Sains Malaysiana 47(3)(2018): 427–432

http://dx.doi.org/10.17576/jsm-2018-4703-01

Testis Maturation Stages of Mud Crab (Scylla olivacea) Broodstock on Different Diets

(Peringkat Kematangan Testis Ketam Bakau (Scylla olivacea) Induk ke atas Diet yang Berlainan)

MHD IKHWANUDDIN^1*, GHAZALI AZMIE¹, SITI FATIMAH NAHAR², WENDY WEE², MOHAMAD N AZRA¹  & AMBOK-BOLONG ABOL-MUNAFI²

¹Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu Darul Iman, Malaysia

²School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu Darul Iman, Malaysia

Received: 1 August 2016/Accepted: 25 September 2017

ABSTRACT

The aim of this study was to investigate how various diets influence testis maturation stages in mud crab (Scylla olivacea) broodstock. Morphological and histological assessments were performed in triplicate (10 male crabs each). Daily, subject crabs were fed a squid (Loligo sp.) and a fish (Decapterus sp.) diet at 5-10% of body weight. Diets were analyzed following methods from the Association of Analytical Communities (AOAC). In comparison to control (wild) crabs, the two diets generally did not cause significant differences (p>0.05) in body weight, carapace width and gonadosomatic index (GSI), except in the GSI of squid-fed crabs (p<0.05). At the end of the experiment, crabs that reached Stage 3 testis maturation included were 6 fish-fed individuals and 23 squid-fed individuals. Additionally, differences in crude protein and fat levels across diets influenced the nature of male gonadal development. In conclusion, a squid diet was sufficient to induce Stage 3 testis maturation in Scylla olivacea within 60 days of culture. Our results should prove the usefulness in developing appropriate feeding regimes for male Scylla olivacea broodstock.

Keywords: Association of Analytical Communities; crab reproduction; crustacean; nutrition; testis maturation

ABSTRAK

Tujuan penyelidikan ini adalah untuk mengkaji bagaimana pelbagai jenis diet makanan mempengaruhi peringkat kematangan testis ketam bakau (Scylla olivacea) induk. Penilaian morfologi dan histologi dilakukan dalam tiga kali pengulangan (10 ekor induk jantan setiap kali pengulangan). Kesemua ketam diberi makan diet makanan sotong (Loligo sp.) dan ikan (Decapterus sp.) sebanyak 5-10% berat badannya setiap hari. Setiap diet makanan akan dianalisis mengikut kaedah daripada Association of Analytical Communities (AOAC). Secara amnya, jika dibandingkan dengan kumpulan ketam yang dikawal, kedua-dua jenis diet makanan tersebut tidak menunjukkan kesan yang signifikan (p>0.05) ke atas berat badan, saiz ukuran badan dan indeks kematangan gonadnya (GSI), kecuali indeks GSI ke atas ketam yang diberi makan sotong. Pada akhir kajian, hanya 6 ekor ketam yang diberi makan ikan dan 23 ekor ketam yang diberi makan sotong mencapai tahap 3 dalam peringkat kematangan testisnya. Sebagai tambahan, perbezaan antara protein mentah dan paras lemak dalam diet makanan telah mempengaruhi perkembangan gonad jantan secara semula jadi. Sebagai kesimpulan, diet makanan sotong sudah pun mencukupi untuk mendorong tahap 3 kematangan ketam jenis Scylla olivacea selama 60 hari ternakan. Keputusan kami adalah sangat berguna dalam membuktikan bahawa rejim pemakanan yang sesuai perlulah dibangunkan untuk induk jantan ketam Scylla olivacea.

Kata kunci: Association of Analytical Communities; kematangan testis; krustasia; nutrisi; pembiakan ketam

REFERENCES

Association of Analytical Communities (AOAC). 2002. Official Methods of Analysis. 17th ed. Washington DC: Association of Official Analytical Chemists.

Alava, V.R., Quinitio, E.T., dePedro, J.B., Priolo, F.M.P., Orozco, Z.G.A. & Wille, M. 2007. Lipids and fatty acids in wild and pond-reared mud crab Scylla serrata (Forsskal) during ovarian maturation and spawning. Aquaculture Research 38: 14681477.

Azra, M.N. & Ikhwanuddin, M. 2016. A review of maturation diets for mud crab genus Scylla broodstock: Present research, problems and future perspective. Saudi Journal of Biological Science 23: 257-267.

De-Lestang, S., Hall, N.G. & Potter, I.C. 2003. Reproductive biology of the blue swimmer crab (Portunus pelagicus, Decapoda: Portunidae) in five bodies of water on the west coast of Australia. Fishery Bulletin 101: 745-757.

Djunaidah, S., Wille, M., Kontara, E.K. & Sorgeloos, P. 2003. Reproductive performance and offspring quality in mud crab (Scylla paramamosain) broodstock fed different diets. Aquaculture International 11: 3-15.

Fazhan, H., Waiho, K., Wan Norfaizza, W.I., Megat, F.H. & Ikhwanuddin, M. 2017. Inter-species mating among mud crab genus Scylla in captivity. Aquaculture 471: 49-54.

Garcia, T.M. & Silva, J.R.F. 2006. Testis and vas deferens morphology of the red-clawed mangrove tree crab (Goniopsis cruentata) (Latreille, 1803). Brazilian Archives of Biology and Technology 49: 339-345.

