Sains
Malaysiana 42(5)(2013): 629–633
Characterization of Fusarium semitectum Isolates
from Vegetable Fruits
(Pencirian Pencilan Fusarium semitectum daripada Buahan
Sayuran)
Z.
Latiffah*, M.S. Nurul Huda & T.M.A. Tengku Ahmad Akram
School
of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia
Diserahkan:
1 Disember 2011/Diterima: 14 November 2012
ABSTRACT
Fusarium semitectum is a widespread
species occurring in various types of substrate. Fifteen F. semitectum isolates were recovered from several types of vegetable fruits showing fruit
rot symptoms, namely long bean (Vigna sesquilpedalis), okra (Abelmoschus esculentus), loofa (Luffa acutangula), bitter gourd (Momordica charantia), cucumber
(Cucumis sativus) and green chilli (Capsicum annum). The identification
of the F. semitectum isolates was based on morphological characteristics
of macroconidia and microconidia, presence of mesoconidia and colony
pigmentation. The isolates were then characterized using vegetative
compatibility group (VCG) and sequencing of translation
elongation factor-1α gene (TEF-1α). From VCG analysis,
11 isolates were assigned to six VCGs and another four isolates
were self-incompatible. The results of the phylogenetic analysis using TEF-1α
sequences showed that the isolates were phyogenetically distinct although the
morphological characteristics were very similar and generally the grouping of
the isolates was not according to the host. Both VCG and
phylogenetic analyses indicated that F. semitectum isolates from
vegetable fruits were highly variable which suggested that the isolates belong
to a species complex.
Keywords: Fusarium
semitectum; TEF-1α; VCG; vegetable fruits
ABSTRAK
Fusarium semitectum merupakan spesies yang tersebar dengan meluas, menduduki pelbagai jenis
substrat. Lima belas pencilan F. semitectum telah diperoleh daripada beberapa jenis buahan sayuran yang menunjukkan gejala
reput buah iaitu kacang panjang (Vigna sesquilpedalis), okra (Abelmoschus esculentus), petola (Luffa acutangula), peria (Momordica charantia), timun (Cucumis sativus) dan cili hijau(Capsicum
annum). Pencilan F.
semitectum telah dikenal pasti berdasarkan ciri-ciri morfologi
makrokonidia, mikrokonidia, kehadiran mesokonidia and pigmentasi koloni. Pencilan tersebut kemudiannya dicirikan menggunakan kumpulan keserasian
vegetatif (VCG) dan penjujukan gen faktor pemanjangan translasi (TEF-1α).
Daripada analisis VCG, 11 pencilan diletakkan ke dalam
enam kumpulan VCG dan empat pencilan yang selebihnya menunjukkan
ketidakserasian sendiri. Keputusan analisis filogenetik
menggunakan jujukan TEF-1α menunjukkan pencilan
tersebut berbeza secara filogenetik walaupun ciri-ciri morfologinya adalah
serupa dan secara amnya pencilan tersebut tidak dikumpulkan mengikut perumah. Kedua-dua analisis VCG dan filogenetik menunjukkan
pencilan F. semitectum daripada buahan sayuran mempunyai variasi yang
tinggi yang dicadangkan pencilan tersebut tergolong dalam satu kompleks spesies.
Kata kunci: Buahan sayuran; Fusarium semitectum; TEF-1α; VCG
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*Pengarang
untuk surat-meneyurat; email: Lfah@usm.my
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