Sains Malaysiana 44(2)(2015): 275–280
Effects of Different Drying
Methods on the Antioxidant Activities of Leaves and Berries of Cayratia trifolia
(Kesan Kaedah Pengeringan yang Berbeza Terhadap Aktiviti Antioksida Daun dan Beri Cayratia trifolia)
M.S. RABETA*
& S.P. LIN
Food
Technology Division, School of Industrial Technology, Universiti Sains Malaysia
11800
Minden, Penang, Malaysia
Diserahkan: 8 Februari 2013/Diterima: 9 Julai 2014
ABSTRACT
This study aimed to
assess the effects of fresh, thermal drying method (vacuum oven
drying), and nonthermal drying method (freeze drying) on the antioxidant
activities of leaves and berries of Cayratia
trifolia using ferric reducing
antioxidant power (FRAP) and 1,1-diphenyl-1-picrylhydrazyl radical scavenging capacity
(DPPH) assays. The total phenolic
content (TPC)
and flavonoid content (TFC)
of the leaves and berries of C. trifolia were also measured.
Based on the results obtained, the TPC,
TFC, and antioxidant activities
of the leaves and berries were arranged in the following order:
freeze-dried sample with methanol extraction > vacuum-dried sample
with methanol extraction > freeze-dried sample with water extraction
> vacuum-dried sample with water extraction > fresh sample
with methanol extraction > fresh sample with water extraction.
The results showed a significant difference (p<0.05) between the fresh
and dried samples. In conclusion, freeze drying was found to be
a good method for maintaining TPC,
TFC, and antioxidant activities
by FRAP and DPPH methods in the leaves
and berries of C. trifolia.
Keywords: Cayratia trifolia; drying method; total antioxidant activity; total flavonoid; total phenolic
ABSTRAK
Kajian ini bertujuan
menilai kesan
kaedah pengeringan yang berbeza terhadap sampel segar, kaedah
pengeringan terma
(pengeringan ketuhar vakum) dan kaedah
pengeringan tak
terma (pengeringan beku) ke atas
aktiviti antioksida
daun dan beri
Cayratia trifolia yang diukur melalui asai kuasa antioksidan
penurunan ferik
(FRAP) dan
asai 1,1-difenil-1-pikrilhidrazil
(DPPH). Jumlah kandungan
fenolik (TPC)
dan flavonoid (TFC)
juga diukur.
Berdasarkan keputusan yang diperolehi, jumlah kandungan fenolik, flavonoid
dan jumlah aktiviti
antioksidan dalam
daun dan beri
boleh disusun
seperti berikut: Sampel yang dikering secara pembekuan dengan pengekstrakan metanol > sampel yang dikering secara oven vakum dengan pengekstrakan
metanol > sampel
yang dikering secara pembekuan dengan pengekstrakan air > sampel
yang dikering secara
oven vakum dengan pengekstrakan
air > sampel yang segar
dengan pengekstrakan metanol > sampel yang segar dengan pengekstrakan
air. Keputusan menunjukkan
perbezaan yang signifikan (p<0.05) antara sampel
segar dan
kering. Kesimpulannya,
pengeringan pembekuan dikenal pasti sebagai
kaedah yang baik
untuk mengekalkankan jumlah kandungan fenolik, flavonoid dan aktiviti antioksidan melalui kaedah FRAP
dan DPPH
dalam daun
dan beri C. trifolia.
Kata kunci: Cayratia trifolia; jumlah aktiviti antioksida; jumlah fenolik; jumlah flavonoid; kaedah pengeringan
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*Pengarang untuk surat-menyurat;
email: rabeta75@yahoo.com
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