Sains Malaysiana 43(5)(2014):
715–722
In
vitro Regeneration and Acclimatization Protocols of Selected Ornamental
Plants
(Agapanthus praecox, Justicia betonica and Celosia
cristata)
(Protokol Regenerasi Secara In vitro dan Aklimatasi
Tumbuhan Hiasan Terpilih
(Agapanthus praecox, Justicia betonica dan Celosia
cristata))
JAMILAH SYAFAWATI YAACOB*1 2, AZANI
SALEH1 3, HASHIMAH
ELIAS1, SAKINAH
ABDULLAH1, NORAINI
MAHMAD1& NORMADIHA
MOHAMED1
1Institute
of Biological Sciences, Faculty of Science, University of Malaya
50603
Kuala Lumpur, Malaysia
2Centre for Research in Biotechnology for Agriculture (CEBAR), Institute
of Biological Sciences, Faculty of Science, University of Malaya,
50603 Kuala Lumpur, Malaysia
3School
of Applied Science, MARA University of Technology, 40450 Shah Alam,
Selangor, Malaysia
Diserahkan:
20 Mei 2013/Diterima: 4 September 2013
ABSTRACT
This paper discussed on the effectiveness of BAP and NAA growth hormones on
establishment of plant regeneration for selected ornamentals; Agapanthus praecox, Justicia betonica Linn.
and Celosia cristata. Various explants (leaf, stem, shoot tip and bulb)
derived from one-month-old aseptic seedlings of A. praecox and C.
cristata, as well as explants from intact plants of J. betonica were
utilized to achieve complete plant regeneration of these species. MS medium
supplemented with various hormones, with an emphasis on BAP and NAA were tested to obtain
direct and indirect regeneration. Both A. praecox (bulbs) and C.
cristata (shoots) formed complete plantlets on MS added with 0.5 –
2.0 mg/l BAP and NAA, while direct regeneration was achieved
for J. betonica on MS media containing BAP. Several methods were
attempted to acclimatize the regenerants, with A. praecox gave the
highest percentage of survival rates (96.67%), followed by J. betonica (80.00%)
and C. cristata (75.00%).
Keywords: Acclimatization; Agapanthus praecox; Celosia cristata; Justicia betonica; plant
growth regulators
ABSTRAK
Kertas ini menerangkan keberkesanan hormon pertumbuhan BAP dan NAA terhadap
regenerasi tumbuhan hiasan terpilih; Agapanthus praecox, Justicia betonica Linn. dan Celosia
cristata. Pelbagai eksplan (daun, batang, hujung pucuk dan ubi) yang berasal
daripada anak benih aseptik A. praecox dan C. cristata yang
berusia satu bulan, serta eksplan daripada tumbuhan utuh J. betonica telah
digunakan untuk menperoleh regenerasi lengkap spesies ini. Media MS yang
dibekalkan dengan pelbagai hormon, dengan penekanan kepada BAP dan NAA telah diuji untuk
mendapatkan regenerasi secara langsung atau tidak langsung. Kedua-dua A.
praecox (ubi) dan C. cristata (pucuk) membentuk anak pokok lengkap di
dalam media MS yang ditambah dengan 0.5 - 2.0 mg/L BAP dan NAA, manakala regenerasi
secara langsung telah dicapai oleh J. betonica di dalam media MS yang
mengandungi BAP.
Beberapa kaedah telah dicuba dalam aklimatasi anak pokok, dengan A. praecox memberikan peratusan kadar kelangsungan hidup tertinggi (96.67%), diikuti
oleh J. betonica (80.00%) dan C. cristata (75.00%).
Kata kunci: Agapanthus
praecox; Celosia cristata; hormon pertumbuhan; Justicia betonica;
pengikliman
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*Pengarang untuk surat-menyurat; email: jam_sya@yahoo.com
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