Sains Malaysiana 41(12)(2012): 1587–1593
Penghasilan Hidrogen oleh Bakteria Rhodobacter sphaeroides NCIMB 8253
(Hydrogen Production via Rhodobacter sphaeroides NCIMB 8253)
Nor Zawanah Ab. Hamid, Jamaliah Md Jahim8, Nurina Anuar & Sahaid Khalid
Jabatan Kejuruteraan Kimia dan Proses, Fakulti Kejuruteraan & Alam Bina
Universiti Kebangsaan Malaysia,43600 Bangi,
Selangor, Malaysia
Diserahkan: 21 Oktober 2011 / Diterima: 03 Ogos 2012
ABSTRAK
Hidrogen merupakan sumber tenaga yang boleh diperbaharui dan efektif. Hidrogen boleh dihasilkan melalui fotofermentasi oleh bakteria ungu tanpa sulfur sepertiRhodobacter sphaeroides disebabkan kebolehan yang tinggi dalam menghasilkan hidrogen dalam keadaan anaerobik. Dalam kajian ini, bakteria dieram di dalam botol serum 100 mL dalam keadaan anaerobik. Kajian ini bertujuan untuk memaksimumkan penghasilan hidrogen olehRhodobacter sphaeroides NCIMB 8253 yang melibatkan kajian mengenai inokulum dalam keadaan aerobik dan anaerobik, kesan pH awal dan kesan keamatan cahaya terhadap penghasilan hidrogen. Daripada uji kaji yang dilakukan, didapati bahawa inokulum yang dieram pada keadaan anaerobik menghasilkan hidrogen tertinggi iaitu 220.5 mL/g substrat. pH 7 adalah pH yang paling sesuai digunakan untuk penghasilan hidrogen. Keamatan cahaya 5,000 lux adalah nilai optimum yang dapat memberikan penghasilan hidrogen tertinggi iaitu 80.21 mL hidrogen dengan hasil hidrogen 401.04 mL/g substrat. Penghasilan hidrogen didapati semakin menurun pada keamatan cahaya yang lebih tinggi daripada 5,000 lux.
Kata kunci: Bakteria fotosintetik; hidrogen, Rhodobacter sphaeroides
ABSTRACT
Hydrogen is a renewable source of energy and is effective.
Hydrogen can be produced by photofermentation by
purple non sulfur bacteria such as Rhodobacter sphaeroides, due to the high ability
to produce hydrogen in anaerobic conditions. In this study, bacteria were
incubated in 100 mL serum bottles in anaerobic conditions. The aims of this
study were to maximize the hydrogen production of Rhodobacter sphaeroides NCIMB 8253 which involves the study of the
inoculums in aerobic and anaerobic conditions and to determine the effect of
initial pH and the effect of light intensity on hydrogen production. From the
experiments, it was found that inoculums in anaerobic conditions produced the highest
hydrogen yield 220.5 mL/g substrate. pH 7 is the most
suitable pH for the hydrogen production. Light intensity of 5,000 lux is the
optimum value that would give the highest hydrogen production at 80.21 mL and
hydrogen yield 401.04 mL/g substrate. Hydrogen production was decline at light
intensities higher than 5,000 lux.
Keywords: Hydrogen; photosynthetic bacteria; Rhodobacter sphaeroides
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*Pengarang untuk surat-menyurat; email:jamal@eng.ukm.my