Sains Malaysiana 49(7)(2020): 1597-1613

http://dx.doi.org/10.17576/jsm-2020-4907-12

High-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (HPLC-QTOFMS) Analysis on the Ethanol:Water (80:20) Extract of Lawsonia inermis  Leaves

(Analisis Cecair Kromatografi Berprestasi Tinggi Kuadrupol Spektrometri Jisim Masa Penerbangan (HPLC-QTOFMS) pada Etanol:Air (80:20) Ekstrak daun Lawsonia inermis)

MUHAMMAD REMY OTHMAN^1,2*, ROZANA OTHMAN^3,4, ABDUL AZIZ ISMAIL³, HAZRINA HAZNI^2,4, KHADHER AHMAD¹, MUNIRAH ABD RAZZAK¹, ZULKIFLI MOHD YUSOFF¹ & KHALIJAH AWANG^2,4

¹Department of Al-Quran and Al-Hadith, Academy of Islamic Studies, University of Malaya, 50603 Kuala Lumpur, Federal Territory, Malaysia

²Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Federal Territory, Malaysia

³Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Malaya, 50603 Kuala Lumpur, Federal Territory, Malaysia

⁴Centre for Natural Products Research and Drug Discovery (CENAR), University of Malaya, 50603 Kuala Lumpur, Federal Territory, Malaysia

Received: 25 October 2019/Accepted: 7 March 2020

ABSTRACT

Lawsonia inermis (Henna), (Lythraceae), contains a high amount of phenolic compounds which could activate antioxidants to help reduce free radicals. In this study, the compound content found in the ethanol:water (80:20) extract of local Lawsonia inermis was determined using HPLC-QTOFMS. The ¹H-NMR results were used to determine the peak that exists for the group compounds. Chromatographic peaks were detected and integrated by the MassHunter Acquisition B.07.00 for the Agilent TOF and QTOF and MassHunter Qualitative Analysis B.07.00. The ethanol:water (80:20) extract of L. inermis have shown, predominantly, the presence of phenolic compounds (coumarins, flavonoids, naphthalene, and gallic acid) which are highly glycosylated. The presence of compounds such as apiin, lawsone, apigenin, luteolin, cosmosiin, and p-coumaric acid were also found.

Keywords: Ethanol:water (80:20); ¹H-NMR; HPLC-QTOFMS; leaves Lawsonia inermis  Malaysia

ABSTRAK

Lawsonia inermis (inai), (Lythraceae), mengandungi sejumlah besar sebatian fenolik yang boleh mengaktifkan antioksidan dalam membantu mengurangkan radikal bebas. Dalam kajian ini, kandungan sebatian yang terdapat dalam etanol:air (80:20) ekstrak Lawsonia inermis tempatan ditentukan menggunakan HPLC-QTOFMS. Hasil ¹H-NMR digunakan untuk menentukan puncak kumpulan sebatian. Puncak kromatografi dikesan dan diintegrasikan oleh MassHunter Acquisition B.07.00 untuk Agilent TOF dan QTOF dan Analisis Kualitatif MassHunter B.07.00. Daripada etanol:air (80:20) ekstrak L. inermis menunjukkan sebahagian besarnya, kehadiran sebatian fenolik (kumarin, flavonoid, naftalena dan asid galik) yang sangat glikosilasi. Kehadiran sebatian seperti apiin, lawson, apigenin, luteolin, kosmosiin dan asid-p-kumarit turut dijumpai.

Kata kunci: Daun Lawsonia inermis Malaysia; etanol:air (80:20); ¹H-NMR; HPLC-QTOFMS

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*Corresponding author; email: remy_um86@siswa.um.edu.my