Malaysian
Journal of Analytical Sciences Vol 19 No 1 (2015): 173 178
INTERACTION OF HYALURONIC ACID
(HA) WITH DIPALMITOYLPHOSPHATIDYLCHOLINE (DPPC) AND ITS EFFECT ON THE STABILITY
OF HA-LIPID TO GAMMA IRRADIATION
(Interaksi Asid Hyaluronik (HA) dan
Dipalmitoilfosfatidilkolina (DPPC) dan Kesan Penyinaran Gama ke atas Kestabilan
HA-Lipid)
Ainee
Fatimah Ahmad, Irman Abdul Rahman*, Hur Munawar Kabir Mohd, Faizal Mohamed,
Shahidan Radiman, Muhamad Samudi Yasir
School of Applied Physics,
Faculty of Science and Technology,
Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
*Corresponding author: irman@ukm.edu.my
Abstract
DPPC lipids are
extensively utilized as a biomembrane model as they resembled biological cells and their significance in various physiological functions
particularly in drug delivery system. The synovial
joint fluid containing hyaluronic acid, proteins, proteoglycans and lipids.
Hyaluronic acid (HA) is the most signified constituent in the synovial joint
fluid and functions as lubricant, nutrient carrier and shock absorber. Gamma
irradiation has also been discovered to be effeciently in depolymerizing and
cleaving molecular chains which initiated changes in chemical composition as
well as its physiological functions due to free radical. This research are
conducted to investigate the hyaluronic acid (HA) with 1,2 -dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC)
interaction in form of vesicles and its effect on the stability of HA-DPPC
structure to gamma radiation. The size distribution of DPPC vesicles measured
by Dynamic Light Scattering (DLS) is between 100 to 200 nm in diameter. HA was
added into the vesicles and characterized by using TEM to determine vesicle
size distributions, fusion and rupture of HA-DPPC structure. The results
demonstrated that the size of the vesicles approximately between 200 to 400 nm
which caused by vesicles fusion with HA and formed even larger vesicles. After
being irradiated by 0 to 200 Gy, the Z-average of DPPC vesicles decreased to
164.7 nm meanwhile for DPPC in presence of HA, the Z-average is 391.6 nm. FTIR spectra
were carried out to clarified formation of double bonds at ~1700-1750 cm-1
which leads to formation of pyrancarboxylic acid rings and modified the
structure of HA, hence its effect the structure of the DPPC vesicles.
Keywords: hyaluronic acid (HA),
dipalmitoylphosphatidylcholine (DPPC), gamma irradiation
Abstrak
Lipid
DPPC digunakan secara meluas sebagai model biomembran kerana mereka menyerupai
sel-sel biologi dan kepentingannya dalam pelbagai fungsi fisiologi terutamanya
dalam sistem penyampaian ubat. Cecair sendi sinovial mengandungi asid
hyaluronik, protein, proteoglikan dan lipid. Asid hyaluronik (HA) adalah unsur
yang penting di dalam cecair sendi sinovial dan berfungsi sebagai pelincir,
pembawa nutrien dan penyerap hentakan. Sinaran gama telah ditemui sangat
berkesan dalam dipolimerasikan dan memutuskan rantaian molekular yang telah
menyebabkan perubahan dalam komposisi kimia serta fungsi fisiologi
disebabkan radikal bebas. Kajian ini
dijalankan untuk mengkaji interaksi asid hyaluronik (HA) dengan
1,2-dipalmitoil-sn-glicero-3-fosfatidilkolina (DPPC) dalam bentuk vesikel dan
kesannya terhadap kestabilan struktur HA-DPPC terhadap sinaran gamma. Saiz
vesikel DPPC adalah antara 100 hingga 200 nm diameter diukur dengan menggunakan
Serakan Cahaya Dinamik (DLS). HA telah dicampurkan ke dalam vesikel dan
dicirikan dengan menggunakan TEM untuk menentukan taburan saiz vesikel,
gabungan dan pecahan struktur HA-DPPC. Hasil kajian menunjukkan bahawa saiz
vesikel kira-kira antara 200 hingga 300 nm yang disebabkan oleh gabungan
vesikel dan HA membentuk vesikel yang lebih besar. Setelah disinarkan pada
0-200 Gy, purata-Z vesikel DPPC menurun kepada 164.7 nm manakala untuk DPPC
dalam kehadiran HA, purata-Z adalah 391.6 nm. Spektra FTIR telah dijalankan
untuk menjelaskan pembentukan ikatan berganda pada ~1700-1750 cm-1 membawa kepada
perubahan pembentukan cincin asid pyrancarboxylic acid dan mengubah struktur
HA, oleh itu, struktur DPPC vesikel turut berubah.
Kata kunci: asid
hyaluronik, dipalmitoilfosfatidilkolina (DPPC), sinar gama
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