Sains Malaysiana 43(3)(2014): 407–413

Development and Validation of Gas Chromatography-Mass Spectrometric Method for the Determination of Epoxidized Soybean Oil in Foods Stored in Glass Jars with Metal Lids

(Pembangunan dan Validasi Kaedah Kromatografi Gas-Spektrometri Jisim bagi Penentuan

Minyak Kacang Soya Terepoksi dalam Makanan yang Disimpan

di dalam Balang Kaca Berpenutup Logam)

HONG KEAT QUEK¹, MOHD MARSIN SANAGI^{1 2}*, WAN AINI WAN IBRAHIM¹,

SYAIRAH SALLEH¹& MAZIDATULAKMAM MISKAM^{1 2}

¹Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia

81310 UTM Johor Bahru, Johor, Malaysia

²Ibnu Sina Institute for Fundamental Science Studies, Universiti Teknologi Malaysia

81310 UTM Johor Bahru, Johor, Malaysia

Diserahkan: 28 Jun 2012/Diterima: 6 Julai 2013

ABSTRACT

A method has been developed for the determination of epoxidized soybean oil (ESBO) in oily foods stored in glass jars with metal lids using gas chromatography-mass spectrometry (GC-MS). ESBO and its internal standard (cis,cis-11,12;14,15-diepoxyeicosanoate) were isolated from the matrix by transesterification process. The developed method showed good linear dynamic range between 0.7-20 μg mL^-1 with coefficients of determination (R²) > 0.9968 and acceptable limit of detection and limit of quantitation of 7 and 23 mg kg^-1, respectively, based on linearity calculations. Analyte recoveries were 90.84±27.24% for low concentration, 78.05±11.59% for medium concentration and 99.23±10.20% for high concentration. This first fully validated GC-MS method was successfully applied for the determination of ESBO in foods stored in glass jar with metal lid. Among the 31 food samples studied, 6 samples were found to exceed the specific migration limit of 60 mg kg^-1 (based on EU Directive 2002/72/EC). The developed method is thus potentially useful for routine analysis for the determination of ESBO.

Keywords: Epoxidized soybean oil; foods stored in glass jars with metal lids; gas chromatography-mass spectrometry

ABSTRAK

Satu kaedah telah dibangunkan untuk menguji minyak kacang soya terepoksi (ESBO) dalam makanan berminyak di dalam balang kaca berpenutup logam menggunakan kromatografi gas-spektrometri jisim (GC-MS). ESBO dan piawai dalaman (cis,cis-11,12;14,15-diepoxyeicosanoate) telah dipisahkan daripada matrik menggunakan proses transesterifikasi. Kaedah yang dibangunkan ini menunjukkan dinamik linear yang baik antara 0.7-20 μg mL^-1 dengan pekali penentuan (R²) > 0.9968 dan had pengesanan serta had kuantifikasi yang boleh diterima iaitu 7 dan 23 mg kg^-1, masing-masing berdasarkan kepada pengiraan lineariti. Peratus pengembalian analit ialah 90.84±27.24%, bagi kepekatan rendah, 78.05±11.59% bagi kepekatan sederhana dan 99.23±10.20% bagi kepekatan tinggi. Kaedah GC-MS yang telah divalidasi sepenuhnya buat pertama kali telah berjaya diaplikasikan untuk penentuan ESBO dalam makanan yang disimpan di dalam balang kaca berpenutup logam. Daripada 31 sampel yang telah dikaji, 6 sampel didapati melebihi had 60 mg kg^-1 mengikut undang-undang Eropah (EU Directive 2002/72/EC). Oleh itu, kaedah ini berpotensi untuk digunakan sebagai analisis rutin untuk penentuan ESBO.

Kata kunci: Kromatografi gas-spektrometri jisim; makanan disimpan di dalam balang kaca berpenutup logam; minyak kacang soya epoksi

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*Pengarang untuk surat-menyurat; email: marsin@kimia.fs.utm.my