuk
penentuan BPA dalam sampel minuman and air alam sekitar. Teknik MP-LPME
menggunakan membran nilon untuk memegang ekstrak (1-oktanol) dan mencegah
ekstrak daripada pemelarutan ke dalam larutan sampel semasa pengacauan. Di
bawah keadaan pengekstrakan optimum,
MP-LPME-HPLC-FD menunjukkan pengesanan ultra surih dan pengekstrakan kesan
matriks yang tidak signifikan dengan purata pemulihan semula relatif yang baik
dalam linkungan 87.1-99.7% daripada sampel minuman dan air alam sekitar.
Membran ini boleh digunakan semula dalam MP-LPME selepas penggunaan pertama dan
menawarkan pemulihan semula relatif > 94%. Teknik MP-LPME adalah mesra alam
kerana ia hanya menggunakan sedikit pelarut organik dan menyokong analisis
hijau.
Kata kunci: Bisfenol A; HPLC; membran
nilon; pengekstrakan mikro fasa cecair
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*Corresponding author; email: lohsh@umt.edu.my
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