Sains Malaysiana 43(1)(2014): 31–36

 

Insecticide Susceptibility of Bemisia tabaci to Karate® and Cydim Super® and

its Associated Carboxylesterase Activity

(Kerentanan Bemisia tabaci terhadap Racun Serangga Karate® dan Cydim Super® serta Kaitannya terhadap Aktiviti Karboksilesterase)

 

S.W. AVICOR1*, V.Y. EZIAH2, E.O. OWUSU3& M.F.F. WAJIDI1

 

1Molecular Entomology Research Group (MERG), School of Distance Education

Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia

 

2Department of Crop Science, College of Agriculture and Consumer Sciences

University of Ghana, Legon, Ghana

 

3Department of Animal Biology and Conservation Science (DABCS), University of Ghana,

Legon, Ghana

 

Diserahkan: 6 Januari 2013/Diterima: 23 April 2013

 

ABSTRACT

Bemisia tabaci is a pest of several economic crops worldwide and is mostly managed in Ghana by farmers through the use of insecticides. However, vegetable farmers have recently expressed concerns about the susceptibility of  B. tabaci to insecticides. Hence, the susceptibility status of field populations of B. tabaci on cassava, okra and tomato in vegetable growing sites in Accra to two commonly used insecticides, Karate® 2.5 EC (λ-cyhalothrin) and Cydim Super® (36 g Cypermethrin + 400 g Dimethoate per litre), using a modified dipping method and their carboxylesterase activity levels were assessed. The LC50 values of the B. tabaci populations to the insecticides were low and ranged from 0.12-0.55 mL/L to 0.07-0.36 mL/L for Karate® and Cydim Super®, respectively. There was a wide variation in carboxylesterase activity levels of the insect populations with high levels recorded in some of the populations. The elevated activity levels could negatively impact on future whitefly management methods. This study provides baseline information on the insecticide resistance status and carboxylesterase levels of whitefly populations in these areas and for monitoring future insecticide resistance development.

 

Keywords: Bemisia tabaci; carboxylesterase; insecticides; susceptibility; vegetables

 

ABSTRAK

Bemisia tabaci ialah salah satu serangga perosak kepada beberapa tanaman ekonomi di seluruh dunia dan kebanyakannya berjaya dikawal di Ghana oleh para petani melalui penggunaan racun serangga. Walau bagaimanapun, para petani menyatakan kebimbangan tentang kerentanan B. tabaci terhadap racun serangga pada masa hadapan. Oleh itu, status kerentanan populasi bidang B. tabaci pada ubi kayu, bendi dan tomato di tapak semaian di Accra bagi dua racun serangga biasa digunakan, Karate® 2.5 EC (λ-cyhalothrin) dan Cydim Super® (36 g Cypermethrin + 400 g Dimethoate setiap liter), menggunakan kaedah pencelupan diubah suai dan tahap aktiviti karboksilesterase mereka telah diuji. Nilai LC50 populasi B. tabaci bagi racun serangga didapati rendah, masing masing antara 0.12-0.55 mL/L dan 0.07-0.36 mL/L untuk Karate® dan Cydim Super®. Terdapat satu variasi luas bagi tahap aktiviti karboksilesterase bilangan serangga dengan tahap paling tinggi direkodkan dalam beberapa populasi. Ketinggian tahap aktiviti ini menggambarkan impak negatif pada masa hadapan tentang cara pengurusan bena putih. Kajian ini memberikan maklumat asas tentang kerentanan racun serangga dan karboksilesterase oleh populasi bena putih di kawasan ini bertujuan untuk memantau keboleh-rentanan racun serangga pada masa akan datang.

 

Kata kunci: Bemisia tabaci; karboksilesterase; kerentanan; racun serangga; sayur-sayuran

RUJUKAN

Abbott, W.S. 1925. A method of computing the effectiveness of an insecticide. J. Econ. Entomol. 18(2): 265-267.

Ahmad, M. 2007. Potentiation/antagonism of pyrethroids with organophosphate insecticides in Bemisia tabaci (Homoptera: Aleyrodidae). J. Econ. Entomol. 100(3): 886-893.

Ahmad, M., Arif, M.I. & Naveed, M. 2010. Dynamics of resistance to organophosphate and carbamate insecticides in the cotton whitefly Bemisia tabaci (Hemiptera:Aleyrodidae) from Pakistan. J. Pest Sci. 83(4): 409-420.

