Sains Malaysiana
40(2)(2011): 125–133
Molecular
Phylogeny of Holothuria (Mertensiothuria) leucospilota (Brandt
1835) as Inferred from Cytochrome C Oxidase I Mitochondrial DNA Gene Sequences
Filogeni Molekul Holothuria
(Mertensiothuria) leucospilota (Brandt 1835) Berdasarkan Penjujukan Gen
Sitokrom C Oksidase I Mitokondria DNA
Kamarul Rahim Kamarudin1,*,
‘Aisyah Mohamed Rehan2, Ridzwan Hashim2, Gires Usup3,
Hajar Fauzanahmad2, Mohd Hanafi Anua2 & Mohd Yamanidris2
1School of Biological Sciences (SBS), The University of Auckland
Private Bag 92019, Auckland 1142, New Zealand
2Sea Cucumber (Echinodermata: Holothuroidea)
Research Group Institute
of Oceanography & Maritime Studies (INOCEM)
Kulliyyah
of Science, International Islamic University Malaysia (IIUM)
Jalan
Istana, Bandar Indera Mahkota, 25200 Kuantan, Pahang Darul Makmur, Malaysia
3Marine Science Programme
School
of Environmental & Natural Resource Sciences, Faculty of Science &
Technology
Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia
Diserahkan: 5 November 2009 / Diterima: 16 Jun 2010
ABSTRACT
Holothuria
(Mertensiothuria) leucospilota (Brandt 1835), white threads fish or locally
known as bat puntil is currently considered as the most abundant sea cucumber
species in Malaysia. This study aimed to generate the genetic profile of H.
leucospilota from Malaysia and then to determine the phylogenetic
relationship between H. leucospilota and other members of genus Holothuria using partial sequences of cytochrome c oxidase I (COI)
mitochondrial DNA (mtDNA) gene. In this study, specimens of H.
leucospilota were collected from Intan Besar Island, Langkawi, Kedah Darul
Aman in the west coast of Peninsular Malaysia. Three main methods namely
neighbour joining, maximum parsimony and maximum likelihood were used for the
phylogenetic tree reconstruction. Tree topologies showed that H. leucospilota
has its own monophyletic clade clearly distinct from the other species. The
pairwise genetic distance calculated further supported these findings. In
addition, the results also should that the COI mtDNA
gene is capable to unravel the phylogenetic relationship of H. leucospilota.
Keywords: Holothuria
leucospilota; molecular systematics; partial sequences of cytochrome oxidase
I gene; phylogeny tree
ABSTRAK
Holothuria
(Mertensiothuria) leucospilota (Brandt 1835) atau nama tempatannya bat
puntil kini dianggap sebagai spesies timun laut yang paling banyak
taburannya di Malaysia. Kajian ini bertujuan untuk mendapatkan profil genetik H.
leucospilota dan seterusnya menentukan perhubungan filogenetik antara H.
leucospilota dari Malaysia dan ahli-ahli lain daripada genus Holothuria
menggunakan jujukan separa gen sitokrom c oksidase I (COI)
mitokondria DNA (mtDNA). Dalam kajian ini, spesimen H.
leucospilota diperolehi dari Intan Besar Island, Langkawi, Kedah Darul Aman
di pantai barat Semenanjung Malaysia. Tiga kaedah iaitu kaedah hubungkait
jiran, parsimoni maksimum dan kaedah hubungkait maksimum telah digunakan untuk
pembinaan pohon-pohon filogenetik. Topologi pohon menunjukkan H. leucospilota mempunyai
klusternya sendiri yang monofiletik yang jelas berbeza daripada spesies-spesies
yang lain. Pengiraan jarak genetik secara berpasangan seterusnya telah
menyokong keputusan-keputusan tersebut. Di samping itu, keputusan kini juga
menunjukkan bahawa gen COI mtDNA mempunyai kapasiti untuk
merungkaikan perhubungan filogenetik H. leucospilota.
Kata kunci:
Sistematik molekul; Holothuria leucospilota; jujukan separa gen sitokrom c
oksidase I mitokondria DNA, pohon filogen
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*Pengarang untuk
surat-menyurat; email: physique481@yahoo.co.uk
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