Sains Malaysiana 40(4)(2011): 317–321
Anticholesterol Activity of Velvet Bean (Mucuna
pruriens L.) towards Hypercholesterolemic Rats
(Aktiviti
Antikolestrol daripada Kacang Velvet (Mucuna pruriens L.) terhadap Tikus
Hiperkolestromik)
Hana Ratnawati*
& Wahyu Widowati
Medical Research Center, Faculty of Medicine, Maranatha
Christian University
Jl. Surya Sumantri 65, Bandung – 40164, Indonesia
Diserahkan: 9 Disember 2009 / Diterima 17 Julai 2010
ABSTRACT
Hypercholesterolemia is
the main risk factor of atherosclerosis which is directly related to coronary
heart disease. Velvet bean (Mucuna pruriens L.) is
predicted to have potential anticholesterolemia since a previous study showed
that this plant had high antioxidant activity. The purpose of this research was
to determine the anticholesterol activity of crude extract and ethyl acetate
fraction of velvet bean compared to simvastatin and vitamin E in decreasing the
plasma level of total cholesterol, LDL and triglyceride and
increasing the HDL level on hypercholesterolemic
rats. Thirty male Wistar rats were divided into ten groups. Group I was fed
with standard diet as negative control group and all the other groups were fed
with high-fat diet and were given fructose solution. High-fat diet and fructose
solution increased the level of total cholesterol, LDL-cholesterol
and triglyceride and decreased the level of HDL-cholesterol
significantly compared to the negative control group. The treatment groups were
given 3 various doses of crude extract (50, 100, 200 mg/kgBW) and ethyl acetate
fraction (15, 30, 60 mg/kgBW) of velvet bean, simvastatin 2.7 mg/kgBW and
vitamin E 60 mg/kgBW daily for ten days. The data were analyzed by one way
Analysis of Variance (ANOVA) and Duncan’s Post Hoc Test. The
best results were shown by 15 mg/kgBW of ethyl acetate fraction in decreasing
total cholesterol and 60 mg/kgBW of ethyl-acetate fraction for the LDL-cholesterol,
200 mg/kgBW of crude extract in increasing HDL-cholesterol
and 50 and 200 mg/kgBW of crude extract and 60 mg of ethyl acetate fraction in
lowering the triglyceride.
Keywords: Anticholesterol;
hypercholesterolemia; Mucuna pruriens L.; velvet bean
ABSTRAK
Hiperkolesterolemia adalah
factor utama aterosklerosis yang berkaitan dengan penyakit jantung koronari.
Kacang Velvet (Mucuna pruriens L.) diramalkan mempunyai potensi
antikolestrolemia kerana kajian sebelumnya menunjukkan bahawa tanaman ini
mempunyai aktiviti antioksida yang tinggi. Tujuan kajian ini adalah untuk
menentukan aktiviti antikolestrol ekstrak kasar dan bahagian etil asetat
daripada kacang velvet dibandingkan dengan simvastatin dan vitamin E dalam
menurunkan jumlah tahap kolesterol dalam plasma, LDL dan
trigliserida serta meningkatkan kadar HDL pada tikus
hiperkolesterolemia. Tiga puluh tikus jantan Wistar dibahagikan kepada sepuluh
kumpulan. Kumpulan I diberikan diet standard sebagai kumpulan kawalan negatif
dan semua kumpulan yang lain diberikan diet tinggi lemak dan larutan fruktosa.
Diet tinggi lemak dan larutan fruktosa dapat meningkatkan tahap jumlah
kolesterol, kolestrol LDL dan trigliserida serta menurunkan
kadar kolestrol HDL secara signifikan jika
dibandingkan dengan kumpulan kawalan negatif. Kumpulan perlakuan diberikan 3
dos pelbagai ekstrak kasar (50, 100, 200 mg/kgBW) dan bahagian etil asetat (15,
30, 60 mg/kgBW) daripada kacang velvet, simvastatin 2.7 mg/kgBW dan vitamin E
60 mg/kgBW setiap hari selama sepuluh hari. Data dianalisis dengan Analisis
Varian satu hala (ANOVA) dan Kajian Duncan Post Hoc.
Keputusan yang terbaik ditunjukkan oleh bahagian etil asetat dengan 15 mg/kgBW
menurunkan jumlah kolestrol dan 60 mg/kgBW bahagian etil asetat kolestrol LDL,
200 mg/kgBW ekstrak kasar dalam meningkatkan kolestrol HDL dan
50 dan 200 mg/kgBW ekstrak kasar dan 60 mg bahagian etil asetat dapat menurunkan
trigliserida.
Kata kunci: Antikolesterol; hiperkolesteralemia; Mucuna
pruriens L.; kacang velvet
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*Pengarang
untuk surat-menyurat; email: hanna_ratnawati@yahoo.com
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