Alon, M., Alon, F., Nauen, R. & Morin, S. 2008. Organophosphates’ resistance in the B-biotype of Bemisia tabaci (Hemiptera:Aleyrodidae) is associated with a point mutation in an ace 1-type acetylcholinesterase and overexpression of carboxylesterase. Insect Biochem. Mol. Biol. 38(10): 940-949.

Bacci, L., Crespo, A.L.B., Galvan, T.L., Pereira, E.J.G., Picanço, M.C., Silva, G.A. & Chediak, M. 2007. Toxicity of insecticides to the sweetpotato whitefly (Hemiptera: Aleyrodidae) and its natural enemies. Pest Manag. Sci. 63(7): 699-706.

Boykin, L.M., Shatters, R.G. Jr., Rosell, R.C., McKenzie, C.L., Bagnall, R.A., De Barro, P. & Frohlich, D.R. 2007. Global relationships of Bemisia tabaci (Hemiptera: Aleyrodidae) revealed using Bayesian analysis of mitochondrial COI DNA sequences. Mol. Phylogenet. Evol. 44(3): 1306-1319.

Castle, S.J., Prabhaker, N., Henneberry, T.J. & Toscano, N.C. 2009. Host plant influence on susceptibility of Bemisia tabaci (Hemiptera: Aleyrodidae) to insecticides. Bull. Entomol. Res. 99(3): 263-273.

De Barro, P.J., Liu, S-S., Boykin, L.M. & Dinsdale, A.B. 2011. Bemisia tabaci: A statement of species status. Annu. Rev. Entomol. 56: 1-19.

Dinsdale, A., Cook, L., Riginos, C., Buckley, Y.M. & De Barro, P. 2010. Refined global analysis of Bemisia tabaci (Hemiptera: Sternorrhyncha: Aleyrodoidea: Aleyrodidae) mitochondrial cytochrome oxidase 1 to identify species level genetic boundaries. Ann. Entomol. Soc. Am. 103(2): 196-208.

Dittrich, V., Ernst, G.H., Ruesch, O. & Uk, S. 1990. Resistance mechanisms in sweetpotato whitefly (Homoptera: Aleyrodidae) populations from Sudan, Turkey, Guatemala, and Nicaragua. J. Econ. Entomol. 83(5): 1665-1670.

Ellsworth, P.C. & Martinez-Carrillo, J.L. 2001. IPM for Bemisia tabaci: A case study from North America. Crop Prot. 20(9): 853-869.

Environmental Protection Agency (EPA) 2006. Probit analysis LC50 software program version 1.5. http://www.epa.gov/ nerleerd/stat2.htm.

ffrench-Constant, R.H., Daborn, P.J. & Goff, G.L. 2004. The genetics and genomics of insecticide resistance. Trends Genet. 20(3): 163-170.

Gadelseed, A.M.A. 2000. Biotype–host association and distribution of adult Bemisia tabaci (Genn) (Homoptera: Aleyrodidae) on cassava, tomato, garden eggs, okra and coral plant. M.Phil thesis. ARPPIS, University of Ghana, Legon, Ghana (unpublished).

Global Invasive Species Database (GISD) 2012. Bemisia tabaci. http://www.issg.org/database/species/ecology. asp?si=106&fr=1&sts=&lang=EN [Accessed 22 July 2012].

Gonzalez-Zamora, J.E., Leira, D., Bellido, M.J. & Avilla, C. 2004. Evaluation of the effect of different insecticides on the survival and capacity of Eretmocerus mundus Mercet to control Bemisia tabaci (Gennadius) populations. Crop Prot. 23(7): 611-618.

Horowitz, A.R., Denholm, I. & Morin, S. 2007. Resistance to insecticides in the TYLCV vector, Bemisia tabaci. In Tomato Yellow Leaf Curl Virus Disease, edited by Czosnek, H. Dordrecht (Netherlands): Springer. pp. 305-325.

Houndete, T.A., K´etoh, G.K., Hema, O.S.A., Brevault, T., Glithob, I.A. & Martin, T. 2010. Insecticide resistance in field populations of Bemisia tabaci (Hemiptera: Aleyrodidae) in West Africa. Pest Manag. Sci. 66(11): 1181-1185.

