Sains
Malaysiana 42(1)(2013): 19–24
Isolation
of Lactobacillus from Periodontally Healthy Subjects and its
Antimicrobial
Activity
against Periodontal Pathogens
(Pemencilan Lactobacillus pada Subjek Gusi Sihat dan
Aktiviti Antimikrobnya
Terhadap Patogen Gusi)
Azizah Ahmad Fauzi & Zaleha Shafiei*
Department of Clinical Oral Biology, Faculty of Dentistry, Universiti
Kebangsaan Malaysia, Kuala Lumpur Campus, Jalan Raja Muda Abdul Aziz, 50300
Kuala Lumpur, Malaysia
Badiah Baharin & Nurulhuda Mohd.
Department of Periodontology, Faculty of Dentistry,
Universiti Kebangsaan Malaysia
Kuala Lumpur Campus, Jalan Raja Muda Abdul Aziz, 50300 Kuala
Lumpur, Malaysia
Diserahkan: 20 Disember 2011/Diterima: 28 Mei 2012
ABSTRACT
Bacteriocin or Bacteriocin like inhibitory substances (BLIS)
is a protein antibiotic that has a relatively narrow spectrum of killing
activity. It could potentially serve as a natural alternative to antibiotics in
reducing the development of multi-drug resistant bacteria. Antimicrobial
activity of the strains of Lactobacillus sp. isolated from healthy subjects (test strains) against Aggregatibacter
actinomycetemcomitans and other periodontal pathogens (indicator strains)
isolated from subgingival plaques of aggressive periodontitis patients were
determined by using deferred antagonism test and agar-well diffusion method.
Strains of Lactobacillus sp., Aggregatibacter
actinomycetemcomitans and black pigmented bacteria were selectively isolated
from TJA, TSBV and TSBA agars,
respectively. Mean diameter zone of inhibition of at least 10 mm was considered
as positive results for both methods. Out of 25 strains of Lactobacillus sp. screened, only eight test strains of Lactobacillus sp. showed the
specific antimicrobial activity against certain strains of indicator
periodontal pathogens during deferred antagonism test. However, out of eight
potential strains, only three strains, which were Lactobacillus sp.
strain S, Lactobacillus sp. strain V and Lactobacillus sp. strain
W consistently showed positive inhibitory activity against black pigmented
bacteria by deferred antagonism test and agar-well diffusion method. Therefore,
these three strains should be considered as potential BLIS producer
strains for further study.
Keywords: Antimicrobial activity; Lactobacillus sp.; periodontally healthy
subjects; periodontal pathogens
ABSTRAK
Bakteriosin atau bahan perencat seakan bakteriosin (BLIS)
merupakan protein antibiotik yang mempunyai medan aktiviti pembunuhan yang sempit. Ia berpotensi sebagai produk
alternatif antibiotik semula jadi dalam mengurangkan peningkatan bakteria yang
rentan terhadap pelbagai dadah. Aktiviti antimikrob strain Lactobacillus sp. yang dipencilkan daripada
subjek sihat (strain ujian) terhadap strain Aggregatibacter actinomycetemcomitans dan pathogen gusi yang lain
(strain penentu) yang dipencilkan daripada plak subgingiva pesakit
periodontitis agresif ditentukan dengan menggunakan kaedah ujian penentangan
tertangguh dan resapan telaga-agar. Strain Lactobacillus sp., Aggregatibacter actinomycetemcomitans dan
bakteria pigmen hitam telah dipencilkan secara terpilih pada agar TJA, TSBV dan TSBA. Purata diameter zon perencatan
yang lebih atau sama dengan 10 mm merupakan hasil
positif bagi kedua-dua kaedah. Sebanyak 25 strain Lactobacillus sp.
telah dikaji, namun hanya lapan strain ujian Lactobacillus sp. telah
menunjukkan aktiviti antimikrob yang spesifik terhadap strain pathogen gusi
tertentu semasa ujian penentangan tertangguh. Walau bagaimanapun, daripada
lapan strain yang berpotensi tersebut, hanya tiga strain iaitu Lactobacillus sp. strain S, Lactobacillus sp. strain V and Lactobacillus sp.
strain W secara tetap telah menunjukkan aktiviti perencatan yang positif
terhadap bakteria pigmen hitam menggunakan kaedah ujian penetangan tertangguh
dan resapan telaga-agar. Oleh yang demikian, ketiga-tiga strain tersebut
seharusnya dianggap berpotensi sebagai penghasil BLIS untuk
kajian lanjut.
Kata kunci: Aktiviti antimikrob; Lactobacillus sp.; patogen gusi; subjek dengan gusi sihat
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*Pengarang untuk surat-menyurat; email: zalehashafiei@yahoo.com
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