Sains Malaysiana 39(4)(2010): 557–563

Penentuan Aktiviti Antipengoksidaan Herba Tradisi Sarawak

Kacangma Leonurus sibiricus

(Determination of Antioxidation Activities of Sarawak’s Traditional  Herb Kacangma Leonurus sibiricus)

Chua Hun Pin*

Pusat Penyelidikan Teknologi Makanan

Institut Penyelidikan dan Kemajuan Pertanian Malaysia

Stesen MARDI Kuching, Lot 411, Blok 14, Petra Jaya, 93055 Kuching, Sarawak, Malaysia

Aminah Abdullah

Program Sains Makanan, Faculty Sains dan Teknologi

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia

Diserahkan: 14 Ogos 2009 / Diterima: 17 November 2009

ABSTRAK

Aktiviti pengoksidaan ekstrak etanol dan air daripada kacangma (Leonurus sibiricus) telah dikaji menggunakan tiga sistem ujian. Berdasarkan turutan aktiviti antipengoksida, ekstrak etanol kacangma kering didapati tinggi dalam sistem pengoksidaan asid linoleik (LP) 71.6%, sederhana dalam sistem penindasan superoksida xantin oksidase (XOD) 69.7% dan rendah dalam sistem penindasan radikal bebas 1,2-difenil-2-pikrilhidrazil (DPPH) 48.8%. Sebaliknya, ekstrak air kacangma kering didapati tinggi dalam ketiga-tiga sistem iaitu masing-masing 72.7, 76.3 dan 78.2%. Pengekstrakan air didapati lebih berkesan dalam mengeluarkan bahan antipengoksida daripada kacangma kering berbanding pengekstrakan alkohol.

Kata kunci: Aktiviti antipengoksidaan; Leonurus sibiricus; pengekstrakan; penindasan radikal; superoksida xantin oksidase

ABSTRACT

Antioxidant activities of ethanolic and aqueous extract from kacangma (Leonurus sibiricus) were determined by using three model systems. Based on the antioxidant activity range, ethanolic extract of dried kacangma was high in oxidation of linoleic acid lipid system (LP) 71.6%, moderate in xanthine oxidase superoxide scavenging activity (XOD) 69.7% and low in 1,2-diphenyl-2-picrylhydrazyl radical scavenging activity (DPPH) 48.8%. On the other hand, aqueous extract of dried kacangma was high in antioxidant activities for all three bioassays with mean percentages of 72.7, 76.3 and 78.2%, respectively. Extraction in water was more efficient than ethanol in extracting the antioxidants present in dried kacangma.

Keywords: Antioxidation activity; extraction; Leonurus sibiricus; radical scavenging; xanthine oxidase superoxide

RUJUKAN

Amarowicz, R., Pegg, R.B., Rahimi-Monghaddam, P., Barl, B. & Weil, J.A. 2004. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chemistry 84(4): 551-562.

Anon. 2008. Akta Makanan dan Peraturan-Peraturan. Kuala Lumpur: MDC Publishers Sdn Bhd.

Aruoma, O.I. 1998. Free radicals, oxidative stress and antioxidants in human health and disease. Journal of American Oil Chemists’ Society 75(2): 199-212.

Blois, M.S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26: 1199-1200.

Brand-Williams, W., Cuvelier, M.E. & Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft+Technologie 28(1): 25-30. 

Chang, W.S., Lin C.C., Chuang, S.C. & Chiang, H.C. 1996. Superoxide anion scavenging effect of coumarins. American Journal of Chinese Medicine 24(1): 11-17.

Chen, H.M., Muramoto, K., Yamauchi, F. & Nokihara, K. 1996. Antioxidant activity of design peptides based on antioxidative peptide isolated from digest of a soybean protein. Journal of Agricultural and Food Chemistry 44(9): 2619-2623.

Dorman, H.J.D., Peltoketo, A., Hiltunen, R. & Tikkanen, M.J. 2003. Characterization of the antioxidant properties of de-odourized aqueous from selected Lamiaceae herbs. Food Chemistry 83(2): 255-262.

Duh, P.D. & Yen, G.C. 1997. Antioxidative activity of three herbal water extracts. Food Chemistry 60(4): 639-645.

