Sains Malaysiana 40(4)(2011): 369–372
Effects of Pink Guava (Psidium
guajava) Puree Supplementation on Antioxidant Enzyme Activities and Organ
Functions of Spontaneous Hypertensive Rat
(Kesan
Suplementasi Puri Jambu Batu Merah (Psidium guajava) ke Atas Aktiviti
Enzim
Antioksidan dan Fungsi Organ Tikus Hipertensi Spontan)
Norazmir Md Nor*
Department
of Nutrition & Dietetics, Faculty of Health Sciences
Universiti
Teknologi MARA, Puncak Alam Campus 42300 Puncak Alam, Selangor D.E., Malaysia
Ayub
Mohd Yatim
Deceased
Diserahkan:
7 Januari 2010 / Diterima: 21 Jun 2010
ABSTRACT
This study was aimed to determine the effects of pink guava (Psidium guajava) puree
supplementation on enzyme activities, kidney and liver function tests of
Spontaneous Hypertensive Rats (SHR). Twenty-four male SHR were
divided into four groups (control, CG (distilled water); low
dosage group, LDG (0.5 g/kg body weight); medium dosage group, MDG (1.0
g/kg body weight); high dosage group, HDG (2.0g/kg body weight)). The
rats were given pink guava puree via force-feeding and fed rat pellets ad
libitum for 28 days in individual cages at 25±2°C. At the end of experiment,
the rats were fast overnight (12 to14 h) and euthanized under an anesthetic
condition with ether, and blood was collected from the portal vein or posterior
vena cava. The specific activities of glutathione peroxidase (GPx) was
significantly higher in LDG (2332.5±81.8 U/L), MDG (2424.8±97.1
U/L) and HDG (2594.6±82.8 U/L) respectively, as compared to CG (2171.8±65.9
U/l). Significant differences were also seen in glutathione reductase (GR)
activities among all treated groups (LDG (132.5±11.8 U/L), MDG (141.5±16.4
U/L), HDG (148.8±13.2 U/L) compared to CG (126.1±14.2
U/L)). Liver function tests for total antioxidant status (TAS),
alanine aminotransferase (ALT), aspartate aminotransferase (AST),
lactate dehydrogenase (LDH) and γ-glutamyl
transpeptidase (GGT) showed significant differences in
the treated group compared to control group. In conclusion, this study shows
pink guava puree supplementation increase antioxidant enzyme activity in SHR’s
blood concentration.
Keywords: Antioxidant; enzyme activity; organ functions; pink
guava; spontaneous hypertensive rats
ABSTRAK
Kajian dijalankan untuk menentukan kesan pemberian suplemen puri
jambu batu merah (Psidium
guajava) ke atas aktiviti enzim, dan ujian kefungsian ginjal dan hati tikus
teraruh hipertensi (SHR). Sebanyak 24 ekor SHR dibahagi
kepada 4 kumpulan (kawalan, CG (air suling); dos rendah, LDG (0.5
g/kg berat badan); dos sederhana, MDG (1.0 g/kg berat badan); dos
tinggi, HDG (2.0 g/kg berat badan)). Tikus diberi puri jambu batu
merah melalui suap-paksa dan pelet tikus secara ad libitum selama 28 hari dan
diletakkan dalam sangkar individu pada suhu 25±2°C. Akhir kajian, tikus dipuasa
semalaman (12-14 jam) dan dibiuskan di bawah pengaruh eter, dan darah diambil
dari vena kava posterior. Aktiviti spesifik bagi glutation peroksida (GPx)
adalah tinggi secara signifikan dalam LDG (2332.5±81.8 U/L), MDG (2424.8±97.1
U/L) dan HDG (2594.6±82.8 U/L) masing-masing, berbanding CG (2171.8±65.9
U/L). Terdapat perbezaan signifikan pada aktiviti glutation reduktase (GR)
dalam kumpulan rawatan (LDG (132.5±11.8 U/L), MDG (141.5±16.4
U/L), HDG (148.8±13.2 U/L) berbanding CG (126.1±14.2
U/L)). Ujian fungsi hati bagi status antioksidan total (TAS),
alanin aminotransferase (ALT), aspartat aminotransferase (AST),
laktat dehidrogenase (LDH) dan γ-glutamil
transpeptidase (GGT) menunjukkan perbezaan signifikan
dalam kumpulan rawatan berbanding kumpulan kawalan. Kajian menunjukkan
suplementasi puri jambu batu merah meningkatkan aktiviti enzim antioksidan
dalam darah tikus hipertensi spontan.
