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Sains Malaysiana 42(5)(2013):
635–642
Effects of GnRHa on Plasma Sex Steroid Hormones of River Catfish Hemibagrus nemurus(Valenciennes 1840)
(Kesan GnRHa Terhadap Hormon Seks Steroid Plasma Ikan Baung Hemibagrus nemurus(Valenciennes 1840))
FatimatAdenike Adebiyi, S itiS haporSiraj*, S harrAzniHarmin & Annie Christianus
Department
of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia
43400
UPM Serdang, Selangor, Malaysia
Diserahkan: 12 Mac 2012/Diterima:
4 Oktober 2012
ABSTRACT
The effect of gonadotropin releasing hormone analogue (GnRHa) on plasma sex steroid hormones of river catfish Hemibagrus nemurus was studied. Enzyme linked immunosorbent assay (ELISA)
was used to measure the hormones. The fish were treated with saline (control)
and GnRHa at various doses of 5 μg/kg, 20
μg/kg and 50 μg/kg body weight (BW)
of fish. Blood samples were collected at 0, 6, 12 and 24 h post hormone
administration. The results showed that GnRHa elevated the plasma sex steroid hormones even at a low dose of 5 μg/kg.
Significant increase (p< 0.05) in plasma sex steroid levels were observed with 20 μg/kg and 50 μg/kg BW GnRHa treatments and a dose of 5 μg/kg BW GnRHa produced a slow response to steroidogenesis.
Treatment with 50 μg/kg BW GnRHa produced the best result. The highest level of testosterone,
11-ketotestosterone and 17β-estradiol were observed at 24 h for all
treatments. The results indicated that GnRHa increased steroid production in the plasma of H. nemurus.
Therefore, it can be used as an inducing agent for the control of reproduction
in H. nemurus.
Keywords: Gonadotropin releasing hormone analogue (GnRHa); Hemibagrus nemurus; testosterone; 11-ketotestosterone;
17β-estradiol
ABSTRAK
Kesan analog hormon lepasan gonadotropin (GnRHa) terhadap hormon seks steroid plasma ikan baungHemibagrus nemurus telah dikaji. Enzim berkaitan imunosorben asei (ELISA) telah digunakan untuk mengukur hormon. Ikan telah dirawat dengan salina (kawalan) dan GnRHa pada pelbagai dos 5
μg/kg, 20 μg/kg dan 50 μg/kg berat badan (BW) ikan. Sampel darah telah dikumpul pada 0, 6, 12 dan 24 jam selepas suntikan hormon. Keputusan menunjukkan bahawa GnRHa meningkatkan hormon seks steroid plasma walaupun pada dos yang rendah, 5 μg/kg. Peningkatan yang signifikan (p< 0.05) dalam tahap seks steroid plasma telah diperhatikan pada rawatan 20 μg/kg dan 50 μg/kg BW GnRHa sementara dos 5 μg/kg BW GnRHa menghasilkan tindak balas yang perlahan untuk steroidogenesis. Rawatan GnRHa pada dos
50 μg/kg BW menunjukkan hasil yang terbaik. Tahap tertinggi testosteron, 11-ketotestosterone dan-estradiol
17β telah diperhatikan pada 24 jam untuk semua rawatan. Keputusan menunjukkan bahawa GnRHa meningkatkan pengeluaran steroid dalam plasma H. nemurus. Oleh itu, ia boleh digunakan sebagai ejen pendorong untuk kawalan pembiakanH. nemurus.
Kata kunci: Analog hormon lepasan gonadotropin (GnRHa); Hemibagrus nemurus; testosteron; 11-ketotestosterone; 17β-estradiol
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*Pengarang untuk surat-menyurat; email: shapor@putra.upm.edu.my |
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