Frankel, E.N., Huang, S.W., Aeschbach, R. & Prior, E. 1996. Antioxidant activity of a rosemary extract and its constituents, carnosic acid, carnosol, and rosmarinic acid, in bulk oil and oil-in-water emulsion. Journal of Agricultural and Food Chemistry 44(1): 131-135. 

Giese, J. 1999. Tests for nutraceutical products. Food Technology 53(10): 93.

Halliwell, B. & Gutteridge, J.M.C. 2007. Free Radicals in Biology and Medicine. Ed. ke-4. Oxford: Clarendon Press.

Huang, S.S., Liao J.P. & Tang, Y.J. 2005. Advances in the studies of glandular hairs and secretion in Labiatae plants. Journal of Tropical and Subtropical Botany 13(5): 452-456.

Kaur, C. & Kapoor, H.C. 2002. Antioxidant activity and total phenolic content of some Asian vegetables. International Journal of Food Science and Technology 37: 153-161.

Lampi, A.M., Piironen, V., Hopia, A. & Koivistoinen, P. 1997. Characterization of the oxidation of rapeseed and butter oil triacylglycerols by four analytical methods. Lebensmittel-Wissenschaft und-Technologie 30(8): 807-813.

Loliger, J. 1991. The use of antioxidants in foods. Dlm. Free Radical and Food Additives, edited by Aruoma, O.I. & Halliwell, B. London: Taylor & Francis.

Masteikova, R., Muselik, J., Bernatoniene, J., Majiene, D., Savickas, A., Malinauskas, F., Bernatoniene, R., Peciura, R., Chalupova, Z. & Dvorackova, K. 2008. Antioxidant activity of tinctures prepared from hawthorn fruits and motherwort herb. Ceska a Slovenska Farmacie 57(1): 35-38.

Matkowski, A. & Piotrowska, M. 2006. Antioxidant and free radical scavenging activities of some medicinal plants from the Lamiaceae. Fitoterapia 77(5): 346-353.

Mau, J.L., Lin, H.C. & Song, S.F. 2002. Antioxidant properties of several specialty mushrooms. Food Research International 35(6): 519-526.

Murakami, M., Yamaguchi, T., Takamura, H. & Matoba, T. 2006. Effects of Ascorbic Acid and α-Tocopherol on Antioxidant Activity of Polyphenolic Compounds. Journal of Food Science 68(5): 1622-1625.

Nam, S.H. & Kang, M.Y. 2004. Antioxidant activity of 13 medicinal plants. Pharmaceutical Biology 42(6): 409-415.

Osawa, T. & Namiki, N. 1981. A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves. Agricultural and Biological Chemistry 45(3): 735-739.

Qu, G.Z., Si, C.L. & Wang, M.H. 2006. Antioxidant constituents from Leonurus japonicus. Natural Product Sciences 12(4): 197-200.

Rajalakshmi, D. & Narasimhan, S. 1995. Food antioxidants: Source and methods of evaluation. Dlm. Food Antioxidants: Technological, Toxicological and Health Perspective, Modhavi, D.L., Deshpande, S.S. & Salunkhe, D.K. (eds.) New York: Marcel Dekker Inc.

SAS. 1994. SAS User’s Guide: Statistics Version 5. Cary, NC.: SAS Institute Inc.

Schwarz, K., Bertelsen, G., Nissen, L.R., Gardener, P.T., Heinonen, M.I., Hopia, A., Huyah-Ba, T., Lambelet, P., McPhail, D., Skibsted, L.H. & Tijburg, L. 2001. Investigation of plant extracts for the protection of processed foods against lipid oxidation. Comparison of antioxidant assays based on radical scavenging, lipid oxidation and analysis of the principal antioxidant compounds. European Food Research Technology 212(3): 319-328.

Tanaka, H., Yamaba, H., Okada, T., Katada, T. & Nakata, S. 2001. Stimulating effects of Yakumosou (Leonurus sibiricus L.) on intracellular SOD activity in skin. Journal of Japanese Cosmetic Science Society 25(2): 74-80.

Vimala, S., Mohd Ilham, A., Abdull Rashih, A. & Rohana, S. 2003. Nature’s Choice To Wellness: Antioxidant Vegetable/Ulam. Siri Alam dan Rimba No.7. Kepong: Forest Research Institute Malaysia.

*Pengarang untuk surat-menyurat: hpchua@mardi.gov.my