Sains Malaysiana 40(5)(2011): 475–479

 

Survivin Expression in Uterine Cervical Exfoliative Cells as Diagnostic Test of Cervical Malignancy Process

(Pernyataan Survivin dalam Sel-sel Eksfoliatif Pangkal Rahim sebagai Ujian Diagnostik daripada Proses-proses Malignan Pangkal Rahim)

 

Zulham*

Department of Histology, Faculty of Medicine, University of Sumatera Utara

Jl. Dr. Mansur No. 5 Medan 20155 Sumatera Utara, Indonesia

 

Ahmad Aulia Jusuf

Department of Histology, Faculty of Medicine, University of Indonesia

Jl. Salemba Raya No. 6 Jakarta 10430, Indonesia

 

Junita Indarti

Dr. Cipto Mangunkusumo Hospital, Department of Obstetrics and Gynecology

Faculty of Medicine, University of Indonesia

Jl. P. Diponegoro No. 71 Jakarta 1043, Indonesia

 

Lisnawati

Dr. Cipto Mangunkusumo Hospital, Department of Anatomic Pathology

Faculty of Medicine, University of Indonesia

Jl. P. Diponegoro No. 71 Jakarta 10430, Indonesia

 

Diserahkan: 13 Ogos 2009 / Diterima: 11 October 2010

 

ABSTRACT

 

Papanicolaou test is a diagnostic test for uterine cervical cancer screening and routinely examined. It has limitations. A better technique is needed to identify true cervical malignancy process. Molecular cancer marker detection may have high the sensitivity and specificity in detecting cancer. Survivin, a marker candidate, is upregulated in many malignancy processes. Ninety women have joined in this cross sectional study by consecutive sampling. Survivin expression was examined by indirect immunoperoxidase method. It was predominantly found in metaplastic cells. Correlation between survivin expression and Papanicolaou test results was calculated by Fischer’s-exact test. Using Papanicolaou test result as an indicator for the presence of uterine cervical abnormalities, the performance indicators were calculated. Fischer’s-exact test showed that survivin expression was not significantly useful as cervical cancer molecular marker. Survivin expression of the uterine cervical exfoliative cells cannot be used as a diagnostic test for uterine cervical malignancy process.

 

Keywords: Diagnostic test; indirect immunoperoxidase; Papanicolaou test; survivin; uterine cervical cancer

 

ABSTRAK

 

Ujian Papanicolau adalah sebuah ujian diagnostik bagi pemeriksaan barah pangkal rahim (kanser serviks) dan dilakukan secara rutin. Ujian ini mempunyai beberapa had. Suatu teknik yang lebih baik diperlukan bagi mengenal pasti proses malignansi serviks yang sebenarnya. Survivin, satu calon penanda kanser, meningkat kadarnya dalam banyak proses malignansi. Sembilan puluh wanita telah menyertai kajian keratan lintang ini melalui pensampelan berturutan. Pernyataan survivin diperiksa dengan menggunakan kaedah imunoperoksidas tidak langsung. Korelasi antara ekspresi survivin dengan keputusan ujian Pap dikira dengan menggunakan ujian Fischer’s-exact. Seterusnya, dengan menggunakan keputusan yang diperoleh daripada ujian Papanicolau sebagai penunjuk adanya keganjilan serviks, penunjuk prestasi daripada immunositokimia daripada survivin boleh dikira. Ujian Fischer’s-exact menunjukkan bahawa ekspresi survivin tidak berguna secara penuh makna sebagai penanda kanser serviks secara molekular. Pernyataan survivin daripada sel-sel eksfoliatif serviks tidak boleh digunakan sebagai ujian diagnostik bagi proses malignan serviks.

 

Kata kunci: Imunoperoksidas tidak langsung; kanser pangkal rahim; survivin; ujian diagnostik; ujian Papanicolaou

 

RUJUKAN

 

Ashanuma, K., Tsuji, N., Endoh, T., Yagihashi, A. & Watanabe, N. 2004. Survivin enhances Fas ligand expression via up-regulation of specifity protein-1 mediated gene transcription in colon cancer cells. The Journal of Immunology 172: 3922-3929.

Branca, M., Giorgi, C., Santini, D., Bonito, L.D., Ciotti, M., Costa, S., Benedetto, A., Casolati, E.A., Favalli, C., Paba, P., Di Bonito, P., Mariani, L., Syrjänen, S., Bonifacio, D., Accardi, L., Zanconati, F. & Syrjänen, K. 2005. Survivin as a marker of cervical intraepithelial neoplasia and high-risk human papilloma virus and a predictor of virus clearance and prognosis in cervical cancer. American Journal of Clinical Pathology 124: 113-121.

