Sains Malaysiana 38(3): 413-418(2009)

 

 

Pembangunan Sensor Optik Ph Berasaskan Reagen Kurkumin Terpegun

Dalam Matriks Hibrid Sol-Gel/Kitosan Melamin

(Development of optical pH sensor based on curcumin reagent immobilized

in sol-gel/chitosan melamine hybrid matrices)

 

 

Rosmawani Mohammad, Musa Ahmad* & Jamaluddin Mohd Daud

Pusat Pengajian Sains Kimia & Teknologi Makanan

Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia

43600 Bangi, Selangor DE., Malaysia

Diserahkan: 20 Jun 2008 / Diterima: 4 Ogos 2008

 

 

ABSTRAK

Sensor optik pH berasaskan reagen kurkumin terpegun dalam matriks hibrid sol-gel/kitosan melamin telah dibangunkan dalam kajian ini. Sensor optik pH ini memberikan rangsangan linear pada julat pH 9-13 (R2 = 0.9846). Seterusnya, nilai RSD kebolehulangan adalah  7.38% - 9.65%, manakala nilai RSD kebolehasilan pula adalah 2.18% - 4.72%. Kestabilanfoto yang baik bagi reagen kurkumin terpegun diperolehi apabila filem disimpan dalam persekitaran gelap dengan nilai RSD 3.69% untuk tempoh kajian 3 minggu. Tiada kesan histerisis diperhatikan apabila sensor digunakan untuk mengukur pH dalam kitaran pH 2-12-2. Masa rangsangan sensor adalah 10-20 saat. Penentuan nilai pH bagi beberapa sampel sebenar menggunakan sensor pH yang dihasilkan dalam kajian ini menunjukkan tiada perbezaan secara signifikan pada nilai pH yang diperolehi apabila dibandingkan dengan keputusan yang diperolehi menggunakan meter pH.

Kata kunci: Hibrid sol-gel/kitosan melamin; kurkumin; sensor optik pH  

ABSTRAK  

An optical pH sensor has been developed using curcumin reagent immobilized in sol-gel/chitosan-melamine hybrid matrices. The optical pH sensor gave a linear response at range of pH 9-13 (R2 = 0.9846). Relative  standard  deviation (RSD) values of  reproducibility were 7.38% - 9.65%, while RSD values of repeatability were 2.18% - 4.72%. Good photostability was obtained for immobilized curcumin reagent when the films were kept in dark surrounding with RSD value 3.69% for 3 weeks. No hysteresis effect was observed when the sensor was used to measure pH in cycle from pH 2-12-2. The response time of the sensor was within 10-20 s. Determination of pH value in real samples by using pH sensor developed in this study showed no significance differences when compared with results obtained by using conventional pH meter.

 

Keywords: Curcumin; hybrid sol-gel/chitosan-melamine; optical pH sensor  

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*Pengarang untuk surat-menyurat; email: andong@ukm.my

 

 

 

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