Sains Malaysiana 44(3)(2015): 371–377
Occurrence
of Antibiotic Resistant Bacteria in Pond Water Associated with Integrated
Poultry-Fish Farming in Bangladesh
(Kejadian Bakteria Rintangan Antibiotik dalam Air Kolam Takungan
yang Berkaitan
dengan Penternakan Ayam-Ikan Bersepadu di Bangladesh)
FARZANA ASHRAFI NEELA1*, MST. NASRIN AKHTER BANU1, MD. ATIQUR RAHMAN1,
M. HABIBUR RAHMAN2 & M. FIROZ ALAM1
1Department
of Botany, Rajshahi University, Rajshahi 6205, Bangladesh
2Department
of Zoology, Rajshahi University, Rajshahi 6205, Bangladesh
Diserahkan:
14 Julai 2014/Diterima: 6 Oktober 2014
ABSTRACT
The use of antibiotics in poultry feed can cause antibiotic resistance
in integrated poultry-fish farming pond environment. Therefore,
the goal of this study was to monitor the prevalence of drug-resistant
bacteria in pond water associated with poultry-fish farming in Bangladesh.
The results showed that the prevalence rate of tetracycline (TC)
and ampicillin (AMP) resistant bacteria were 0.24 to
2.59% and 0.16 to 1.0%, respectively. The higher prevalence rate
of TC
and AMP-resistant
bacteria was found in site 3 followed by site 4 and 2 compared with
control site 1. Site 2, 3 and 4 were adjacent to the integrated
poultry farm. On the other hand, higher TC and AMP-resistant
bacteria were found in the rainy season than in the winter. The
minimum inhibitory concentration result showed quite high level
of TC and AMP-resistant
bacterial distribution in the pond water. Seven genera of resistant
bacteria were identified in pond water samples by 16S rRNA gene
analysis. Among them Acinetobacter sp. was predominant followed by Enterococcus sp., Pseudomonas sp., Bacillus,
Staphylococcus sp., Enterobacter sp. and Brevibacillus
sp.
Keywords: Antibiotics; integrated fish farm; poultry; resistant
bacteria
ABSTRAK
Penggunaan antibiotik dalam makanan ayam boleh menyebabkan penentangan
antibiotik dalam persekitaran kolam penternakan bersepadu ternakan
ikan. Oleh itu, matlamat kajian ini adalah untuk memantau kekerapan
bakteria rintangan drug dalam air kolam yang berkaitan dengan ternakan
ikan ternakan di Bangladesh. Keputusan menunjukkan bahawa kadar
prevalens tetrasiklin (TC)
dan bakteria rintangan-ampisilin (AMP) masing-masing adalah 0.24
kepada 2.59% dan 0.16 kepada 1.0%. Kadar kelaziman bakteria TC dan
rintangan-AMP yang tinggi didapati dalam tapak 3 yang diikuti oleh
tapak 4 dan 2 berbanding dengan kawalan tapak 1. Tapak 2, 3 dan
4 adalah bersebelahan dengan ladang ayam bersepadu. Selain itu,
bakteria TC dan
rintangan-AMP yang lebih tinggi ditemui dalam musim hujan daripada
pada musim sejuk. Hasil kepekatan rencatan minimum menunjukkan tahap
yang agak tinggi untuk pengagihan bakteria TC dan rintangan-AMP
di dalam air kolam. Tujuh genus bakteria rintangan
dikenal pasti dalam sampel air kolam oleh analisis gen 16 rRNA. Antaranya Acinetobacter sp. adalah pradominan diikuti
oleh bakteria Enterococcus sp., Pseudomonas sp., Bacillus,
Staphylococcus sp., Enterobacter sp. dan Brevibacillus
sp.
Kata kunci: Antibiotik; ayam; bakteria rintangan;
kolam ikan bersepadu
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*Pengarang
untuk surat-menyurat; email: nfarzanaashrafi@yahoo.com
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