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Sains Malaysiana 50(7)(2021): 1959-1970
http://doi.org/10.17576/jsm-2021-5007-11
Potential of Hibiscus sabdariffa Linn. Polyphenol-Rich Extract in Improving Diabetes-Induced Vascular Functional and Structural Abnormalities in Rats
(Potensi Ekstrak Hibiscus sabdariffa Linn. Kaya Polifenol Membaikpulih Ketaknormalan Fungsi dan Struktur Vaskular Aruhan Diabetis pada Tikus)
FATIN FARHANA
JUBAIDI1, SATIRAH ZAINALABIDIN2, LAI YING1,
NUR LIYANA MOHAMMED YUSOF1 & SITI BALKIS BUDIN1*
1Center
for Diagnostic, Therapeutic and Investigative Studies (CODTIS), Faculty of
Health Science, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Federal
Territory, Malaysia
2Centre
for Toxicology and Health Risk Studies (CORE), Faculty of Health Sciences, Universiti
Kebangsaan Malaysia, 50300, Kuala Lumpur, Federal Territory, Malaysia
Received: 14 September 2020/Accepted: 2
December 2020
ABSTRACT
Hibiscus sabdariffa Linn. contains a high concentration of
polyphenolic compounds and shows potentials in reducing vascular complications
in diabetes mellitus (DM) patients. This study was aimed to determine the ability of H. sabdariffa Linn. polyphenol-rich extract (HPE) in improving vascular
endothelial dysfunction and attenuating oxidative stress in type 1 DM. DM was
induced in adult male rats and the rats were then divided into three groups;
untreated DM (DM); DM with HPE supplementation (DM+HPE); and DM with metformin
(DM+MET). Another group of non-diabetic rats served as the normal control
group. These rats were left untreated for four weeks before being subjected to
the supplementations for another four weeks. The thoracic descending aorta was
isolated from the treated and untreated rats to measure the vascular
reactivity, oxidative stress, and morphological alterations. The results showed
that HPE supplementation significantly reduced the systolic blood pressure
(SBP) and mean arterial pressure (MAP) in the DM+HPE group (p<0.05). HPE
also showed a tendency to improve endothelium-dependent vasorelaxation compared
to the untreated diabetic rats. Rats treated with HPE exhibited a considerable
improvement in the activities of antioxidants and significantly attenuated
oxidative damage (p<0.05). Histological findings showed that HPE
supplementation improved morphological changes in the aorta. In conclusion, HPE
supplementation reduces vascular abnormalities in DM condition probably via amelioration of oxidative stress.
Keywords: Antioxidants;
contractile response; oxidative stress; relaxation response; roselle
ABSTRAK
Kata kunci: Antioksidan; rosel; tekanan oksidatif;
tindak balas mengecut; tindak balas relaksasi
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*Corresponding author; email: balkis@ukm.edu.my
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