Kata kunci: Antioksidan; aktiviti enzim; fungsi organ; jambu
batu merah; tikus hipertensi spontan
RUJUKAN
Ayub, M.Y., Norazmir, M.N.,
Mamot, S., Jeeven, K. & Hadijah, H. 2010. Anti-hypertensive effect of pink
guava (Psidium guajava) puree on Spontaneous Hypertensive Rats. International
Food Research Journal 17: 89-96.
Cheng, J.T. & Yang, R.S.
1983. Hypoglycemic effect of guava juice in mice and human subjects. The
American Journal of Chinese Medicine 11: 74-76.
Datta, S., Sinha, S. &
Bhattacharyya, P. 1999. Effect of an herbal protein, CI-I, purified from Cajanus
indicus, in models of liver failure in mice. Drug Development Research 48:
76-83.
Hadijah, H., Ayub, M.Y.,
Zarida, H. & Normah, A. 2004. Hypoglycemic activity of Morinda
citrifolia extract in normal and streptozotocin-induced diabetic rats. Journal
of Tropics, Agriculture and Food Science 32(1): 39-44.
Heywood, R. 1983. Long-term
toxicity. In: Animals and Alternatives in Toxicity Testing (Balls, M.,
Riddell, R.J. and Worden, A.N., ed.) p. 79-89. London: Academic Press.
Jaiarj, P., Khoohaswan, P.,
Wongkrajang, Y., Peungvicha, P., Suriyawong, P. & Sumal Saraya, M.L. 1999.
Anticough and antimicrobial activities of Psidium guajava Linn, leaf
extract. Journal of Ethnopharmacology 67: 203-212.
Kadiri, S., Arije, A. &
Salako, B.L. 1999. Tradititional herbal preparations and acute renal failure in
South West Nigeria. Tropical Doc 29(4): 244-246.
Lamela, M., Cadavid, I. &
Calleja, J.M. 1986. Effects of Lythrum salicaria extracts on
hyperglycemic rats and mice. Journal of Ethnopharmacology 51: 153-160.
Lucia, R.D., Sertie, J.A.A.,
Camarco, E.A. & Panizza, S. 1997. Pharmacological and toxicological studies
on Centella asiatica extract. Fitoterapia 51: 413-416.
Lutterodt, G.D. 1989.
Inhibition of gastrointestinal release of acetylcholine by quercetin as a
possible mode of action of Psidium guajava leaf extracts in the
treatment of acute diarrhoeal disease. Journal of Ethnopharmacology 25:
235-247.
Marshall, W.J. 2000. Clinical
chemistry. 4th edition. Edinburgh: Mosby.
Matsuo, T., Hanamure, N.,
Shimoi, K., Nakamura, Y. & Tomita, I. 1994. Identification of
(+)-gallocatechin as a bio-antimutagenic compound in Psidium guava leaves. Phytochemistry 36: 1027-1029.
Moshi, M.J., Lutale, J.J.K.,
Rimoy, G.H., Abbas, Z.G., Josiah, R.M. & Andrew, B.M. 2001. The effect of Phyllanthus
amarus aqueous extract on blood glucose in non-insulin dependent diabetic
patients. Phytochemical Research 15: 577-580.
Obatomi, D.K., Bikomo, E.O.
& Temple, V.J. 1994. Anti-diabetic properties of the African mistletoe in
streptozotocin-induced diabetic rats. Journal of Ethnopharmacology 43:
13-17.
Olajide, O.A., Awe, S.O.
& Makinde, J.M. 1999. Pharmacological studies on the leaf of Psidium
guajava. Fitoterapia 70: 25-31.
Prince, P.S. & Menon,
V.P. 1999. Antioxidant activity of Tinospora cordifolia roots in
experimental diabetes. Journal of Ethnopharmacology 65: 277-281.
Thaipong, K., Unaroj, B.,
Kevin, C., Luis, C.Z. & David, H.B. 2006. Comparison of ABTS, DPPH, FRAP
and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal
of Food Composition and Analysis 19: 669-675.
Vaughn, G. 1999. Understanding
and Evaluating Common Laboratory Test. Stamford: Appleton & Lange.
WHO. 1992. Research
Guidelines for Evaluating the Safety and Efficacy of Herbal Medicines.
Geneva: World Health Organization.
*Pengarang untuk surat-menyurat;
email: azmir2790@puncakalam.uitm.edu.my
|