Malaysian Journal of Analytical Sciences Vol 19 No 2 (2015): 402 – 405

 

 

 

EFFECT OF RADIATION ON THE VIABILITY OF HepG2 CANCER CELL LINE TARGETED WITH DIFFERENT AMOUNT OF RADIOSENSITIZER

 

(Kesan Sinaran Terhadap Kemandirian Sel Kanser HepG2 Yang Disasar Dengan Pelbagai Isipadu Pemeka Sinaran)

Cheong Kai Heng, Faizal Mohamed*,  Irman Abdul Rahman

 

School of Applied Physics, Faculty Science and Technology,

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

 

*Corresponding author: faizalm@ukm.edu.my

 

 

Received: 8 December 2014; Accepted: 14 January 2015

 

 

Abstract

Radiosensitizer (RS) were applied prior to radiation therapy to increase the therapeutic efficacy. This is due to some cancer cells were resistance toward radiation by producing large amount of antioxidant enzyme to scavenges the free radicals. The amount of RS added prior to irradiation played an important role to increase the efficacy of treatment but peroxide may cause unnecessary stress to the cancer cells before the treatment of irradiation. In this paper, different amount of RS were tested to evaluate the optimum amount of RS to avoid over-stress to the cancer cells while showing radiosensitizing effect by comparing to sample without RS. RS with different concentration started from 5% to 50% are added to hepatocellular carcinoma (HepG2) cancer cell line prior to 2Gy of fractionated dose. Cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 24 hours after irradiation. In this study it is observed that the concentration of RS range between 5-20% did not cause reduction on the viability percentage of the studied HepG2 cells whereas 25-50% caused significant reduction on cell viability. The radiosensitizing effect of RS at 20% concentration exhibits 18.68% more cell death compared to treatment with radiation alone. It is therefore suggested that 20% is the optimum amount of RS which able to enhance the radiosensitizing ability without causing toxicity to the cell, thus induced more cancer cell death during radiotherapy.

 

Keywords: Radiosensitizer, hyaluronan, radiotherapy, HepG2, MTT assay

 

Abstrak

Pemeka Sinaran (RS) diaplikasi sebelum terapi sinaran untuk meningkatkan keberkesanan rawatan kanser melalui kaedah sinaran. Pemeka sinaran digunakan kerana sebahagian sel kanser bersifat rintang terhadap sinaran yang disebabkan penghasilan enzim antioksida yang banyak lalu menghalang radikal bebas mendekati sel kanser. Penambahan jumlah RS sebelum penyinaran memainkan peranan penting untuk meningkatkan keberkesanan rawatan radioterapi tetapi peroksida boleh membawa tekanan yang keterlaluan ke atas sel  sebelum rawatan penyinaran. Dalam kajian ini, pelbagai kepekatan RS telah digunakan untuk menentukan kuantiti optimum bagi mengelakkan kesan keterlaluan RS ke atas sel kanser. Kepekatan RS dalam julat 5% hingga 50% disasarkan ke atas sel kanser HepG2 sebelum dos sebanyak 2Gy diberikan. Kemandirian sel diuji 24 jam selepas penyinaran dengan 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide asai MTT. Kajian ini mendapati bahawa kuantiti RS dari 5% ke 20% tanpa disinar menunjukkan tiada pengurangan dari segi peratusan kemandirian sel HepG2 manakala RS 25-50% menunjukkan pengurangan peratusan kemandirian sel HepG2 yang ketara. Kesan kepekaan RS pada 20% berupaya mengaruh 18.68% lebih kematian sel berbanding dengan rawatan sinaran tanpa RS. Kesimpulannya, kuantiti RS pada 20% adalah optimum dalam meningkatkan kesan kepekaan sinaran tanpa ketoksikan ke atas sel dan mengaruh lebih banyak kematian sel kanser ketika radioterapi dijalankan.

 

Kata kunci: Pemeka sinaran, hyaluronan, radioterapi, HepG2, asai MTT

 

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