Sains Malaysiana 35(1): 7-10 (2006)

 

Transduser Optik Hidrogen Peroksida Berasaskan Enzim

Peroksidase Terpegun dalam Monolit Hibrid Sol-Gel-Kitosan

(Optical Transducer for Hydrogen Peroxide Based on Immobilised

Peroxidase Enzyme in Monolith of Sol-Gel-Chitosan Hybrid)

 

 

Nurul Izzah Ab Rahman & Musa Ahmad

Pusat Pengajian Sains Kimia & Teknologi Makanan

Fakulti Sains & Teknologi

Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor D.E. Malaysia

 

 

ABSTRAK

 

Kajian pemegunan enzim peroksidase dalam membran hibrid sol-gel-kitosan untuk penyediaan transduser optik hidrogen peroksida telah dilakukan dalam pembinaan biosensor. Kajian ini merangkumi pengoptimuman nisbah sol-gel-kitosan, pencirian enzim dalam larutan bebas dan pemegunan enzim dalam hibrid sol-gel-kitosan serta penciriannya. Spektrum serapan bagi setiap pencirian ditentukan menggunakan Spektrofotometer uL-Nampak. Hasil kajian menunjukkan aktiviti enzim tidak berubah selepas pemegunan. Nisbah optimum hibrid sol-gel-kitosan adalah 4:6. Nilai sisihan piawai relatif (RSD) untuk kebolehulangan kaedah ini adalah 2.71% dan 3.76%, masing-masingnya untuk enzim bebas dan enzim terpegun.

 

Kata kunci: Transduser optik, enzim peroksidase, hibrid sol-gel-kitosan, monolit, hidrogen peroksida

 

 

ABSTRACT

 

A study on the immobilisation of peroxidase enzyme in sol-gel-chitosan hybrid monolith for the preparation of hydrogen peroxide optical transducer has been conductedfor biosensor fabrication. This research involved the optimisation of the sol-gel-chitosan ratio, characterization of enzyme infree solution and immobilization of peroxidase enzyme in sol-gel­chitosan hybrid. The characterizations were studied by using uv- Visible Spectrophotometer. The results showed that the enzyme did not change its biological activities after immobilisation. The optimised ratio of sol-gel:chitosan was 4:6. The relative standard deviation (RSD) values for the reproducibility of the method based onfree and immobilized enzyme were 2.71% and 3.76%, respectively.

 

Keywords: Optical transducer, peroxidase enzyme, sol-gel-chitosan hybrid, monolith, hydrogen peroxide

 

 

RUJUKAN/REFERENCES

 

Badini, G.T., Grattan, K.T.V., Palmer, A.W. & Tseung, A.C.C. 1989. Development of pH sensitive substrate for optical sensor application. Springer Proceedings in Physics for 6th Int. Conf Optical Fibre Sensors. 44. H.J. Arditty, J.P. Dakin & R.T. Kersten (eds). Springer-Verlag. Berlin.

Kadnikova, E.N., & Kosti, N.M., 2002. Oxidation of ABTS by hydrogen eroxide catalyzed by horseradish peroxidase encapsulated into sol-gel glass. Journal of Molecular Catalysis B: Enzymatic 18(1-3): 39-48.

Musa Ahmad. 2003. Production of optical chemical sensing materials using sol-gel technique. Dlm.Che Husna Azhari, Andanastuti Muchtar & Ahmad Kamal Arifin Mohd. Ihsan (pnyt) Advances In Materials Processing, hlm. 36-61. Kuala Lumpur: Institute of Materials.

Putter. J, R. Becker (Eds). 1983. Peroxidase. Deerfield Beach: Verlag Chemie.

Wang, G., Xu, J.J, Chen, H.Y, & Lu, Z.H. 2003. Amperometric hydrogen peroxide biosensor in sol-gel/chitosan network-like film as immobilization matrix. Biosensor and Bioelectronics 18(4): 335-343.

 

 

 
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