Sains Malaysiana 40(10)(2011): 1123–1127
Blastocystis hominis: Kehadirannya di dalam Sampel Feses Kanak-kanak
Orang Asli di Pos Lenjang, Pahang, Malaysia
(Blastocystis
hominis: Its Presence in the Faecal Samples of
Orang Asli Children at Pos Lenjang, Pahang, Malaysia)
Mohamed
Kamel Abd. Ghani
Jabatan Sains
Bioperubatan, Fakulti Sains Kesihatan Bersekutu, Universiti Kebangsaan Malaysia
50300 Jalan Raja
Muda Abdul Aziz, Kuala Lumpur, Malaysia
Hartini Yusof
Fakulti Sains
Kesihatan, Universiti Teknologi MARA, Kampus Puncak Alam
42300 Bandar
Puncak Alam, Selangor, Malaysia
Diserahkan:
15 Julai 2010 / Diterima: 4 Januari 2011
ABSTRAK
Blastocystis
hominis merupakan antara protozoa yang paling biasa ditemui di dalam sampel
feses manusia di seluruh dunia. Prevalens
infeksi protozoa ini adalah lebih tinggi di kalangan mereka yang tinggal di
negara membangun berbanding negara maju. Seramai 71
orang kanak-kanak Orang Asli dari Pos Lenjang, Pahang telah menjadi subjek
dalam kajian ini. Bagi kajian yang lebih terperinci, kumpulan
kanak-kanak ini telah dibahagikan menurut jantina dan umur. Sampel feses
dikumpul dan setiap sampel diperiksa dengan menggunakan 3 teknik diagnostik
iaitu teknik apusan langsung, konsentrasi formalin-eter dan perwarnaan trikrom
bagi tujuan pengesanan dan pengenalpastian Blastocystis hominis.
Prevalens infeksi Blastocystis hominis di kalangan kanak-kanak Orang
Asli adalah sangat tinggi iaitu 93%. Kanak-kanak perempuan
didapati lebih ramai terinfeksi (97.5%) berbanding kanak-kanak lelaki (87.1%)
walaupun secara statistiknya tidak signifikan (p>0.05). Protozoa ini juga telah menginfeksi kesemua kanak-kanak prasekolah
(100%) manakala kanak-kanak yang bersekolah turut menunjukkan prevalens infeksi
yang tinggi iaitu 86.5%. Daripada segi diagnosis, teknik perwarnaan
trikrom didapati paling sensitif dan ia dapat
mengenalpasti kesemua (66) sampel feses yang positif dengan Blastocystis
hominis. Ini diikuti dengan teknik konsentrasi
formalin-eter (43 sampel) dan teknik apusan langsung (18 sampel) (p<0.05). Prevalens infeksi Blastocystis hominis yang tinggi di kalangan
kanak-kanak Orang Asli adalah berhubungkait dengan pelbagai faktor termasuk
status sosioekonomi yang rendah, budaya, kekurangan kemudahan asas dan tahap
pengetahuan mengenai penjagaan kesihatan serta kebersihan diri yang rendah.
Selain itu, peningkatan prevalens infeksi dalam kajian ini menunjukkan
pentingnya penggunaan teknik diagnostik yang lebih berkesan di dalam
pemeriksaan rutin bagi memperolehi hasil diagnosis yang lebih tepat.
Kata kunci: Blastocystis
hominis; Malaysia; Orang Asli
ABSTRACT
Blastocystis hominis is
among the protozoa that is commonly found in the human
faecal samples throughout the world. The prevalence of infection is higher
among people living in developing countries than developed countries.
Seventy-one Orang Asli children at Pos Lenjang, Pahang were enrolled in this
study. For more detailed study, the children were divided according to gender
and age groups. Faecal samples were collected and examined for the presence of Blastocystis
hominis using three different diagnostic techniques, namely direct smear,
formalin-ether concentration and trichrome stain. The prevalence of Blastocystis
hominis infection among Orang Asli children was very high (93%). The
infection was higher in females (97.5%) compared to males (87.1%) eventhough it
was not statistically significant (p>0.05). All the preschoolers (100%) were
found to be infected with the protozoa, while the school going children also
showed a high prevalence of infection (86.5%). From the diagnosis aspect,
trichrome stain was the most sensitive technique, detecting all
the fecal samples (66) positive for Blastocystis hominis (p<0.05).
It was followed by formalin-ether concentration (43 samples) and direct smear
(18 samples). A high prevalence of Blastocystis hominis infection among
Orang Asli children was closely related with various factors including low
socioeconomic status, culture, lack of basic facilities and poor knowledge in
health and personal hygiene. On the other hand, the result of the present study
indicated that it is important to employ effective diagnostic technique in
routine parasitological examination for more accurate diagnosis.
Keywords: Blastocystis hominis; Malaysia; Orang Asli
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*Pengarang
untuk surat-menyurat; email: mkamal@medic.ukm.my
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