Sains Malaysiana 40(5)(2011): 457–466

Kesan Sitotoksik dan Aktiviti Anti-MrsaEkstrak Metanol Phyllanthus gracilipes dan Phyllanthus columnaris

 (Cytotoxic Effect and Anti-MRSA Activity of Methanolic Extracts of Phyllanthus gracilipes and Phyllanthus columnaris)

G.M. Fazari, A. Azilawaty & I. Nazlina*

Pusat Pengajian Biosains dan Bioteknologi, Fakulti Sains dan Teknologi

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E., Malaysia

W.A. Yaacob

Pusat Pengajian Sains Kimia dan Teknologi Makanan, Fakulti Sains dan Teknologi

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E., Malaysia

Diserahkan: 1 April 2010 / Diterima: 1 September 2010

ABSTRAK

Satu kajian telah dijalankan untuk menguji kesan sitotoksik dan aktiviti antibakteria ekstrak metanol dari bahagian berbeza tumbuhan Phyllanthus gracilipes dan P. columnaris. Pengekstrakan dilakukan pada suhu bilik dan menggunakan alat-radas Soxhlet untuk menghasilkan 22 ekstrak. Ujian fitokimia ke atas ekstrak Soxhlet menunjukkan kehadiran saponin dalam semua ekstrak manakala steroid terdapat dalam ekstrak semua bahagian P. gracilipes dan 3 daripada ekstrak P. columnaris. Ujian sitotoksik ke atas kultur sel Vero menunjukkan hanya empat ekstrak yang sitotoksik dengan nilai LC50 di bawah 20 μg/mL iaitu ekstrak suhu bilik kulit-batang P. gracilipes, ekstrak Soxhlet kayu-batang dan daun P. gracilipes serta ekstrak suhu bilik kayu-akar P. columnaris. Ekstrak suhu bilik dan Soxhlet kayu-batang P. gracilipes serta semua ekstrak suhu bilik P. columnaris merencat pertumbuhan 13 pencilan Staphylococcus aureus Rintang-Metisillin (MRSA) yang diuji.

Kata kunci: Aktiviti antibakteria; kesitotoksikan; Phyllanthus columnaris; Phyllanthus gracilipes; sel Vero

ABSTRACT

A study was carried out to test the cytotoxic effect and antibacterial activity of methanol extracts from different parts of Phyllanthus gracilipes and P. columnaris. The extraction was done at room temperature and using a Soxhlet apparatus to produce 22 extracts. Phytochemical test on the extracts produced using Soxhlet showed the presence of saponins in all the extracts whilst steroids were found in all P. gracilipes parts and only in 3 extracts of P. columnaris. Cytotoxic tests on Vero cell culture revealed four extracts were cytotoxic with LC50 values below 20 μg/mL i.e. room temperature extract of P. gracilipes stem-bark, Soxhlet extracts of P. gracilipes stem-wood and leaves, and room temperature extract of P. columnaris root-wood. Room temperature and Soxhlet extracts of P. gracilipes stem-wood, and all room temperature extracts of P. columnaris inhibited the growth of 13 isolates of MRSA (Methicillin- Resistant Staphylococcus aureus) tested.

Keywords: Antibacterial activity; cytotoxicity; Phyllanthus columnaris; Phyllanthus gracilipes; Vero cell

RUJUKAN

Andrighetti-Frohner, C.R., Antonio, R.V., Creczynski-Pasa, T.B., Barardi, C.R.M. & Simões, C.M.O. 2003. Cytotoxicity and potential antiviral evaluation of violacein produced by Chromobacterium violaceum. Memõrias do Instituto Oswaldo Cruz 98(6): 843-848.

Bowornkiengkai, T., Tuchinda, P., Reutrakul, V., Pohmakotr, M. & Yoosook, C. 2009. Isolation and structure identification of compounds with anti-HIV-1 activity from anti-HIV-1 active Phyllanthus gracilipes (Euphorbiaceae). Proceedings of the 35^th. Congress on Science and Technology of Thailand. http://www.scisoc.or.th/stt/35/sec_c/paper/STT35_C3_C0099.pdf. 1-6.

Bauer, A.W., Kirby, W.M., Sherris, J.C. & Turck, M. 1966. Antibiotic susceptibility testing by a standardized disc method. American Journal of Clinical Pathology 459(4): 493-496.

Cheeke, P.R. 2000. Actual and potential applications of Yucca schidigera and Quillaja saponaria saponins in human and animal nutrition. Proceedings of the Phytochemical Society of Europe 45: 241-254.

Culvenor, C.C.J. & Fitzgerald, J.S. 1963. A field method for alkaloid screening of plants. Journal of Phamaceutical Sciences 52: 303-304.

de Padua, L.S. & Bunyapraphatsara, N. (ed.). 1999. Plant resources of South-East Asia. Bogor: Prosea Foundation.

Eloff, J.N. 1998. Which extractant should be used for the screening and isolation of antimicrobial components from plants? Journal of Enthnopharmacology 60(1): 1-8.

