Sains Malaysiana 39(6)(2010): 909–912

Development and Characterization of Elaeis oleifera Microsatellite Markers

(Pembangunan dan Pencirian Penanda Mikrosatelit Elaeis oleifera)

Noorhariza Mohd Zaki*¹, Ismanizan Ismail^2,3, Rozana Rosli²

Ting Ngoot Chin¹ & Rajinder Singh¹

¹Malaysian Palm Oil Board

6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia

²Pusat Pengajian Biosains dan Bioteknologi

Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia

43600, Bangi, Selangor, Malaysia

³Institut Biologi Sistem (INBIOSIS)

Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia

Diserahkan: 7 Oktober 2009 / Diterima: 11 Januari 2010

ABSTRACT

Ten Elaeis oleifera microsatellite markers were developed and characterised from 1500 sequences of the E. oleifera genomic library. The markers were utilised to assess the genetic diversity of E. oleifera germplasm collections from four South American countries (Colombia, Costa Rica, Panama and Honduras). The number of alleles per-locus varied from 2 to 11 and the observed and expected heterozygosity ranged from 0.0685 to 0.9853 and 0.1393 to 0.8216 respectively. Majority of the markers showed transferability to Elaeis guineensis while two markers showed transferability across Arecaceae taxa. These E. oleifera microsatellite markers are expected to become useful tools to determine the population structure and conservation of E. oleifera populations.

Keywords: E. oleifera; genomic; microsatellite; transferability

ABSTRAK

Sebanyak sepuluh penanda mikrosatelit genomik Elaeis oleifera telah dibangunkan dan dicirikan daripada sejumlah 1500 klon di dalam perpustakaan genomik E. oleifera. Keupayaan penanda yang dibangunkan ini digunakan bagi menganalisis kepelbagaian genetik di dalam koleksi germplasma dari empat negara di Amerika Selatan (Colombia, Costa Rica, Panama dan Honduras). Bilangan alel per-lokus yang dicerap adalah di dalam lingkungan 2 hingga 11 manakala heterozigositi yang dicerap dan dijangka adalah masing-masing 0.0685 hingga 0.9853 dan 0.1393 hingga 0.8216. Majoriti penanda mikrosatelit menunjukkan kebolehpindahan kepada Elaeis guineensis manakala dua penanda berjaya menunjukkan kebolehpindahan merentasi takson famili Arecaceae. Penanda-penanda mikrosatelit yang berjaya dibangunkan ini di jangka dapat menjadi penanda dalam menentukan struktur populasi dan pemuliharaan populasi E. oleifera.

Kata kunci: E. oleifera; genomik; kebolehpindahan; mikrosatelit

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*Pengarang untuk surat-menyurat; email: hariza@mpob.gov.my