Cannistra, S.A. & Niloff, J.M. 1996. Cancer of the uterine cervix. The New England Journal of Medicine 334: 1030-1038.

Carlson, M.W. 2006. Gene expression profiling and modeling of cervical cancer, Ph D Dissertation. Austin: Texas Univ.

Chen, Y., Miller, C., Mosher, R., Zhao, X., Deeds, J., Morissey, M., Bryant, B., Yang, D., Meyer, R., Cronin, F., Gostout, B.S., Smith-McCune, K. & Schlegel, R. 2003. Identification of cervical cancer markers by cDNA and tissue microarrays. Cancer Research 63: 1927-1935.

Frost, M., Jarboe, E.A., Orlicky, D., Gianani, R., Thompson, L.C., Enomoto, T. & Shroyer, K.R. 2002. Immunohistochemical localization of survivin in benign cervical mucosa, cervical dysplasia, and invasive squamous cell carcinoma. American Journal of Clinical Pathology 117: 738-744.

Geisler, J.P., Geisler, H.E., Miller, G.A., Wiemann, M.C., Zhou, Z., Miller, J. & Crabtree, W. 2002. Serum and molecular biological markers in cervical carcinoma and cervical carcinoma in situ. Continuing Medical Education Journal of Gynecologic Oncology 6(1): 81-85.

Li, F. 2003. Survivin study: What is the next wave? Journal of Cellular Physiology 197: 8-29.

Li, F. 2005. Role of survivin and its splice variants in tumorigenesis. British Journal of Cancer 92: 212-216.

Lu, B., Mu, Y., Cao, C., Zeng, F., Schneider, S., Tan, J., Price, J., Chen, J., Freeman, M. & Hallahan, D.E. 2004. Survivin as a therapeutic target for radiation sensitization in lung cancer. Cancer Research 64: 2840-2845.

Maddox, P., Sasieni, P., Szarewski, A., Anderson, M. & Hanby, A. 1999. Differential expression of keratins 10, 17, and 19 in normal cervical epithelium, cervical intraepithelial neoplasia, and cervical carcinoma. Journal of Clinical Pathology 52: 41-46.

Nakanishi, K., Kawai, T., Kumaki, F., Hiroi, S., Mukai, M. & Ikeda, E. 2003. Survivin expression in atypical adenomatous hyperplasia of the lung. American Journal of Clinical Pathology 120: 712-719.

Pusponegoro, H.D., Wirya, I.G.N.W., Pudjiadi, A.H., Bisanto, J. & Zulkarnain, S.Z. 2006. Uji diagnostik in Dasar-dasar Metodologi Penelitian Klinis. 2nd Ed. Sastroasmoro S, Ismael S. Eds. Jakarta: Sagung Seto.

Rodel, F., Hoffmann, J., Distel, D., Herrmann, M., Noisternig, T., Papadopoulos, T., Sauer, R. & Rödel, C. 2005. Survivin as a radioresistance factor, and prognostic and therapeutic target for radiotherapy in rectal cancer. Cancer Research 65: 4881-4887.

Salz, W., Eisenberm, D., Plescia, J., Garlick, D.S., Weiss, R.M., Wu, X-R. et al. 2005. A survivin gene signature predicts aggresive tumor behavior. Cancer Research 65: 3531-3534.

Tjindarbumi, D. & Mangunkusumo, R. 2002. Cancer in Indonesia, present and future. Japanese Journal of Clinical Oncology 32 suppl I: S17-21.

Vischioni, B., van der Valk, P., Span, S.W., Kruyt, F.A.E., Rodriguez, J.A. & Giaccone, G. 2004. Nuclear localization of survivin is a positive prognostic factor for survival in advanced non-small-cell lung cancer. Annals of Oncology 15: 1654-1660.

WHO. 2002. Cervical Cancer Screening in Developing Countries: Report of a WHO Consultation. Geneva: WHO.

Williams, G.H., Romanowski, P., Morris, L., Madine, M., Mills, A.D., Stoeber, K., Marr, J., Laskey, R.A. & Coleman, N. 1998. Improved cervical smears assessment using antibodies against proteins that regulate DNA replication. Proceedings of the National Academy of Sciences of the United States of America 95: 14932-14937.

 

*Pengarang untuk surat-menyurat; e-mail: dr_zulham@yahoo.com

 

 

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