Sains Malaysiana 44(10)(2015): 1489–1500
Anti-malarial
and Anti-inflammatory Effects of Gynura procumbens are Mediated by Kaempferol via Inhibition of Glycogen Synthase
Kinase-3β (GSK3β)
(Kesan
Anti-malaria dan Anti-inflamasi Gynura procumbens Diperantara oleh
Kaempferol
melalui Perencatan
Glikogen Sintase Kinase-3β)
SOK
KUAN
WONG1,
MICHELLE
LEE
SUE
JANN1,
SUHAINI
SUDI1,
WAN
ROZIANOOR BT MOHD HASSAN1,
LEE PING
CHIN2,
NOOR EMBI1
& HASIDAH MOHD SIDEK1*
1School of Biosciences
and Biotechnology, Faculty of Science and Technology
Universiti Kebangsaan
Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia
2School of Science and
Technology, Universiti Malaysia Sabah, 88999 Kota Kinabalu, Sabah
Malaysia
Received: 27 November
2014/Accepted: 18 June 2015
ABSTRACT
Gynura procumbens is a medicinal
plant, traditionally used to treat inflammation and fever. A yeast-based
assay detected GSK3β-inhibitory activity in the aqueous extract of
G. procumbens. GSK3β is now known to have a central
role in the modulation of host inflammatory response during bacterial
infections. In this study, we investigated the involvement of GSK3β
in the anti-malarial and anti-inflammatory effects of an aqueous
extract of G. procumbens. Our results showed that G.
procumbens inhibited growth of P. falciparum 3D7. Consecutive
four-day administration of 250 mg/kg body weight (b.w.) G. procumbens
resulted in strong chemosuppression and improved survivability
in P. berghei-infected mice. B. pseudomallei-infected
mice treated with G. procumbens (50 mg/kg b.w.) showed increased
survivability. TNF-α and IFN-γ
levels in liver and serum of B.
pseudomallei-infected mice were lowered by G.
procumbens treatment. IL-10
level was higher in serum of G. procumbens-administered infected
mice. G. procumbens treatment of P. berghei- and B.
pseudomallei-infected animals each resulted in increased hepatic
GSK3β
(Ser9) phosphorylation. It is noteworthy that kaempferol (one of
the compounds in G. procumbens) also inhibited the growth of P.
falciparum 3D7; showed strong chemosuppression and improved survivability
in P. berghei-infected mice at 5 mg/kg b.w. B. pseudomallei-infected
mice treated with kaempferol (10 mg/kg b.w.) showed improved survivability.
Concomitantly, the described effects due to kaempferol also involved
enhanced GSK3β (Ser9) phosphorylation as
observed with G. procumbens. In summary, the observed anti-malarial
and anti-inflammatory effects of G. procumbens involved inhibition
of GSK3β
and kaempferol may in part be responsible for the pharmacological
effects.
Keywords: Anti-inflammation; anti-malaria; glycogen
synthase kinase-3β; Gynura procumbens; kaempferol
ABSTRAK
Gynura procumbens
merupakan tumbuhan ubatan yang digunakan secara tradisi untuk merawat
inflamasi dan demam. Pengasaian berasas-yis menunjukkan aktiviti
perencatan GSK3ß dalam ekstrak akueus G. procumbens.
GSK3ß kini diketahui berperanan dalam modulasi respons inflamasi
hos semasa infeksi bakteria. Kajian ini dijalankan untuk menentukan
penglibatan GSK3ß dalam kesan anti-malaria dan anti-inflamasi
ekstrak akueus G. procumbens. Hasil kajian menunjukkan G.
procumbens merencat pertumbuhan P. falciparum 3D7. Perlakuan
250 mg/kg berat tubuh (b.t.) G. procumbens selama empat hari
berturut-turut menunjukkan aktiviti kemo-penekanan yang kuat dan
peningkatan kemandirian dalam mencit terinfeksi-P. berghei.
Mencit terinfeksi-B. pseudomallei yang diberi perlakuan G. procumbens
(50 mg/kg b.t.) menunjukkan peningkatan kemandirian. Aras TNF-a
dan IFN-? dalam hepar dan serum mencit terinfeksi-B. pseudomallei
dicerap berkurangan dengan perlakuan G. procumbens. Aras
IL-10 didapati lebih tinggi dalam serum mencit terinfeksi yang diberi
perlakuan G. procumbens. Perlakuan G. procumbens terhadap
mencit terinfeksi-P. berghei dan B. pseudomallei masing-masing
menyebabkan peningkatan pemfosfatan GSK3ß (Ser9) hepar. Kaempferol
(salah satu sebatian/kompoun daripada G. procumbens) juga
mengakibatkan perencatan pertumbuhan P. falciparum 3D7; aktiviti
kemo-penekanan yang kuat dan peningkatan kemandirian mencit terinfeksi-P.
berghei pada dos 5 mg/kg b.t. Mencit terinfeksi-B. pseudomallei
yang diberi perlakuan kaempferol (10 mg/kg b.t.) menunjukkan peningkatan
kemandirian. Pada masa yang sama, kesemua kesan kaempferol tersebut
juga melibatkan peningkatan pemfosfatan GSK3ß (Ser9) seperti
yang dicerap dengan G. procumbens. Kesimpulannya, kesan anti-malaria
dan anti-inflamasi G. procumbens melibatkan perencatan GSK3ß;
dan kaempferol mungkin menyumbang kepada kesan farmakologi tersebut.
Kata
kunci: Anti-inflamasi;
anti-malaria; glikogen sintase kinase-3ß; Gynura
procumbens; kaempferol
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*Corresponding author; email: hasidah@ukm.edu.my
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