Malaysian Journal of Analytical Sciences Vol 19 No 2
(2015): 377 – 387
AN INVESTIGATION OF
POTENTIAL FRAUD IN COMMERCIAL ORANGE JUICE PRODUCTS IN MALAYSIAN MARKET BY
CLUSTER ANALYSIS AND PRINCIPAL COMPONENT ANALYSIS
(Satu Siasatan
Tentang Penipuan Berpotensi Dalam Produk Komersil Jus Oren Di Pasaran Malaysia
Dengan Analisis Kelompok Dan Analisis Komponen Utama)
Seow Eng Keng1,
Abbas Fadhl Mubarek Al-Karkhi2, Mohd Khairuddin Mohd Talib3,
Azhar Mat Easa1,
Cheng Lai Hoong1*
1Food Technology Division,
2Environmental Technology Division,
School of
Industrial Technology,
Universiti Sains
Malaysia, 11800 USM, Penang, Malaysia.
3Food Safety and Quality Division,
Kedah State
Health Department,
Lebuhraya
Sultanah Bahiyah, 05350 Alor Setar, Kedah, Malaysia
*Corresponding author: lhcheng@usm.my
Received: 14
January 2015; Accepted: 10 February 2015
Abstract
This
study was triggered by Malaysia Ministry of Health to monitor quality of
commercial orange juice products sold in Malaysia market. A total of 19 orange
juice samples from 14 different brands of packed orange juice products and 5
different brands of fresh orange fruit juices were analyzed for total soluble
solids content, total titratable acidity, sugar composition and amino acid
profiles. Hierarchical Cluster analysis (HCA) and Principal component analysis
(PCA) on amino acid composition alone allowed visual discrimination between
fresh squeezed orange juices and commercial packed orange juices. Suspicion of
mislabel was raised in cases of miss-classification.
Keywords: food
authentication, orange juice, principal component analysis, hierarchical
cluster analysis
Abstrak
Kajian ini dicetuskan oleh Kementerian
Kesihatan Malaysia demi memantau kualiti produk komersil jus oren yang dijual
dalam pasaran Malaysia. Sejumlah 19 sampel jus oren terbungkus dari pelbagai
jenama dan 5 jenama jus oren buah segar dari jenama berbeza telah dianalisa
dari segi kandungan zat terlarut, asiditi tertitrat, komposisi gula dan profil
asid amino. Analisis Kelompok Hierarki (HCA) dan Analisis Komponen Utama (PCA)
ke atas komposisi asid amino sahaja telah membenarkan pembezaan yang jelas
antara jus oren diperah segar dan jus oren komersil terbungkus. Kesangsian
silap-label telah dibangkitkan dalam kes-kes di mana kesilapan-klasifikasi
dapat dikesan
Kata kunci: Ketulenan
makanan, jus oren, analisis komponen utama, analisis kelompok hierarki
References
1.
Simpkins,
W.A. & Harrison, M. (1995). The state of the art in authenticity testing. Trends in Food Science & Technology, 6:321-328.
2.
Henshall,
A. A liquid chromatographic techniques for detecting economic aldulteration of
foods. Cereal Foods World, 43:98-103
3.
Saavedra,
L., García, Barbas, C. (2000). Development and validation of a capillary
electrophoresis method for direct measurement of isocitric, citric, tartaric
and malic acids as adulteration markers in orange juice. Journal of Chromatography A, 881:395-401.
4.
Reid, L.M., P. O’Donnell, C., Downey, G.
(2006). Recent technological advances for the determination of food
authenticity. Trends in Food Science and
Technology, 17:344-353.
5.
Robards,
K. & Antolovic, M. (1995). Methods for assessing the authenticity of orange
juice. Analyst, 120:1-8.
6.
Goodacre, R., Hammond, D., Kell, D.B. (1997).
Quantitative analysis of the adulteration of orange juice with sucrose using
pyrolysis mass spectrometry and chemometrics. Journal of Analytical and Applied Pyrolysis, 40-41:135-158.
7.
Simpkin,
W.A., Patel, G., Harrison, M. & Goldberg, D. (2000). Stable carbon isotope
ratio analysis of Australian orange juices. Food
Chemistry, 70:385-390.
8.
Ježek,
J. & Suhaj, M. (2001). Application of capillary isotachophoresis for fruit
juice authentication. Journal of
Chromatography A, 916, 185-189.
9.
Gόmez-Ariza,J.L., Villegas-Portero, M.J.
and Bernal-Daza, V. (2005). Characterization and analysis of amino acids in
orange juice by HPLC-MS/MS for authenticity assessment. Analytica Chimica Acta, 540:221-230.
10.
A.O.A.C. (1984) Official Methods of the
Association of Official Analytical Chemist. Kenneth Helrich, Arlington.
11.
Fry,
J. (1990) Authentication of orange juice. In: Production and Package of Non-carbonated Fruit Juices and Fruit
Beverage (Hicks D., ed.) Edinburgh: Blackie and Son Ltd., pp. 68-106.
12. McLellan, M.R.,
and Race, E.J. (1990). Grape juice processing. In: Production and Package of Non-carbonated Fruit Juices and Fruit
Beverage (Hicks D., ed.) Edinburgh: Blackie and Son Ltd., pp. 226-242.