Sains Malaysiana 50(7)(2021): 1947-1957

http://doi.org/10.17576/jsm-2021-5007-10

Production of Docosahexaenoic Acid, DHA using Different Modes of Cultivation by Aurantiochytrium sp. SW1

(Penghasilan Asid Dokosaheksaenoik, DHA menggunakan Mod Pengkulturan Berbeza oleh Aurantiochytrium sp. SW1)

SHARIFFAH NURHIDAYAH SYED ABDUL RAHMAN¹, MOHD SAHAID KALIL² & AIDIL ABDUL HAMID¹*

¹Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

²Department of Chemical and Processes Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

Received: 13 February 2020/Accepted: 16 November 2020

ABSTRACT

Thraustochytrids, such as members of the genus Aurantiochytrium, are rich in docosahexaenoic acid (DHA, C22:6n-3) and represent a promising source of omega-3 fatty acids which plays a vital role in the enhancement of human health, particularly for neurological and visual functions. Different modes of cultivation (batch, fed-batch and repeated-batch) by Aurantiochytrium sp. SW1 were studied for effective docosahexaenoic acid (DHA) production. In this study, three different modes of fermentation were carried out in 1 L shake flasks with a working volume of 500 mL, incubated at 30 ºC and 200 rpm. Batch cultivation significantly exceeds the rest of cultivation modes, achieving maximal lipid and DHA concentrations of 11.22 g/L and 5.87 g/L, respectively, and DHA productivity of 0.061 g/L/h. Lipid and DHA concentration of the repeated-batch process decreased through the cycles for all three different types of replacement ratio (80, 90 and 95%). The average decrease percentage of DHA concentration for cycle one and cycle two were 21.76 and 32.52%, respectively. However, the fatty acid composition of lipids obtained in the cycles remained consistent with 16:0 and DHA being the most abundant fatty acids indicating that this mode of fermentation is highly useable for industrial applications.

Keywords: Aurantiochytrium sp. SW1; docosahexaenoic acid; fed-batch; repeated-batch

ABSTRAK

Thraustochytrid, seperti dalam kumpulan genus Aurantiochytrium adalah kaya dengan asid dokosaheksaenoik (DHA, C22:6n-3) dan merupakan sumber asid lemak omega-3 yang memainkan peranan penting dalam memelihara kesihatan manusia terutamanya dalam fungsi neuron dan penglihatan. Penghasilan DHA oleh Aurantiochytrium sp. SW1 dalam mod pengkulturan berbeza (kultur kelompok, kultur suap-kelompok dan kultur kelompok ulangan) yang lebih berkesan diteliti. Semua mod pengkulturan ini dijalankan dalam kelalang goncangan 1 L, dengan 500 mL medium penghasilan pada 30 ºC dan 200 rpm. Mod pengkulturan kultur kelompok adalah lebih signifikan berbanding mod pengkulturan lain apabila menghasilkan kepekatan lipid dan DHA tertinggi dengan nilai masing-masing 11.22 dan 5.87 g/L, serta produktiviti DHA bersamaan 0.061 g/L/jam. Dalam kultur kelompok ulangan (isipadu tertentu medium dituai dan baki kultur ditambah dengan sejumlah medium baru), kepekatan lipid dan DHA didapati berkurangan daripada kitaran 1 sehingga memasuki kitaran 2 bagi ketiga-tiga medium nisbah gantian (80, 90 dan 95%). Peratus pengurangan bagi kepekatan DHA dalam kitaran 1 dan 2, masing-masing adalah 21.76 dan 32.52%. Walau bagaimanapun, ketekalan komposisi asid lemak pada nisbah 16:0 dan DHA merupakan asid lemak yang paling banyak diperoleh dalam dua kitaran pengkulturan, menunjukkan bahawa mod penapaian bagi kultur kelompok ulangan berpotensi tinggi bagi kegunaan industri.

Kata kunci: Asid dokosaheksaenoik; Aurantiochytrium sp. SW1; kultur kelompok-ulangan; kultur suap-kelompok

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*Corresponding author; email: aidilah@ukm.edu.my