Sains Malaysiana 47(3)(2018): 517–522
http://dx.doi.org/10.17576/jsm-2018-4703-11
Kajian Dok Molekul Mengenai Interaksi antara RNA-Bergantung RNA Polimerase Virus
Denggi dan Analog Nukleosida
(Molecular Docking Study of the Interactions
between Dengue Virus RNA-Dependent-RNA
Polymerase and Nucleoside Analogues)
NOR NADIRAH ABDULLAH, KAMAL RULLAH, LAM KOK WAI
& MALINA JASAMAI*
Drugs & Herbal Research
Centre, Faculty
of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300
Kuala Lumpur, Wilayah Persekutuan, Malaysia
Received: 8 June 2017/Accepted:
24 October 2017
ABSTRAK
Enzim RNA-bergantung RNA polimerase adalah sasaran dadah yang menarik untuk mengubati
jangkitan denggi. Analog nukleosida menyerupai substrat asal enzim polimerase.
Ia bertindak sebagai perencat atau substrat kepada enzim ini lalu menyebabkan
penamatan pramatang bebenang DNA/RNA atau penghasilan DNA/RNA yang rosak. Ini akan menghentikan proses replikasi virus. Kajian
dok molekul untuk mengenal pasti interaksi molekular antara enzim dan ligannya
telah dilakukan berdasarkan maklumat yang diperoleh berkenaan struktur kristal
domain RdRp. Tapak pengikat-ligan domain RdRp yang terdiri daripada sisa asid amino
Asn492, Asn405, Lys401, Thr605 dan Gly601 telah dikenal pasti setelah
pengedokan analog nukleosida yang boleh didapati secara komersial dijalankan.
Pengedokan analog nukleosida yang menyerupai substrat asal RdRp ke dalam tapak
pengikat menunjukkan mod pengikat-ligan dengan ikatan hidrogen, aromatik-π dan
interaksi cas adalah interaksi utama yang terlibat. Kajian ini juga memberi
maklumat berkenaan farmakofor analog nukleosida yang boleh digunakan dalam
reka-bentuk dadah berasaskan struktur terhadap sasaran penting ini.
Kata kunci: Analog nukleosida; dok
molekul; enzim RdRp; tapak pengikat-ligan; virus denggi
ABSTRACT
RNA-dependent
RNA
polymerase (RdRp) enzyme is an attractive drug target
to treat dengue infection. Nucleoside analogues are mimics of natural
substrates for polymerase enzymes. They act as inhibitors or substrates
for these enzymes resulted in the premature termination of the DNA/RNA strands or formation of
faulty DNA/RNA strands. This will halt the virus replication process.
Based on the published crystal structure of RdRp domain, docking
study to identify molecular interactions between the enzyme and
its ligands were performed. Docking of the commercially available
nucleoside analogues identified the ligand-binding pocket of the
RdRp domain encompasses of Asn492, Asn405, Lys401, Thr605 and Gly601
amino acid residues. Docking of the nucleoside analogues, mimics
of the natural substrate for RdRp into this pocket showed the ligand-binding
mode, in which hydrogen bonding, π-aromatic and charge interactions
are the main forces involved. This study also showed the pharmacophore
of the nucleoside analogues which will be useful in structure-based
drug design against this important target.
Keywords: Dengue virus; ligand-binding pocket; molecular docking;
nucleoside analogues; RdRp enzyme
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*Corresponding author; email: malina@ukm.edu.my
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