Sains
Malaysiana 41(3)(2012): 333–337
Chemical Components of Marine Sponge
Derived Fungus Fusarium proliferatum
Collected from Pulau Tinggi, Malaysia
(Komponen Kimia Kulat Fusarium
proliferatum diperolehi daripada Span Marin
di Perairan Pulau Tinggi, Malaysia)
Z. Mosadeghzad & G. H. N. Farahani
Department of Chemistry, Faculty of
Science, Islamic Azad University-Sharekord Branch
166 Shahrekord-Iran
Zuriati Zakaria
School of Chemical Sciences and Food
Technology, Faculty of Science and Technology
Universiti Kebangsaan Malaysia, 43600
Bangi, Selangor D.E. Malaysia
Asmat, A., Gires, U. & Wickneswari.
R.
School of Bio Sciences and Biotechnology,
Faculty of Science and Technology
Universiti Kebangsaan Malaysia, 43600
Bangi, Selangor D.E. Malaysia
Pittayakhajonwut, P.
National Centre for Genetic Engineering
and Biotechnology (BIOTEC)
National Science and Technology
Development Agency (NSTDA)
113, Paholyothin Road, Klong 1, Klong
Luang, Pathum Thani 12120 Thailand
Diserahkan: 26 April 2011 / Diterima: 13
September 2011
ABSTRACT
Marine fungus Fusarium
proliferatum derived from marine sponge collected
along Pulau Tinggi, Malaysia was cultivated on Potato Dextrose Broth and
incubated for 7 days at 30oC. The liquid cultures were then extracted
using ethyl acetate. The crude extract was investigated for its anti-microbial
activity and was passed through Sephadex column and the fractions were
collected. Reverse phase HPLC was used to monitor the component of crude
extract. HPLC guided purification of crude extract resulted in the isolation of
linoleic acid, 4-hydroxy phenethyl alcohol, 2,5-furandimethanol and adenosine.
Their structures were elucidated by spectroscopic methods.
Keywords: Antimicrobial activity; Fusarium proliferatum; marine fungi
ABSTRAK
Kulat marin Fusarium proliferatum yang diperolehi daripada span marin dikutip
di perairan Pulau Tinggi, Malaysia telah dieramkan pada Agar Potato Dextrose
(PDA) pada 30°C selama 7 hari. Kultur cecair diekstrak dengan pelarut etil
asetat. Ekstrak mentah dikaji untuk aktiviti antimikrob dan dimasukkan ke dalam
turus Sephadex dan fraksi dikumpulkan. HPLC fasa terbalik digunakan untuk
memantau komponen ekstrak mentah. Penulenan ekstrak mentah berpandukan HPLC
telah menghasilkan pemencilan asid linoleik, alcohol 4-hidroksifenetil,
2,5-furan dimetanol dan adenosine. Struktur telah ditentukan dengan kaedah
spektroskopi.
Kata kunci: Aktiviti antimikrob; Fusarium
proliferatum; kulat marin
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*Pengarang
untuk surat-menyurat; email: zmzad79@gmail.com
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