Malaysian Journal of Analytical Sciences Vol 23 No 4 (2019): 748 – 762

PSYCHROTOLERANT ACTINOBACTERIA Barrientosiimonas humi 39^T AS A SOURCE OF DIKETOPIPERAZINE

(Aktinobakteria Psikrotoleran Barrientosiimonas humi 39^T Sebagai Sumber Sebatian Diketopiperazin)

Andi Rifki Rosandy¹, Yeoh Chiann Ying², Cheah Yoke Kqueen², Muntaz Abu Bakar¹, Azira Muhamad³, Rozida Mohd Khalid¹*

¹School of Chemical Science and Food Technology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

²Department of Biomedical Sciences, Faculty of Medicine and Health Sciences

Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

³Malaysia Genome Institute (MGI),

National Institute of Biotechnology Malaysia (NIBM), Jalan Bangi, 43000 Kajang, Selangor, Malaysia

*Corresponding author: rozidakhalid@ukm.edu.my

Received: 31 March 2018; Accepted: 17 April 2019

Abstract

Barrientosiimonas humi 39^T (B. humi) is a psychrotolerant actinobacteria from the family Dermacoccaceae, was first isolated from Antarctica. Five diketopiperazine derivatives: cyclo(-Pro-Val) (1), (-)-cyclo(-Pro-Tyr) (2), cyclo(-Pro-Phe) (3), (+)-cyclo(-Pro-Leu) (4), and L-tyrosine (5) were isolated from the crude extract of B. humi, and this is the first reported compounds isolated from B. humi. The crude extracts were obtained via liquid-liquid fractionation using ethyl acetate as the organic solvent. The compounds were isolated and purified using chromatographic methods such as vacuum liquid chromatography and radial chromatography. The chemical structures were elucidated by nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry.

Keywords: Barrientosiimonas humi 39^T, diketopiperazine, Dermacoccaceae

Abstrak

Barrientosiimonas humi 39^T (B. humi) merupakan aktinobakteria psikrotoleran termasuk dalam famili Dermacoccaceae, asalnya dipencilkan dari Antartika. Lima terbitan diketopiperazin; siklo(-Pro-Val) (1), (-)-siklo(-Pro-Tir) (2), (-)-siklo(-Pro-Phe) (3), (+)-siklo(-Pro-Leu) (4) dan L-tirosina (5) diasingkan daripada ekstrak mentah B. humi, dan ini merupakan laporan pertama sebatian dipencilkan dari B. humi. Ekstrak mentah diperolehi melalui fraksi cecair-cecair menggunakan etil asetat sebagai pelarut. Sebatian-sebatian dipencilkan dan ditulenkan menggunakan kaedah kromatografi seperti kromatografi cecair vakum dan kromatografi jejari. Struktur kimia ditentukan menggunakan spektroskopi resonans magnetik nuklear (RMN) dan spektrometri jisim.

Kata kunci: Barrientosiimonas humi 39^T, diketopiperazine, Dermacoccaceae

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