Freshney, R.I. 2005. Culture of Animal Cells: A Manual of Basic Technique. Ed. ke-5. Ottawa: Wiley-Liss.

Gertsch, J., Niowame, Gertsch-Roost, K. & Sticher, O. 2004. Phyllanthus piscatorum, ethnopharmacological studies on a women’s medicinal plant of the Yanomamї Amerindians. Journal of Ethnopharmacology 91(2-3): 181-188.

Hiramatsu, K., Cui, L., Kuroda, M., & Ito, T. 2001. The emergence and evolution of methicillin-resistant Staphylococcus aureus. Trends in Microbiology 9(10): 486-493.466

Holm-Nielsen, L.B. 1979. Comments on the distribution and evolution of the genus Phyllanthus (Euphorbiaceae). Dlm. Tropical Botany, disunting oleh Larsen, K. & Holm-Nielsen, L.B. New York: Academic Press.

Jamal, A.K., Yaacob, W.A. & Din, L.B. 2009. A chemical study on Phyllantus columnaris. European Journal of Scientific Research 28(1): 76-81.

Kaur, S., Michael, H., Arora, S., Haerkoenen, P.L. & Kumar, S. 2005. The in vitro cytotoxic and apoptotic activity of Triphala – an Indian herbal drug. Journal of Ethnopharmacology 97(1): 15-20.

Manríquez-Torres, J.J., Zúñiga-Estrada, A., González-Ledesma, M. & Torres-Valencia, J.M. 2007. The antibacterial metabolites and proacacipetalin from Acacia cochliacantha. J. Mex. Chem. Soc. 51(4): 228-231.

McRae, J., Yang, Q., Crawford, R. & Palombo, E. 2007. Review of the methods used for isolating pharmaceutical lead compounds from traditional medicinal plants. The Environmentalist 27(1): 165-174.

Mitra, R.L. & Jain, S.K. 1987. Concept of Phyllanthus niruri (Euphorbiaceae) in Indian floras. Bulletin of Botanical Survey of India 27: 161-176.

Oliveira, D.F., Pareira, A.C., Figueiredo, H.C.P., Carvalho, D.A., Silva, G., Nunes, A.S., Alves, D.S. & Carvalho, H.W.P. 2007. Antibacterial activity of plant extracts from Brazilian southeast region. Fitoterapia 78(2): 142-145.

Ramesh, N., Viswanathan, M.B., Selvi, V.T. & Lakshmanaperumalsamy, P. 2004. Antimicrobial and phytochemical studies on the leaves of Phyllanthus singampattiana from India. Medicinal Chemistry Research 13(6/7): 348-360.

Semple, S.J., Reynolds, G.D., O’Leary, M.C. & Flower, R.L.P. 1998. Screening of Australian medicinal plants for antiviral activity. Journal of Ethnopharmacology 60(2): 163-172.

Simes, J.J.H., Tracey, J.G., Webb, L.J. & Dunstan, W.J. 1959. Australian phytochemical survey. III. Saponins in Eastern Australian flowering plants. Bulletin No. 281. CSIRO: Melbourne, Australia.

Soetan, K.O., Oyekunle, M.A., Aiyelaagbe, O.O. & Fafunso, M. A. 2006. Evaluation of the antimicrobial activity of saponins extract of Sorghum bicolor. African Journal of Biotechnology 5(23): 2405-2407.

Sureban, S.M., Subramaniam, D., Rajendran P., Ramanujam, R.P., Dieckgraefe, B.K., Houchen, C.W. & Anant, S. 2006. Therapeutic effect of Phyllanthus species: induction of TNF-α-mediated apoptosis in HepG2 hepatocellular carcinoma cells. American Journal of Pharmacology and Toxicology 1(4): 65-71.

Turner, I.M. 1995. A catalogue of the vascular plants of Malaya. The Gardens’ Bulletin Singapore 47(1): 1-346.

Unander, D.W., Venkateswaran, P.S., Millman, I. Bryan, H.H. & Blumberg, B.S. 1990. Phyllanthus species: sources of new antiviral compounds. Dlm. Advances in new crops, disunting oleh Janick J. & Simon J.E. Portland: Timber Press.

Vani, T.M., Rajani, S. & Sarkar, S. & Shishoo, C.J. 1997. Antioxidant properties of the Ayurvedic formulation Triphala and its constituents. Int. J. Pharmacog. 35(5): 313-317.

Wall, M.E., Taylor, H. & Wani, M.C. 1987. Plant antitumor agents, 24. Rapid 9-KB assay. Journal of Natural Products 50(4): 764-766.

Yang, C.M., Cheng, H.Y., Lin, T.C., Chiang, L.C. & Lin, C.C. 2005. Acetone, ethanol and methanol extracts of Phyllanthus urinaria inhibit HSV-2 infection in vitro. Antiviral Research 67(1): 24-30.

*Pengarang untuk surat-menyurat; email: nazlina@ukm.my