Sains Malaysiana 42(1)(2013): 53–58
Microbial Contamination Level of Water Supply System at the
Faculty of Dentistry, USIM, Malaysia
(Tahap Pelumusan Mikrob pada Sistem Bekalan Air di Fakulti
Pergigian, USIM, Malaysia)
Normaliza Ab. Malik*, Rohazila Mohd Hanafiah
& Wan Mohamad Nasi Wan Othman
Faculty
of Dentistry, University Sains Islam Malaysia (USIM), Aras 15-17, Menara B,
Persiaran
MPAJ, Jalan Pandan Utama, Pandan Indah, 55100 Kuala Lumpur, Malaysia
Diserahkan: 19 Disember 2011/Diterima: 21 Mei 2012
This study was to evaluate the microbial contamination level in
direct water supply at the Polyclinic, Faculty of Dentistry, USIM,
Malaysia. Water samples were collected randomly from water supplied via
the cup filler outlet of 20 dental units and 20 side water taps at Level 16 and
17 of Polyclinic, Faculty of Dentistry, USIM. All the
samples were placed and spread evenly on the surface of prepared agar media
(the nutrient agar) using the spread technique. Each sample consists of 0.5 mL
water. The microbial count was done using a magnifying glass and the total
number of bacteria concentration was reported as colony forming unit in 1 mL of
water (cfu/mL). In this study water from an aquarium was used as positive
control with 220 cfu/mL, while the distilled water taken from the CSSD was
used as negative control with no colony of microorganism. The study
demonstrated that there were low contamination before
the treatment that was beginning of the session in water supplied via the cup
filler outlet and side water tap from the sink with 2 cfu/mL. However, two cup fillers water and one side water taps from Polyclinic
level 17 showed a slightly higher bacterial colonies with 4 cfu/mL and 6 cfu/mL
of microbes. At the end of the session, result showed that higher bacterial
count from Polyclinic level 17 than Polyclinic level 16 with the highest
reading of 40 cfu/mL. The findings were considered low and the water was safe
for the dental procedures. The quality of water supplied at the Faculty of
Dentistry, USIM was within the limits recommended by the American Dental
Association, i.e. bacterial loads of not more than 200 cfu/mL for dental
procedures.
Keywords: Dental unit water system; filter system; microbial
contamination
ABSTRAK
Kajian ini telah dijalankan
bagi menilai tahap pelumusan mikrob di dalam bekalan air di Poliklinik, Fakulti
Pergigian, USIM. Sampel air telah diambil secara rawak
daripada air yang dibekalkan untuk cawan pengisi di 20 unit pergigian dan 20
sampel lagi daripada sumber air paip di sinki di unit pergigian di tingkat 16
dan 17 Poliklinik, Fakulti Pergigian, USIM. Semua
sampel telah diletakkan dan diratakan di atas permukaan media agar (agar
nutrien) menggunakan teknik merebak. Setiap sampel
terdiri daripada pencairan 0.5 mL. Kiraan mikrob telah
dilakukan dengan menggunakan kanta pembesar dan jumlah bakteria mengikut
kepekatan direkodkan sebagai unit membentuk koloni dalam 1 mL air (cfu/mL). Dalam kajian ini sampel air daripada akuarium telah digunakan sebagai kawalan
positif dengan 220 cfu/mL, manakala sampel air suling yang diambil dari Unit
Pensterilan digunakan sebagai kawalan negatif dengan tiada koloni
mikroorganisma. Kajian ini menunjukkan bahawa terdapat
pencemaran rendah sebelum rawatan pada permulaan sesi di dalam air yang
dibekalkan kepada cawan pengisi dan paip air dari sinki dengan 2 cfu/mL. Walau bagaimanapun, dua air daripada sumber cawan pengisi dan satu air paip
daripada Poliklinik 17 telah menunjukkan bacaan koloni bakteria yang lebih
tinggi sedikit dengan 4 cfu/mL dan 6 cfu/mL mikrob. Pada akhir sesi, kajian
menunjukkan bahawa kiraan bakteria yang lebih tinggi di Poliklinik 17 daripada
Poliklinik 16 dengan bacaan tertinggi 40 cfu/mL. Penemuan ini
masih dianggap rendah dan air adalah selamat untuk prosedur rawatan pergigian. Oleh yang demikian, kualiti air yang dibekalkan di Fakulti Pergigian, USIM adalah dalam had yang disyorkan oleh Persatuan Pergigian Amerika,
iaitu bacaan bakteria tidak melebihi daripada 200 cfu/mL untuk prosedur rawatan
pergigian.
Keywords: Pencemaran
mikrobial; sistem air unit pergigian; sistem penapis
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*Pengarang
untuk surat-menyurat; email: liza_arie2004@yahoo.com
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