Malaysian Journal of Analytical Sciences Vol 19 No 4
(2015): 904 - 910
IDENTIFICATION
AND OPTIMAL GROWTH CONDITIONS OF ACTINOMYCETES ISOLATED FROM MANGROVE
ENVIRONMENT
(Pengenalpastian
dan Pertumbuhan Optimum Aktinomiset dari Paya Bakau)
Ameerah Abdul Hamid,
Suhaidi Ariffin, Sharifah Aminah Syed Mohamad*
Faculty of Applied Sciences,
Universiti Teknologi MARA, 40450 Shah Alam, Selangor,
Malaysia
*Corresponding author: sharifah459@salam.uitm.edu.my
Received:
23 November 2014; Accepted: 27 June 2015
Abstract
Actinomycetes
are classified as saprophytes that play a significant role in the breakdown of
organic matters into more readily assimilable nutrients. They are also known to
produce a variety of secondary metabolites, which are useful for human, veterinary
medicine and agriculture applications. The optimum growth conditions of
actinomycetes isolated from mangrove environment at different temperature, pH
and sodium chloride (NaCl) concentration were studied. The identification of
the isolates by both biochemical test and 16S rRNA analysis was also done. All
isolates were grown in ISP1 medium at different temperature (25, 30, 37 and
40°C); pH (3.5, 5, 7 and 9); and salt concentration (0, 3, 5 and 7% w/v). The
optimum temperature for KMS1 and KMH2 isolates was at 25°C. However, the
optimum temperature for PCS1 and PMH1A was at 30 and 37°C respectively. The
optimum pH was pH7 and the most preferred NaCl concentration of all isolates
was at 3% (w/v). All isolates showed negative result for carbohydrate fermentation
except for KMS1 which showed utilisation of D-Fructose sugar with acid
production. Only KMS1 and PCS1 exhibited amylolytic and proteolytic activity.
The isolates were successfully identified as Streptomyces variabilis (KMS1),
Streptomyces rubrolavendulae (KMH2),
Streptomyces spp. CPE333 (PCS1) and Actinomycetales
bacterium (PMH1A).
Keywords: 16S rRNA analysis, biochemical test, growth conditions, marine
actinomycetes
Abstrak
Aktinomiset
boleh dikelaskan sebagai saprofit yang memainkan peranan utama di dalam
penguraian bahan organik kepada nutrien yang lebih mudah digunakan. Ia juga
diketahui boleh menghasilkan beberapa metabolit sekunder yang berguna untuk
manusia, perubatan veterinary dan aplikasi pertanian. Keadaan tumbesaran optima
untuk aktinomiset yang dipencilkan daripada persekitaran paya bakau pada suhu,
pH dan kepekatan natrium klorida (NaCl) yang berbeza adalah dikaji. Selain
daripada itu, pengenalpastian aktinomiset menggunakan kedua-dua ujian biokimia
dan analisa 16S rRNA juga dilakukan. Semua aktinomiset ditumbuhkan didalam ISP1
media pada keadaan yang berbeza iaitu suhu (25, 30, 37 dan 40°C); pH (3.5, 5, 7
dan 9) dan kepekatan garam (0, 3, 5 dan 7% w/v) untuk menentukan keadaan
pertumbuhan yang optimum. Suhu optimum untuk KMS1 dan KMH2 ialah pada 25°C.
Walau bagaimanapun, suhu optimum untuk PCS1 dan PMH1A adalah masing-masing pada
30 dan 37°C. pH optimum dan kepekatan NaCl untuk semua aktinomiset adalah
masing-masing pada pH 7 dan 3% (w/v). Semua aktinomiset menunjukkan keputusan
negatif ke atas penguraian karbohidrat kecuali KMS1 menunjukkan penggunaan gula
D-fruktosa dengan penghasilan asid. Hanya KMS1 dan PCS1 menunjukkan aktiviti
amilolitik dan proteolitik. Semua pencilan telah berjaya dikenal pasti sebagai Steptomyces
varibilis (KMS1), Streptomyces rubrolavendulae (KMH2), Streptomyces
spp. CPE333 (PCS1) dan Actinomycetales bacterium (PMH1A).
Kata kunci: 16S rRNA analisis, ujian biokimia, keadaan pertumbuhan, aktinomiset
marin
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