Exploring the Long Non-Coding RNA, LOC285629 in Colorectal Cancer Cell Lines

By: Siti Nurmi Nasir, Science Officer C41 (ctnurminasir@ppukm.ukm.edu.my)

sw480 cellsColorectal cancer (CRC) is the most common type of cancer in the world. Despite its commonness, the underlying mechanism of CRC is not completely understood. Long noncoding RNAs (lncRNAs) have received increased attention with the development of whole genome and transcriptome sequencing technologies. Recent findings reveal that lncRNAs are implicated in serial steps of cancer development. These lncRNAs interact with DNA, RNA, protein molecules and/or their combinations, acting as an essential regulator in chromatin organization, and transcriptional and post-transcriptional regulation. In our study, we choose long non coding RNA, LOC285629 based on the in silico analysis of The Cancer Genome Atlas (TCGA) data results. The LOC285629 gene is an uncharacterized long non coding RNA and located in chr5q34. Little is known concerning the potential role of LOC285629 in the development and progression of CRC. Therefore we aimed to validate LOC285629 expression and to identify its function in the colorectal cancer model. The expression levels of LOC285629 in six human colorectal cancer cell lines (HT-29, SW 480, SW 48, CCL 220, HCT 116, and COLO 205) and a normal colonic epithelial cell (CCD 112 CoN) were screened using RT-qPCR.  The function of the lncRNA was determined by performing cell viability assay, colony formation, apoptosis assay, migration and invasion assay. LOC285629 expression levels were upregulated in SW 480 cell line therefore we chose the SW 480 cell lines for further studies. Our preliminary result showed that the knockdown of LOC285629 in SW 480 has significant effect on cell viability but not in the colony formation. In wound healing assay, the knockdown showed a slower growth on the cell covered surface area when compare to the control and non target. Other functional assays i.e colony formation, apoptosis assay, cell cycle assay, migration and invasion are currently ongoing.

The LOC 285629 expression were assessed by RT-qPCR in colorectal cancer cell lines (HT-29, SW 480, SW 48, CCL 220, HCT 116, and COLO 205) and was compared with the normal colonic epithelial cell (CCD 112 CoN).

The LOC 285629 expression were assessed by RT-qPCR in colorectal cancer cell lines (HT-29, SW 480, SW 48, CCL 220, HCT 116, and COLO 205) and was compared with the normal colonic epithelial cell (CCD 112 CoN).

Acknowledgement: This study is funded by Geran Universiti Penyelidikan (GUP-2014-068)