Sains Malaysiana 40(10)(2011): 1115–1122
Screening of Lactobacillus Strains Against Salmonella Both Isolated from Malaysian Free-Range Chicken Intestine for Use as
Probiotic
(Penyaringan Strain Lactobacillus Melawan Salmonella yang Kedua-duanya Dipencilkan daripada
Usus Ayam Kampung
Malaysia untuk Kegunaan Sebagai Probiotik)
Andri Hutari, Waleed Shaker Jaseem, AidiL Abdul Hamid & Wan Mohtar Wan Yusoff*
Department of Microbiology, School
of Biosciences and Biotechnology
Faculty of
Science and Technology, Universiti Kebangsaan Malaysia
43600 Bangi,
Selangor D.E. Malaysia
Diserahkan: 15 Julai 2010/Diterima:
10 November 2010
ABSTRACT
A total of eight strains of Lactobacillus and two strains of Salmonella were isolated from free-range Malaysian
chickens intestine. Evaluation based on in vitro studies included
aggregation, co-aggregation, growth with bile salts, tolerance to acidic pH,
and inhibitory activity were carried out. The isolated Lactobacillus were Lactobacillus fermentum IA, Lactobacillus
fermentum IB, Lactobacillus fermentum IC, Lactobacillus fermentum ID, Lactobacillus
salivarius subsp. salicinus IE, Lactobacillus
salivarius subsp. salicinus IF, Lactobacillus
salivarius subsp. salivarius IG, and Lactobacillus spp. IH.
The corresponding isolated Salmonella were Salmonella spp. 3B21
and Salmonella spp. 1A12. The ability of aggregation and also tolerance
to pH 2.5 are found in Lactobacillus fermentum ID, Lactobacillus salivarius subsp. salicinus IF, Lactobacillus salivarius subsp. salivarius IG, and Lactobacillus spp. IH.
The isolate most resistance to 1% bile salts is Lactobacillus fermentum ID but
observed to be weak in inhibitory activity against Salmonella spp. The
best co-aggregation and strongest inhibitory activity against Salmonella spp.
was observed in Lactobacillus salivarius subsp. salivarius IG.
Despite being not so resistant in the presence of bile salts 0.5 and 1% (w/v),
the lag time in the presence of bile salts 0.3% (w/v) of Lactobacillus
salivarius subsp. salivarius IG and also for Lactobacillus spp. IH are the shortest. Based on good aggregation
properties, the best co-aggregation, tolerance to acidic pH 2.5 and bile salts
0.3% (w/v) and strongest inhibitory activity against Salmonella spp., Lactobacillus
salivarius subsp. salivarius IG comes out as the best
candidate as probiotic for chicken.
Keywords: Inhibitory
activity; Lactobacillus; Malaysian free-range chicken; Salmonella
ABSTRAK
Sebanyak lapan strain Lactobacillus dan dua strain Salmonella dipencilkan daripada usus ayam kampung
Malaysia. Penilaian berdasarkan kajian in vitro seperti ujian agregasi,
koagregasi, kerintangan terhadap garam hempedu, kerintangan terhadap pH asid,
dan ujian aktiviti perencatan telah dilakukan. Pencilan Lactobacillus tersebut
ialah Lactobacillus fermentum IA, Lactobacillus
fermentum IB, Lactobacillus fermentum IC, Lactobacillus fermentum ID, Lactobacillus
salivarius subsp. salicinus IE, Lactobacillus
salivarius subsp. salicinus IF, Lactobacillus
salivarius subsp. salivarius IG, dan Lactobacillus spp. IH.
Sedangkan pencilan salmonella yang didapatkan ialah Salmonella spp. 3B21
and Salmonella spp. 1A12. Kemampuan agregasi dan juga ketahanan terhadap
pH 2.5 dijumpai pada Lactobacillus fermentum ID, Lactobacillus salivarius subsp. salicinus IF, Lactobacillus salivarius subsp. salivarius IG,
dan Lactobacillus spp. IH. Pencilan yang paling tahan
terhadap garam hempedu 1% ialah Lactobacillus fermentum ID,
tetapi Lactobacillus tersebut menunjukkan aktiviti perencatan yang lemah
terhadap Salmonella spp. Koagregasi terbaik dan aktiviti perencatan yang
paling kuat terhadap Salmonella spp. dijumpai pada Lactobacillus
salivarius subsp. salivarius IG. Meskipun tidak begitu tahan
di dalam kehadiran garam hempedu 0.5 dan 1% (w/v), masa lag Lactobacillus
salivarius subsp. salivarius IG dan juga Lactobacillus spp. IH di dalam kehadiran garam hempedu 0.3% (w/v) adalah
yang paling singkat. Berdasarkan ciri-ciri agregasi yang baik, koagregasi yang
terbaik, kerintangan terhadap pH 2.5 dan garam hempedu 0.3% (w/v), serta
aktiviti perencatan yang paling kuat terhadap Salmonella spp., Lactobacillus
salivarius subsp. salivarius IG keluar sebagai calon
terbaik probiotik ayam.
Kata kunci: Aktiviti perencatan; ayam kampung Malaysia; Lactobacillus; Salmonella
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*Pengarang untuk surat-menyurat; email:
wantar@ukm.my
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