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Sains Malaysiana 42(5)(2013):
625–628
In Vitro Antidiabetic
Activity of Nisamalaki Churna
(Aktiviti Antidiabetik In Vitro Nisamalaki Churna)
B.S. Ashok Kumar* & N.K. Manjunath
Department of Pharmacognosy, Sri K.V.
College of Pharmacy, Chickballapur, Karnataka, India
Saleemulla Khan
Department of Pharmacognosy, Manipal College
of Pharmaceutical Sciences, Manipal, Karnataka, India
Gopi
Setty Saran
Department
of Pharmaceutics, Sri K.V.College of Pharmacy, Chickballapur, Karnataka, India
R.
Nandeesh
Department
of Pharmacognosy, SreeSiddaganga College of Pharmacy, Tumkur, Karnataka, India
Diserahkan:
27 Mac 2012/Diterima: 2 Oktober 2012
ABSTRACT
Nisamalaki Churna is an Ayurveda formulation used for diabetes, which consists of
amalaki and haridra. In vitro antidiabetic screening of Nisamalaki
Churna by α-amylase
inhibition was carried out by starch iodine method, initrosalicylic acid method (DNSA)
and effect on normal rats blood sugar level was studied. Nisamalaki Churna
shows potent α-amylase inhibition IC50 89.44 μg/mL by starch iodine method
and IC50100.0
μg/mL by DNS method. Nisamalaki Churna showed potent decrease in blood glucose
level in normal rats.
Keywords: α-amylase inhibition; antidiabetic screening;
Nisamalaki Churna
ABSTRAK
Nisamalaki Churna adalah formula Ayurveda untuk diabetis yang mengandungi
amalaki dan haridra. Penyaringan antidiabetis in vitro Nisamalaki Churna telah dijalankan. Kesan Nisamalaki Churna terhadap perencatan α-amilase
telah dikaji menggunakan kaedah iodin kanji. Dengan menggunakan kaedah
asid dinitrosalisilik (DNSA) kesan terhadap aras gula dalam darah tikus biasa telah dijalankan. Nisamalaki
Churna menunjukkan kesan perencatan alfa amilase yang kuat, IC50 89.44 μg/mL
melalui kaedah iodin kanji dan IC50100.0 μg/mL DNS. Nisamalaki
Churna menunjukkan penurunan aras glukos yang kuat
dalam tikus biasa.
Kata kunci: Nisamalaki Churna; penyaringan
antidiabetis; perencatan α-amylase
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*Pengarang
untuk surat-menyurat; email: ashok4vani@gmail.com
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