Sains Malaysiana 42(7)(2013):
943–948
A Qualitative Analysis of Litsea fulvaEssential Oils Using Comprehensive
Two-dimensional Gas Chromatography Coupled with
Time-of-Flight Mass
Spectrometry
(Penganalisan Kualitatif dalam Minyak Pati Litsea fulvaMelalui Gas Kromatografi Dua Dimensi
Berganding dengan Spektrometer Jisim Masa-Terbang (TOF/MS))
Khong Heng Yen*
School of Chemistry and Environmental Studies, Faculty of Applied
Sciences
Universiti Teknologi MARA, Jalan Meranek, 94300 Kota Samarahan, Sarawak
Malaysia
Laily B Din, Norzamzurina Ismail & Mohd Ambar Yarmo
School of Chemical Sciences & Food Technology, Faculty of
Science and Technology
Universiti Kebangsaan Malaysia, 43400 Bangi,
Selangor, D.E. Malaysia
Zuriati Zakaria
Malaysia Japan International Institute of Technology, Universiti Teknologi Malaysia
54100 Kuala Lumpur, Malaysia
Nor Hadiani Ismail
Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam,
Selangor, D.E. Malaysia
Diserahkan: 27 Jun
2012/Diterima: 15 November 2012
ABSTRACT
A qualitative analysis of the individual compounds in Litsea fulva (locally
known as ‘Medang’) essential oils was
performed by comprehensive two-dimensional gas chromatography (GC × GC)
coupled with time-of-flight mass spectrometer (TOF/MS)
for the identification of the resolved peaks. Litsea fulva essential oil was found to contain 98
identifiable peaks with 32 compounds were identified with good matches. These
compounds identified included 30 hydrocarbons, 22 alcohols, five acids, 16
ketones, five aldehydes, 12 esters, six ethers and two other compounds. The L. fulva leaf oil contained alcohols and ethers, with
34.09% and 24.38%, respectively. The major components of these oils were cis-Z-α-bisabolene epoxide (9.51%), trans-Z-α-bisabolene epoxide (8.36%), C13H20O2 (7.39%), longipinocarvone (5.68%), τ-Cadinol (4.24%), C15H24O
(4.98%) and α-cadinol (3.95%). The study
also showed that the comprehensive two-dimensional gas chromatography (GC × GC)
is a better and more powerful separation tool in GC and
an identification tool for analyzing complex volatile oils compared with the
one-dimensional GC.
Keywords: Comprehensive two-dimensional gas chromatography;
essential oils; Litsea fulva; time-of-flight mass spectrometer
ABSTRAK
Analisis kualitatif setiap sebatian dalam minyak pati Litsea fulva (dikenali sebagai‘Medang’) dilakukan melalui kromatografi gas dua dimensi berganding dengan spektrometer jisim masa-terbang (TOF/MS) untuk pengecaman puncak-puncak sebatian yang terlerai. Minyak patiLitsea fulva didapati mengandungi 98 puncak dengan 32 daripadanya dapat dicamkan sebatiannya. Sebatian yang telah dicamkan termasuklah 30 hidrokarbon, 22 alkohol, lima asid, 16 keton, lima aldehid, 12 ester, enam eter dan dua sebatian lain. Kelimpahan yang paling tinggi dalam minyak pati daunLitsea fulva ialah alkohol dan ester, masing-masing 34.09% dan 24.38%. Komponen utama minyak ini ialah cis-Z-α-bisabolena epoksida (9.51%), trans-Z-α-bisabolena eposida (8.36%), C13H20O2 (7.39%), longipinokarvon (5.68%), τ-kadinol (4.24%), C15H24O (4.98%) dan α-kadinol (3.95%). Kajian ini juga menunjukkan kromatografi gas dua dimensi komprehensif adalah alat yang lebih baik untuk pemisahan dalam GC dan alat pengecaman untuk analisis minyak meruap yang kompleks berbanding GC satu dimensi.
Kata kunci: Kromatografi gas dua dimensi komprehensif; Litsea fulva; minyak pati; spektrometer jisim masa-terbang
RUJUKAN
Ahmad, F.B., Jantan,
I.B., Bakar, B.A. & Ahmad, A.S.B. 2005. A comparative study of the composition of the
leaf oils of three Litsea species from
Borneo. Journal of Essential Oil Research 17(3): 323-326.
Argent, G., Saridan,
A., Camphell, E.J.F., Wilkie,
P. & Fairweather, G. 1997. Manual of the larger and more important non dipterocarp Trees of Central Kalimantan Indonesia.
Vol. 1. The Forest Research Institute of Samarinda,
Indonesia. p. 300.
Bighelli, A., Muselli, A.,
Casanova, J., Tam, N.T., Van Anh, V. & Bessiere, J.M. 2005. Chemical variability of Litsea cubebaleaf oil from Vietnam. Journal of
Essential Oil Research 17(1): 86-88.
Choi, E.M. & Hwang, J.K. 2004. Effect of methanolic extract and fractions from Litsea cubebabark on the production of inflammatory
mediators in RAW 264.7 cells. Fitoterapia 75: 141-148.
Dalluge, J., van Stee, L.L.P., Xu, X.,
Williams, J., Beens, J., Vreuls,
R.J.J. & Brinkman, U.A.Th. 2002. Unravelling the composition of very complex samples by
comprehensive gas chromatography coupled to time-of-flight mass spectrometry
Cigarette smoke. Journal of Chromatography A 974: 169.
Karim, Y.
& Adirukmi, N.S. 1991. Unusual aroma compounds
from forest trees and plants. In Medicinal Products from Tropical Rain
Forest, edited by Knozirah, S., Azizol, A.K. & Abdul, R.M.A. Kuala Lumpur: Forest
Research Institute Malaysia. p. 401.
Lyth, G. & Charles, S.
1998. Essential oils. Available at www.quinessence.com
Marriot, P. & Shellie, R. 2002. Principle
and applications in a comprehensive two-dimensional gas chromatography. Trend
Analytical Chemistry 21: 573.
Valverdu, C.,
Vila, R., Cruz, S.M., Caceres, A. & Carligueral,
S. 2005. Composition of the essential oil from leaves of Litsea guatemalensis. Flavour and Fragrance Journal 20(4): 415-418.
Wu, J., Lu, X., Tang, W., Kong, H., Zhou, S. & Xu, G. 2004. Application of comprehensive
two-dimensional gas chromatography-time-of-flight mass spectrometry in the
analysis of volatile oil of traditional Chinese medicines. Journal of
Chromatography A 1034: 199-205.
*Pengarang untuk surat-menyurat; email: khonghy@sarawak.uitm.edu.my
|