Sains Malaysiana 51(11)(2022): 3731-3739

http://doi.org/10.17576/jsm-2022-5111-17

Determination of Non-Organ Specific Autoantibodies in Patients with Chronic Hepatitis C and Association with HLA DRΒ1 (*04) Allele

(Penentuan Autoantibodi Khusus Bukan Organ pada Pesakit Hepatitis C Kronik dan Perkaitan dengan Alel HLA DRΒ1 (*04))

FATIMA AYUB¹, ROMEEZA TAHIR^1,*, MUHAMMAD KASHIF^1,2, KHURSHEED JAVAID¹, FAHEEM SHAHZAD¹, SHAH JAHAN¹ & NADEEM AFZAL¹

¹Department of Immunology, University of Health Sciences, Khyaban-e-Jamia Punjab, 54000, Lahore, Pakistan

²Department of Oral Pathology, Bakhtawar Amin Medical & Dental College, Multan, Pakistan

Received: 24 May 2021/Accepted: 28 June 2022

Abstract

The regulation of immune mechanisms is controlled by major histocompatibility complex/human leukocyte antigen (MHC/HLA). Polymorphisms of the HLA region have an impact on susceptibility to complex infectious and autoimmune diseases. The present study was carried out to determine the frequencies of ASMA, AMA, ANA, dsDNA, and anti-LKM-1 auto-antibodies in hepatitis C patients and to determine their association with the HLA DRβ1 (*04) locus. It was a cross-sectional, analytical study, and 86 patients with chronic HCV were recruited. The presence of auto-antibodies (ASMA, AMA, ANA, dsDNA, and anti-LKM-1) was determined by indirect immunofluorescence and ELISA, while the HLA DRβ1 (*04) allele was assessed by sequence-specific conventional PCR. ANA was detected in 41%, ASMA in 17.4%, AMA in 7%, LKM-1 in 5.8% dsDNA in 4.6% of CHC patients while HLA-DRβ1 (*04) was present in 3.5% of patients, but this was not significantly associated with these auto-antibodies.

Keywords: Auto-antibodies; HCV; hepatitis C; HLA DRβ1; immunofluorescence

Abstrak

Pengawalaturan mekanisme imun dikawal oleh kompleks histoserasian utama/antigen leukosit manusia (MHC/HLA). Polimorfisme rantau HLA mempunyai kesan ke atas kerentanan kepada penyakit berjangkit dan autoimun yang kompleks. Kajian ini dijalankan untuk menentukan kekerapan ASMA, AMA, ANA, dsDNA dan anti-LKM-1 auto-antibodi pada pesakit hepatitis C dan untuk menentukan hubungan mereka dengan lokus HLA DRβ1 (*04). Ia adalah kajian keratan rentas, analisis dan 86 pesakit dengan HCV kronik telah diambil. Kehadiran auto-antibodi (ASMA, AMA, ANA, dsDNA dan anti-LKM-1) ditentukan oleh imunofluoresensi tidak langsung dan ELISA, manakala alel HLA DRβ1 (* 04) dinilai oleh urutan khusus PCR konvensional. ANA dikesan pada 41%, ASMA pada 17.4%, AMA pada 7%, LKM-1 dalam 5.8% dsDNA pada 4.6% pesakit CHC manakala HLA-DRβ1 (*04) hadir pada 3.5% pesakit, tetapi ini tidak secara signifikan dikaitkan dengan auto-antibodi ini.

Kata kunci: Auto-antibodi; HCV; hepatitis C; HLA DRβ1; imunopendafluor

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^*Corresponding author; email: romeeza.tahir@uhs.edu.pk