Malaysian Journal of Analytical Sciences Vol 19 No 2 (2015): 369 – 376

 

 

 

BIOMIMETIC SYNTHESIS OF SILVER NANOPARTICLES USING THE LICHEN Ramalina dumeticola AND THE ANTIBACTERIAL ACTIVITY

 

(Sintesis Biomimetik Nanozarah Perak Menggunakan Liken Ramalina dumeticola dan Aktiviti Antibakteria)

 

Laily B Din¹*, Ropisah Mie^1,3, Mohd Wahid Samsudin¹, Azizan Ahmad¹, Nazlina Ibrahim²

 

¹School of Chemical Sciences and Food Technology,

²School of Biosciences and Biotechnology,

Faculty of Science and Technology,

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

³Faculty of Applied Sciences,

Universiti Teknologi MARA, Negeri Sembilan Branch,

72000 Kuala Pilah, Negeri Sembilan, Malaysia

 

*Corresponding author: lbdin@ukm.edu.my

 

 

Received: 6 January 2015; Accepted: 10 February 2015

 

 

Abstract

Silver nanoparticles (AgNPs) has been successfully synthesized by reduction of silver nitrate with an aqueous extract of the lichen Ramalina dumeticola. The aqueous extract of Ramalina dumeticola was treated with 45 mL of 1 mM silver nitrate at room temperature (24-25 °C) for 24 hours. The ultraviolet-visible (UV-Vis) absorption spectroscopy has been used to monitor the formation of AgNPs. Physical appearance of AgNPs characterized by transmission electron microscopy (TEM) showed formation of AgNPs with average particle size of 13 nm. X-ray diffraction (XRD) techniques displayed the AgNPs as cubic structure. The UV-Vis absorption spectroscopy results showed a strong resonance centered on the surface of AgNPs at approximately 433 nm. The in vitro antibacterial activity of the synthesized AgNPs was investigated against eight bacterial strains using a disc diffusion method and it’s showed inhibition against all of them. The results revealed that the above AgNPs have potential as antibacterial agent.

 

Keywords: lichen extract, Ramalina dumeticola, silver nanoparticles, antibacterial activity

 

Abstrak

Nanozarah perak (NZAg) berjaya disintesis melalui penurunan argentum nitrat dengan ekstrak akueus liken Ramalina dumeticola. Ekstrak akueus Ramalina dumeticola diolah dengan 45 mL larutan argentum nitrat 1 mM pada suhu bilik (24-25 °C) selama 24 jam. Spektroskopi penyerapan ultraungu-boleh nampak (UU-BN) digunakan untuk mengawasi pembentukan NZAg. Penampakan fizikal NZAg dicirikan dengan mikroskopi transmisi elektron (MTE) yang menunjukkan pembentukan NZAg pada saiz zarah purata 13 nm. Teknik pembelauan sinar-X (PSX) memperlihatkan NZAg sebagai struktur kubik. Hasil spekroskopi penyerapan UU-BN menunjukkan resonans kuat yang berpusat pada permukaan NZAg pada kira-kira 433 nm. Aktiviti antibakteria in-vitro NZAg yang disintesis disiasat melawan lapan strain bakteria menggunakan kaedah peresapan cakera dan ia menunjukkan perencatan terhadap kesemuanya. Hasil ini mendedahkan bahawa NZAg di atas mempunyai potensi sebagai agen antibakteria.

 

Kata kunci: ekstrak liken, Ramalina dumeticola, nanozarah perak, aktiviti antibakteria

 

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