Ikhwanuddin, M., Mohamed, S., Rahim, A.I.A., Azra, M.N., Jaaman, S.A., Bolong, A.M.A. & Noordin, N.M. 2015a. Observation on the effect of natural diets on ovarian rematuration in blue swimming crab Portunus pelagicus (Linnaeus, 1758). Indian Journal of Fisheries 62: 124-127.

Ikhwanuddin, M., Lan, S.S., Abdul-Hamid, N., Fatihah-Zakaria, S.N., Azra, M.N., Siti-Aisah, A. & Abol-Munafi, A.B. 2015b. The embryonic development of orange mud crab, Scylla olivacea (Herbst, 1796) held in captivity. Iranian Journal of Fisheries Sciences 14: 885-895.

Ikhwanuddin, M., Yin, T.P., Menon, A.J., Jasmani, S. & Abol- Munafi, A.B. 2015c. Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa. Sains Malaysiana 44(9): 1283-1288.

Ikhwanuddin, M., Liyana, A.N., Azra, M.N., Bachok, Z. & Abol- Munafi, A.B. 2014. Natural diet of blue swimming crab,  Portunus pelagicus at Strait of Tebrau, Johor, Malaysia. Sains Malaysiana 43(1): 37-44.

Keenan, C.P., Davie, P.J.F. & Mann, D. 1998. A revision of the genus Scylla de Haan, 1833 (Crustacea: Decapoda: Brachyura: Portunidae). Raffles Bulletin of Zoology 46: 217-245.

Leal, G.A., Dima, J.B., Dellatorre, F.G. & Baron, P.J. 2008. Schedule of reproductive events and maturity at size of the Patagonian stone crab Platyxanthus patagonicus (Brachyura, Platyxanthidae). Journal of Crustacean Biology 28: 262-269.

Marochi, M.Z., Moreto, T.F., Lacerda, M.B., Trevisan, A. & Masunari, S. 2013. Sexual maturity and reproductive period of the swimming blue crab Callinectes danae Smith, 1869 (Brachyura: Portunidae) from Guaratuba Bay, Parana State, Southern Brazil. Nauplius 21: 43-52.

Mura, M., Orru, F. & Cau, A. 2005. Size at sexual maturity of the spider crab, Anamathia rissoana (Decapoda: Majoidea) from the Sardinian Sea. Journal of Crustacean Biology 25: 110-115.

Millamena, O.M. & Quinitio, E. 2000. The effects of diets on reproductive performance of eyestalk ablated and intact mud crab Scylla serrata. Aquaculture 181: 81-90.

Millamena, O.M. 1989. Effect of fatty acid composition of broodstock diet on tissue fatty acid patterns and egg fertilization and hatching in pond-reared Penaeus monodon. Asian Fisheries Science 2: 127-134.

Robertson, W.D. & Kruger, A. 1994. Size at maturity, mating and spawning in the portunid crab Scylla serrata (Forskal) in Natal, South Africa. Estuarine, Coastal and Shelf Science 39: 185-200.

Ryan, E.P. 1967. Structure and function of the reproductive system of the crab Portunus sanguinolentus (Herbst) (Brachyura: Portunidae). I. Male system. Proceedings of a Symposium on Crustacean. The Marine Biological Association of India, Part II. pp. 506-521.

Soundarapandian, P., Varadharajan, D. & Boopathi, A. 2013. Reproductive biology of the commercially important portunid crab, Portunus sanguinolentus (Herbst). Journal of Marine Science: Research and Development 3: 1-9.

Viau, V.E., Greco, L.S.L., Bond-Buckup, G. & Rodriguez, E.M. 2006. Size at the onset of sexual maturity in the anomuran crab, Aegla uruguayana (Aeglidae). Acta Zoologica 87: 253-264.

Viswanathan, C. & Raffi, S.M. 2015. The natural diet of the mud crab Scylla olivacea (Herbst, 1896) in Pichavaram mangroves, India. Saudi Journal of Biological Sciences 22: 698-705.

Waiho, K., Fazhan, H., Jasmani, S. & Ikhwanuddin, M. 2017. Gonadal development in males of the orange mud crab, Scylla olivacea (herbst, 1796) (Crustacea: Decapoda: Brachyura: Portunidae). Crustaceana 90: 1-19.

Waiho, K., Fazhan, H., Baylon, J.C., Wan Norfaizza, W.I. & Ikhwanuddin, M. 2016a. Use of abdomen looseness as an indicator of sexual maturity in male mud crab Scylla spp. Journal of Shellfish Research 35: 1027-1035.

Waiho, K., Fazhan, H. & Ikhwanuddin, M. 2016b. Size distribution, length-weight relationship and size at the onset of sexual maturity of the orange mud crab, Scylla olivacea in Malaysian waters. Marine Biology Research 12: 726-738.

Waiho, K., Mustaqim, M., Fazhan, H., Wan Norfaizza, W.I., Megat, F.H. & Ikhwanuddin, M. 2015. Mating behaviour of the orange mud crab, Scylla olivacea: The effect of sex ratio and stocking density on mating success. Aquaculture Reports 2: 50-57.

*Corresponding author; email: ikhwanuddin@umt.edu.my