Sains Malaysiana 39(2)(2010): 161–168
Isolation and Characterization
of Bacteria Degrading Sumandak and South Angsi Oils
(Pemencilan dan Pencirian Bakteria Pendegradasi Minyak Sumandak dan South Angsi)
AINON HAMZAH*, AMIR RABU, RAJA FARZARUL HANIM RAJA AZMY
& NOOR AINNI YUSSOFF
School of Biosciences and Biotechnology
Faculty of Science and Technology
Universiti Kebangsaan Malaysia
43600 Bangi, Selangor D.E.
Malaysia
Diserahkan:
12 Mac 2009 / Diterima: 14 Oktober 2009
ABSTRACT
Four species of bacteria, Acinetobacter lwoffii, Aeromonas hydrophila,
Pseudomonas aeruginosa and Pseudomonas putida, were isolated from soil contaminated with
hydrocarbons and selected for the determination of growth requirements and the
ability to degrade petroleum hydrocarbon. The bacteria were grown in mineral
salt medium (MSM) supplemented
with two types of crude oil, either Sumandak or South Angsi at 1% (v/v) concentration. The optimum pH for
growth of A. hydrophila and P. aeruginosa was 6.5 when grown with Sumandak and South Angsi oil. For A. lwoffii and P. putida the optimum pH for growth
with Sumandak and South Angsi oil was 6.5 or 7.0, respectively. The growth of P. aeruginosa was the highest in MSM when
supplemented with 1% South Angsi oil and 0.5% tryptone at pH 6.5 while, in Sumandak oil the growth was the highest when yeast extract was added. Gas chromatography
analysis showed that the South Angsi crude oil
components of C12 to
C25 were more extensively
degraded by A. lwoffii after 24 h of incubation
compared to the other bacteria over the same period.
Keywords: Bacteria;
biodegradation; crude oil; petroleum hydrocarbon
ABSTRAK
Empat spesies bakteria, iaituAcinetobacter lwoffii, Aeromonas hydrophila, Pseudomonas aeruginosa dan Pseudomonas putida telah dipencilkan daripada tanah terlumus dengan hidrokarbon dan dipilih untuk penentuan keperluan pertumbuhan dan keupayaan untuk mendegradasi hidrokarbon petroleum. Bakteria dihidupkan di dalam medium garam mineral (MSM) yang dibekalkan dengan dua jenis minyak mentah, sama ada Sumandak atau South Angsi pada kepekatan 1% (i/i). pH optimum bagi pertumbuhan pencilanA. hydrophila and P. aeruginosa ialah 6.5 apabila dihidupkan dengan minyak Sumandak dan South Angsi. Bagi A. lwoffiidan P. putida pH optimum untuk pertumbuhan dengan minyak Sumandak dan South Angsi masing-masing adalah 6.5 dan 7.0. PertumbuhanP. aeruginosa adalah paling tinggi apabila dibekalkan dengan 1% minyak South Angsi dan 0.5% tripton pada pH 6.5 manakala dalam minyak Sumandak, pertumbuhan adalah tinggi apabila ditambah yis ekstrak. Analisis kromatografi gas menunjukkan komponen C12 sehingga C25 dalam minyak mentah South Angsi lebih banyak didegradasikan oleh A. lwoffii berbanding bakteria lain selepas 24 jam pengeraman.
Kata kunci: Bakteria; biodegradasi; hidrokarbon petroleum; minyak mentah
RUJUKAN
Adebusoye,
S.A., Ilori, M.O., Amund,
O.O., Teniola, O.D. & Olatope,
S.O. 2006. Microbial degradation of petroleum
hydrocarbons in a polluted tropical stream. Journal of American
Science 2(3): 48-56.
Ajisebutu, S.O. 1988. Effects
of sodium chloride on biodegradation of crude oils by two species of Aeromonas. Applied Microbiology and Biotechnology 28(2): 203-208.
Azmy,
R.F.H.R. & Hamzah, A. 2007. Optimization of growth by Pseudomonas sp. UKMP14T in
degrading Tapis crude oil. Proceedings of
the 29th Symposium of
Malaysian Society for Microbiology (E14)1-6.
Barkay,
T., Navon-Venezia, S., Ron, E.Z. & Rosenberg, E.
1999. Enhancement of solubilization and biodegradation of polyaromatic hydrocarbons by the bioemulsifier. Applied
Environmental Microbiology 65: 2697-702.
Balba,
M.T., al-Awadhi, N. & al-Daher, R. 1998. Bioremediation of oil-contaminated soil:microbiological method for feasibility assessment and field evaluation. Journal of
Microbiological Methods 32: 155-164.
Bouchez,
M., Blanchet, D. & Vandecasteele, J.P. 1995. Degradation of polycyclic aromatic hydrocarbon by pure strains and by defined
strain associations: inhibition phenomena cometabolism. Applied Microbiology Biotechnology 43: 156-164.