Li, L-L., Xue, M., Ren, G.W., Li, Q.L., Zhang, Q.C. & Wang, H.T. 2009. Toxicity of several insecticides to different instars nymphs of Bemisia tabaci and the repellent actions to the adults. Acta Phytophylacica Sinica 36(4): 359-365.

Li, X., Schuler, M.A. & Berenbaum, M.R. 2007. Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics. Annu. Rev. Entomol. 52: 231-253.

Liang, P., Cui, J-Z., Yang, X-Q. & Gao, X-W. 2007. Effects of host plants on insecticide susceptibility and carboxylesterase activity in Bemisia tabaci biotype B and greenhouse whitefly, Trialeurodes vaporariorum. Pest Manag. Sci. 63(4): 365-371.

Obeng-Ofori, D. 2007. Major arthropod pests of root, tuber crops and plantains. In Major Pests of Food and Selected Fruit and Industrial Crops in West Africa, edited by Obeng-Ofori, D. Accra (Ghana): City Publishers. pp. 59-75.

Oliveira, M.R.V., Henneberry, T.J. & Anderson, P. 2001. History, current status, and collaborative research projects for Bemisia tabaci. Crop Prot. 20(9): 709-723.

Omondi, A.B. 2003. Characterisation of Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) biotypes associated with some selected crops in southern Ghana. M.Phil. thesis, ARPPIS, University of Ghana, Legon (unpublished).

Omondi, A.B., Obeng-Ofori, D., Kyerematen, R. & Danquah, E.Y. 2005. Host preference and suitability of some selected crops for two biotypes of Bemisia tabaci in Ghana. Entomol. Exp. Appl. 115(3): 393-400.

Ortiz, O. 2006. Impact Assessment. Tropical Whitefly Project (TWFP) Progress Report. Impact Evaluation (August 2006). http://www.spipm.cgiar.org/c/document_library/ get_file?p_l_id=17831&folderId=18530&name=DLFE-103. pdf. (Accessed 11 November 2011).

Owusu, E.O., Kim, C. & Horiike, M. 1995. Susceptibility of cotton aphid, Aphis gossypii glover (Homoptera:Aphididae) clones to dichlorvos and its relationship to activity levels of some esterases. Res. Rep. (Agric.) Kochi Univ. 44: 59-67.

Palumbo, J.C., Horowitz, A.R. & Prabhaker, N. 2001. Insecticidal control and resistance management for Bemisia tabaci. Crop Prot. 20(9): 739-765.

Perring, T.M. 2001. The Bemisia tabaci species complex. Crop Prot. 20(9): 725-737.

Prabhaker, N., Toscano, N.C. & Henneberry, T.J. 1998. Evaluation of insecticide rotations and mixtures as resistance management strategies for Bemisia argentifolii (Homoptera: Aleyrodidae). J. Econ. Entomol. 91(4): 820-826.

Roditakis, E., Grispou, M., Morou, E., Kristoffersen, J.B., Roditakis, N., Nauen, R., Vontas, J. & Tsagkarakou, A. 2009. Current status of insecticide resistance in Q biotype Bemisia tabaci populations from Crete. Pest Manag. Sci. 65(3): 313-322.

Salem, H.A., Sabry, E.K.H. & Aref, N.B. 2009. Insecticide potential of buprofezin and lambda-cyhalothrin on mealybugs, aphids and whitefly infesting ornamental-medicinal plants. Bull Ent. Soc. Egypt, Econ. Ser. 35(1): 189-201.

Wang, Z., Yan, H., Yang, Y. & Wu, Y. 2010. Biotype and insecticide resistance status of the whitefly Bemisia tabaci from China. Pest Manag. Sci. 66(12): 1360-1366.

Wheelock, C.E., Shan, G. & Ottea, J. 2005. Overview of carboxylesterases and their role in the metabolism of insecticides. J. Pestic. Sci. 30(2): 75-83.

Xie, W., Wang, S., Wu, Q., Feng, Y., Pan, H., Jiao, X., Zhou, L., Yang, X., Fu, W., Teng, H., Xu, B. & Zhang, Y. 2011. Induction effects of hosts plants on insecticide susceptibility and detoxification enzymes of Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Manag. Sci. 67(1): 87-93.

 

 

*Corresponding author; email: wintuma@live.com

 

 

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