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Sains Malaysiana 42(6)(2013): 707–713
Psidium guajava Fruit Peel Extract Reduces Oxidative Stress of Pancreas in Streptozotocin-induced Diabetic Rats (Ekstrak Kulit Buah Psidium guajava Menurunkan Tekanan
Oksidatif Pankreas
pada Tikus Diabetes Aruhan Streptozotosin)
Siti Balkis Budin*, Hawa Ismail
& P ek LianChong
Biomedical Science Program, School of Diagnostic and Applied
Health Sciences
Faculty of Health Sciences, Universiti Kebangsaan Malaysia
Jalan Raja Muda Abdul Aziz, 50300, Kuala LumpurMalaysia
Diserahkan: 8 Ogos 2012/Diterima: 15 November 2012
ABSTRACT
Abundant natural products with medicinal properties have been used
as food and traditional medicine for diabetes mellitus all over the world. Psidium guajava fruit from the family of
Myrtaceae has gained attention for its antioxidant potential. This study was
conducted to determine the effects of P. guajava fruit peel aqueous
extract on oxidative stress of pancreas in streptozotocin-induced (45 mg/kg)
diabetic rats. Diabetic rats were administered with 400 mg/kg of aqueous
extract of P. guajava fruit peel daily for 28 days duration. The results
showed that diabetic rats supplemented with P. guajava extract did not
cause significant difference in blood glucose level (p>0.05) as compared
with diabetic rats alone. For oxidative stress evaluation, malondialdehyde (MDA)
and protein carbonyl level were significantly lower and the activity of
superoxide dismutase (SOD) and glutathione (GSH)
level were significantly higher (p<0.05) in P. guajava supplemented
rats compared with non-supplemented diabetic rats. However, histological
observation showed that supplementation of P. guajava extract did not give protective effects towards
alterations in pancreas histology in diabetic rats. The findings suggested that
aqueous extract of P. guajava fruit peel supplementation has the ability to
reduce oxidative stress in pancreas of diabetic rats and may play a role in
reducing the development of diabetic complications.
Keywords: Antioxidant; histopathology; lipid peroxidation; Psidium guajava; type 1
diabetes
ABSTRAK
Pelbagai bahan semula jadi yang dilengkapi
ciri-ciri perubatan telah digunakan sebagai sumber makanan serta ubat tradisi
untuk pesakit diabetes melitus di seluruh dunia. Kulit buah Psidium guajava yang berasal daripada keluarga Myrtaceae telah
mendapat perhatian penyelidik untuk mengetahui potensi antioksidannya. Penyelidikan ini dijalankan untuk menentukan kesan ekstrak akueus
kulit buah P. guajava terhadap tekanan
oksidatif pankreas pada tikus diabetes aruhan streptozotosin (45 mg/kg). Tikus diabetes telah diberi 400 mg/kg ekstrak akueus kulit
buah P. guajava setiap hari untuk 28
hari. Hasil kajian menunjukkan bahawa tiada perbezaan signifikan (p>0.05)
pada aras glukosa darah tikus diabetes yang menerima
suplementasi P. guajava berbanding tikus diabetes yang tidak menerima
suplementasi. Untuk penilaian tekanan oksidatif, tikus diabetes yang
disuplementasi P. guajava mempunyai aras malondialdehid (MDA) dan karbonil protein (PC)
yang lebih rendah serta aktiviti superoksida dismutase (SOD)
dan aras glutation (GSH) yang lebih tinggi secara
signifikan (p<0.05) berbanding tikus diabetes tidak disuplementasi. Walau bagaimanapun, analisis histologi menunjukkan berlaku
kerosakan pankreas pada kumpulan tikus diabetes tidak dapat dibaiki dengan
supplementasi P. guajava. Hasil kajian telah menunjukkan bahawa ekstrak akueus kulit buah P. guajava berpotensi menurunkan tekanan
oksidatif tisu pankreas pada tikus diabetes dan berkemungkinan dapat berperanan
dalam mengurangkan komplikasi diabetes.
Kata kunci: Antioksidan; diabetes jenis 1;
histopatologi; peroksidasi lipid; Psidium guajava
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*Pengarang untuk surat-menyurat; email: balkis6466@yahoo.com.my
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