Breuil,
C., Shindler, D.B., Sijher,
J.S. & Kushner, D.J. 1978. Stimulation
of lipase production during bacterial growth on alkanes. Journal of Bacteriology 133(2): 601-606.
Chaillan, F., Fleche, A.L., Bury, E., Phantavong, Y., Grimont, P., Saliot, A. & Oudot, J. 2004. Identification and biodegradation potential of tropical aerobic
hydrocarbon-degrading microorganisms. Research in Microbiology 155:
587-595.
Chaineau, C.H., Morel, J., Dupont,
J., Bury, E. & Oudot, J. 1999. Comparison of the
fuel oil biodegradation potential of hydrocarbon-assimilating microorganisms
isolated from a temperate agricultural soil. Science Total Environmental 227:
237-47.
Chaerun,
S.K., Tazaki, K., Asada, R. & Kogure,
K. 2004. Interaction between clay minerals and hydrocarbon-utilizing
indigenous microorganisms in high concentrations of heavy oil: implications for
bioremediation. Environmental International 30: 911-922.
Chang, B.V., Wei, S.H. & Yuan, S.Y. 2001. Biodegradation of phenanthrene in soil. Journal Environmental Sciences Health 36: 177-87.
Das, K. & Mukherjee, A.K. 2007. Crude petroleum-oil biodegradation efficiency of Bacillus subtilisand Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East
India. Bioresources Technology 98:
1339-45.
Ghazali,
M.F., Rahman, R.N.Z.A., Salleh,
A.B. & Basri, M. 2004. Biodegradation of hydrocarbons in soil by microbial consortium. International Biodeterioration and Biodegradation 54:
61-67.
Hawle-Ambrosch,
E., Riepe, W., Dornmayr-Pfaffenhuemer,
M., Radax, C., Holzinger,
A. & Stan-Lotter, H. 2007. Biodegradation of fuel oil hydrocarbons by a mixed bacterial
consortium in sandy and loamy soils. Biotechnology Journal 2:
1564-1568.
Holt, J.G., Krieg, N.R., Sneath,
P.H.A., Stanley, J.T. & William, S.T. 1994. Bergey’s Manual of Determinative Bacteriology. Baltimore:
William and Wilkins.
Juang,
R.S. & Tsai, S.Y. 2006. Enhanced
biodegradation of mixed phenol and sodium salicylate by Pseudomonas putidain membrane contactors. Water Resources 40: 3517-26.
Mishra,
S., Jyot, J., Kuhad, R.C.
& Lal, B. 2001. In situ bioremediation potential of an oily sludge-degrading
bacterial consortium. Current Microbiology 43: 328-335.
Munoz, R., Diaz, L.F., Bordel, S.
& Villaverde, S. 2007. Inhibitory effects of catechol accumulation on
benzene biodegradation in Pseudomonas putidaF1
cultures. Chemosphere 68: 244-52.
Ohta,
Y., Maeda, M. & Kudo, T. 2001. Pseudomonas putidaCE2010 can degrade biphenyl by a mosaic
pathway encoded by the tod operon and cmtE, which are
identical to those of P. putidaF1 except for
a single base difference in the operator–promoter region of the cmt operon. Microbiology 147: 31-41.
Sathishkumar,
M., Binupriya, A.R., Baik,
S-H. & Yun, S-E. 2008. Biodegradation
of crude oil by individual bacterial strains and a mixed bacterial consortium
isolated from hydrocarbon contaminated areas. Clean 36(1): 92 – 96.
Szoboszlay,
S., Atzel, B. & Kriszt,
B. 2003. Comparative biodegradation examination of Pseudomonas aeruginosa(ATCC 27853) and other oil degraders on hydrocarbon
contaminated soil. Communication Agricultural Applied Biological Sciences 68:
207-10.
Toledo F.L., Calvo C., Rodelas, B. & Lopez, J.G. 2006. Selection and identification of bacteria isolated from waste crude oil with
polycyclic aromatic hydrocarbons removal capacities. Systematic and Applied
Microbiology 29: 244-252.
Vidali, M. 2001. Bioremediation: An
overview. Pure Applied Chemistry 73: 1163-1172.
Yu, H., Kim, B.J. & Rittmann, B.E.
2001. The roles of intermediates in biodegradation
of benzene, toluene, and p-xylene by Pseudomonas putidaF1. Biodegradation 12: 455-63.
Yuan, S.Y., Shiung, L.C. & Chang,
B.V. 2002. Biodegradation of polycylic aromatic hydrocarbons by inoculated microorganisms in soil. Bulletin
of Environmental Contamination and Toxicology 69: 66-73.
Yuan, S.Y., Wei, S.H. & Chang, B.V. 2000. Biodegradation of polycyclic aromatic hydrocarbons by a mixed
culture. Chemosphere 41: 1463-8.
Zajic,
E. & Supplison, B. 1972. Emulsification and degaradation of “Bunker C” fuel
oil by microorganisms. Biotechnology and Bioengineering 14: 331-334.
*Pengarang untuk surat-menyurat; email: antara@ukm.my
|