Sains Malaysiana
37(4): 429-433(2008)
Peningkatan Produktiviti CGtase dengan Menggunakan
Kultur Selanjar
(Increased productivity of CGTase using continuous culture)
Khosiya Sali, Aidil Abdul Hamid & Wan Mohtar Wan
Yusoff
Program Mikrobiologi, Pusat pengajian Biosains dan Bioteknologi
Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia
43600 Bangi, Selangor, Malaysia
Diserahkan: 12 April 2007 / Diterima: 14 Disember 2008
ABSTRAK
Penghasilan
CGTase daripada Bacillus sp. G1 berjaya ditingkatkan
dengan menggunakan sistem kultur selanjar mengatasi penghasilan
daripada kultur kelompok. Aktiviti CGTase tertinggi yang
didapati dalam kultur kelompok ialah 28.1 U/ml. Kajian
kultur selanjar difokuskan kepada masa kemasukan medium
segar yang berbeza (12, 24 dan 48 jam ), kadar pencairan
ditetapkan pada 0.03 per jam. Hasil menunjukkan masa memulakan
pam medium segar tidak memberi perubahan yang signifikan
terhadap aktiviti CGTase (25.7, 26.3 dan 26.1 U/ml masing-masing)
dan produktiviti CGTase (0.77, 0.79 dan 0.78 U/ml/j masing-masing)
pada keadaan mantap tetapi produktiviti CGTase (0.77 U/ml/j)
akan lebih tinggi berbanding produktiviti kultur kelompok
apabila masa di antara larian kultur kelompok diambil kira.
Malah peningkatan berpotensi ditingkatkan lagi dengan memulakan
pam medium segar lebih awal daripada 12 jam dan juga dengan
meningkatkan kadar pencairan.
Kata
kunci: Bacillus sp. G1; CGTase; kultur selanjar
ABSTRACT
The
production of CGTase from Bacillus sp. G1 was successfully
increased using continuous culture. The highest CGTase
activity in batch culture was 28.1 U/ml. The study in continuous
culture was focused on the effect of different time (12,
24 and 48 hours) to start pumping fresh medium at fixed
dilution rate of 0.03 per hour. Results showed that the
pumping time did not give significant changes in CGTase
activity (25.7, 26.3 and 26.1 U/ml respectively) and in
CGTase productivity (0.77, 0.78 and 0.78 U/ml/h) respectively
at steady state operation. Nevertheless, the productivity
(0.77 U/ml/h) of CGTase was found to be higher than that
obtained in batch culture when the time interval between
batch run was taken into account. The productivity could
be further increased by pumping the media earlier than 12
hours and also by increasing the dilution rate.
Keyword:
Bacillus sp. G1; CGTase; continuous culture
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